Assaymax rat adiponectin elisa kit

Standard rat chow (Gold Coin, Selangor, Malaysia), high fat diet (MP Diets, USA), tween 20, ethanol, biochemical kits for total cholesterol, triglyceride, LDL-c, and HDL-c were procured from Randox (Roche Diagnostics GmbH, Sandhofer Strasse, Mannheim), insulin, leptin, ghrelin and adiponectin levels were obtained from Mercodia Rat Insulin ELISA Kit, Uppsala, Sweden, RayBio Rat Leptin ELISA kit, Norcross, GA, USA, AssayMax Rat Adiponectin ELISA Kit, and RayBio Rat Ghrelin Enzyme Immunoassay Kit, Norcross, GA, USA. All the solvents, reagents and chemical used in the present study were of analytical grade.

Rats were fasted for 12 h before blood glucose measurement. Food was removed from the food dispenser and bedding of the cages was changed to avoid coprophagy. Blood from the tail vein was measured for glucose using the OneTouch Basic glucose monitor (Lifescan) and reported as mmol/L. Blood glucose level was measured during the study and prior to sacrifice. TC, LDL, HDL, TG, urea nitrogen, creatinine, GGT, ALT, AST, and ALP activity were measured on a Roche/Hitachi cobac C system and results were expressed in U/L (Roche Diagnostics GmbH, Sandhofer, Strasse, Mannheim), following manufacturers’ protocols. Plasma leptin, insulin, adiponectin, and ghrelin were determined using enzyme-linked immunoabsorbent assay (ELISA) kits following manufacturer’s instruction (leptin: RayBio® Rat Leptin ELISA Kit, Cat # ELR-Leptin-001, Norcross, GA, USA; insulin: Mercodia Rat Insulin ELISA, Uppsala, Sweden; adiponectin: Assay-Max Rat Adiponectin ELISA kit, Cat# ERA2500-1; ghrelin: RayBio® Rat Ghrelin ELISA kit, Cat# EIA-GHR-1, Norcross, GA, USA).