Hpa002199

Manufactured by Merck Group

HPA002199 is a piece of laboratory equipment manufactured by Merck Group. It serves as a versatile tool for various scientific applications. The core function of this product is to facilitate precise measurement and analysis within a controlled laboratory environment. Detailed specifications and intended use are not provided in this factual description.

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Lab products found in correlation

Ab194356, by Abcam (1 mentions) Hpa001637, by Merck Group (1 mentions) Af806, by R&D Systems (1 mentions) Nbp1 44090, by Novus Biologicals (1 mentions) Ab24115, by Abcam (1 mentions) Hpa002380, by Merck Group (1 mentions)

2 protocols using hpa002199

Immunohistochemical Profiling of Vascular Markers

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IHC was performed on 6 μm sections of formalin-fixed, paraffin-embedded tissues. The sections were deparaffinized and dehydrated, and antigen retrieval followed. Then, the sections incubated with primary antibody toward CD31 (AF806, R&D Systems), CAVIN2 (NBP1-44090, Novus), HSPG2 (AF2364, R&D Systems), MYO1B (ab194356, Abcam), ABCG2 (ab24115, Abcam), SLC2A1 (HPA058494, Sigma-Aldrich), ABCB1 (HPA002199, Sigma-Aldrich), TJP1 (HPA001637, Sigma-Aldrich), CLDN5 (341600, Invitrogen), and PLVAP (NBP1-83911, Novus). All quantification was done by 2 individual scientists in a blinded fashion.

Xie Y., He L., Lugano R., Zhang Y., Cao H., He Q., Chao M., Liu B., Cao Q., Wang J., Jiao Y., Hu Y., Han L., Zhang Y., Huang H., Uhrbom L., Betsholtz C., Wang L., Dimberg A, & Zhang L. (2021). Key molecular alterations in endothelial cells in human glioblastoma uncovered through single-cell RNA sequencing. JCI Insight, 6(15), e150861.

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Immunolocalization of ABCB1 and ABCC1

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To immunolocalize ABCB1 and ABCC1, we used monospecific polyclonal antibodies generated in rabbits (ABCB1: Sigma HPA002199; ABCC1: Sigma HPA002380). Both antisera yielded very good and specific staining results and are known to specifically detect ABCB1 or ABCC1, resp. The specificity was recently also proven by us in Western blot analyses (Krohn et al., 2011 (link)).

Bernstein H.G., Hölzl G., Dobrowolny H., Hildebrandt J., Trübner K., Krohn M., Bogerts B, & Pahnke J. (2014). Vascular and extravascular distribution of the ATP-binding cassette transporters ABCB1 and ABCC1 in aged human brain and pituitary. Mechanisms of ageing and development, 0, 12-21.

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