Citrullus colocynthis (CC) fruits were collected from Wadi Hanifa in the Al-Diriyah region (Riyadh, Saudi Arabia) in February 2021. N-hexane (purity ≥ 99% (GC), Honeywell®, Seelze, Germany) was used as a solvent. Methanol (purity 99.8% (GC), Sigma Aldrich®, Steinheim, Germany), acetone (purity ≥ 99.5%, Sigma Aldrich®), aqueous hydrogen peroxide (30 wt.%, Sigma Aldrich®), glacial acetic acid (purity ≥ 99.8%, Fisher®Chemical, Loughborough, UK), sulphuric acid (95–98%, Panreac®, Barcelona, Spain), hydrochloric acid (37%, Panreac®), diethyl ether (purity ≥ 99.8%, Sigma Aldrich®), and anhydrous magnesium sulfate (purity ≥ 98%, Panreac®) were used as supplied. The reagents were required for the determination of hydroxyl and acid value such as potassium hydroxide (purity as KOH ≥ 85%, Loba Chemie®, Maharashtra, India), ethanol absolute (purity ≥ 99.97%, CHEM-LAB®, Zedelgem, Belgium), n-butanol (purity ≥ 99%, Loba Chemie®), pyridine (purity ≥ 99.5%, Loba Chemie®), acetic anhydride (purity ≥ 99.7%, VWR®, Fontenay-sous-Bois, France), and phenolphthalein indicator (purity ≥ 99%, Loba Chemie®). The materials required for film formation, such as BYK-333 as a surface tension reducer, were purchased from Al-Azzaz Chemicals company (Riyadh, Saudi Arabia) and 3HDI isocyanurate from Sigma Aldrich (Sigma Aldrich).
Anhydrous magnesium sulfate
Manufactured by ITW Reagents
Sourced in Spain
Anhydrous magnesium sulfate is a drying agent commonly used in chemical laboratories. It is a white crystalline solid that is highly effective at removing water from organic solvents and other liquids.
Automatically generated - may contain errors
Lab products found in correlation
Sulphuric acid, by ITW Reagents (1 mentions)
Aqueous hydrogen peroxide, by Merck Group (1 mentions)
Potassium hydroxide, by Loba Chemie (1 mentions)
Pyridine, by Loba Chemie (1 mentions)
Acetic anhydride, by Avantor (1 mentions)
Phenolphthalein indicator, by Loba Chemie (1 mentions)
Hydrochloric acid (hcl), by ITW Reagents (1 mentions)
Glacial acetic acid, by Thermo Fisher Scientific (1 mentions)
N butanol, by Loba Chemie (1 mentions)
Acetone, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Diethyl ether, by Merck Group (1 mentions)
N hexane, by Honeywell (1 mentions)
Anisodamine, by Merck Group (1 mentions)
Trisodium citrate dihydrate, by ITW Reagents (1 mentions)
Sodium citrate dibasic sesquihydrate, by Merck Group (1 mentions)
Magnesium sulfate, by ITW Reagents (1 mentions)
Milli q plus system, by Merck Group (1 mentions)
Atropine d5, by LGC (1 mentions)
Formic acid, by Merck Group (1 mentions)
3 protocols using anhydrous magnesium sulfate
1
Comprehensive Extraction and Analysis of Citrullus colocynthis
2
Quantification of Tropane Alkaloids
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Atropine ((±)-hyoscyamine), (±)-scopolamine hydrochloride, anisodamine, and homatropine hydrobromide were bought from Sigma-Aldrich as standards for tropane alkaloids (Madrid, Spain). For internal standards (IS), atropine-d5 and scopolamine-13C, d3 Hydrobromide were purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Before use, all standard solutions were maintained at room temperature for 30 min before being stored in amber vials in the dark at −20 °C.
Methanol (MeOH), acetonitrile (ACN), both HPLC-gradient grade, and formic acid were purchased from Merck (Darmstadt, Germany). Milli-Q plus system from Millipore (Molsheim, France) was used to purify the water, and it had a resistivity of 18.2 M cm.
For QuEChERS, magnesium sulfate and trisodium citrate dihydrate were purchased from PanReac (Barcelona, Spain), sodium chloride from Fluka (Seelze, Germany), and sodium citrate dibasic sesquihydrate from Sigma-Aldrich (Madrid, Spain). Agilent Technologies (Santa Clara, CA, USA) supplied the primary secondary amine-bonded silica (PSA) that was used for clean-up, in combination with anhydrous magnesium sulfate acquired from PanReac (Barcelona, Spain).
Methanol (MeOH), acetonitrile (ACN), both HPLC-gradient grade, and formic acid were purchased from Merck (Darmstadt, Germany). Milli-Q plus system from Millipore (Molsheim, France) was used to purify the water, and it had a resistivity of 18.2 M cm.
For QuEChERS, magnesium sulfate and trisodium citrate dihydrate were purchased from PanReac (Barcelona, Spain), sodium chloride from Fluka (Seelze, Germany), and sodium citrate dibasic sesquihydrate from Sigma-Aldrich (Madrid, Spain). Agilent Technologies (Santa Clara, CA, USA) supplied the primary secondary amine-bonded silica (PSA) that was used for clean-up, in combination with anhydrous magnesium sulfate acquired from PanReac (Barcelona, Spain).
3
Quantitative Analysis of Atropine and Hyoscyamine
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Atropine reference standard was purchased from Sigma-Aldrich (St. Louis, MO, USA) and (-)hyoscyamine reference standard was obtained from USP (Teddington, Middlesex, United Kingdom). Stock standard solutions of atropine and (-)-hyoscyamine (200 mg/L) were individually prepared by exact weighing of the solid substances and dissolved in 50 mL of ethanol (HPLC grade, J.T. Baker, Deventer, Holland), and they were stored at -20 °C in the dark. A mixture working standard solution was prepared at 5 mg/L with ethanol and stored in screw-capped glass tubes at -20 °C in the dark.
HPLC grade acetonitrile, isopropanol, acetic acid and acetone were obtained from J.T. Baker.
Formic acid (Optima LC-MS), ammonium formate and diethanolamine (DEA) were obtained from Fisher Scientific (Geel, Belgium). Methanol was obtained from Fluka (Steinheim, Suiza), while nhexane was obtained from Scharlab (Barcelona, Spain). Triethylamine was purchased from Riedelde Häen (Hannover, Germany). Graphitized black carbon (GBC) and primary secondary amine (PSA) were purchased from Scharlab (Barcelona, Spain). Anhydrous magnesium sulfate and ammonium acetate were purchased from Panreac (Barcelona, Spain). Ultrapure water was obtained by a gradient system Milli-Q water (Millipore, Bedford, MA, USA).
HPLC grade acetonitrile, isopropanol, acetic acid and acetone were obtained from J.T. Baker.
Formic acid (Optima LC-MS), ammonium formate and diethanolamine (DEA) were obtained from Fisher Scientific (Geel, Belgium). Methanol was obtained from Fluka (Steinheim, Suiza), while nhexane was obtained from Scharlab (Barcelona, Spain). Triethylamine was purchased from Riedelde Häen (Hannover, Germany). Graphitized black carbon (GBC) and primary secondary amine (PSA) were purchased from Scharlab (Barcelona, Spain). Anhydrous magnesium sulfate and ammonium acetate were purchased from Panreac (Barcelona, Spain). Ultrapure water was obtained by a gradient system Milli-Q water (Millipore, Bedford, MA, USA).
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