Graphitized carbon cartridges

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

Graphitized carbon cartridges are a type of solid-phase extraction (SPE) media used for the purification and concentration of analytes from complex samples. They are made of porous graphitized carbon, which provides a large surface area for the adsorption of organic compounds. These cartridges can be used to extract a wide range of organic compounds, including polar and non-polar species, from various sample matrices such as water, biological fluids, and environmental samples.

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Lab products found in correlation

Maxis 4 g q tof ms, by Bruker (1 mentions) Goat anti rabbit igg conjugated with horseradish peroxidase hrp, by Santa Cruz Biotechnology (1 mentions) Extravidin peroxidase, by Merck Group (1 mentions) Cnbr activated sepharose 4b, by Cytiva (1 mentions) Peptide n glycosidase f, by New England Biolabs (1 mentions)

2 protocols using graphitized carbon cartridges

N-Glycan Fractionation and Analysis

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Reducing N‐glycans were fractionated on a TSK‐amide 80 column (Tosoh Bioscence GmbH, www.tosohbioscience.com) as described (Grass et al., 2011), except that underivatized glycans were used. Therefore, fractions were analyzed for glycans by MALDI‐TOF MS.
For analytical purposes, the glycans were reduced with NaBH₄, desalted by passage over graphitized carbon cartridges (ThermoFisher Scientific, www.thermofisher.com) and subjected to LC‐ESI‐MS with a PGC column (0.32 μm × 150 mm) operated by an ultimate rapid separation LC (RSLC) system (ThermoFisher Scientific) connected to a Maxis 4 G Q‐TOF MS (Bruker) or an amaZone ion trap (Bruker), operating in data‐dependent acquisition mode (Pabst et al., 2012). The PGC column was eluted with 65 mm ammonium formate at pH 3.0 and a gradient from 8 to 60% acetonitrile in 50 min at a flow rate of 6 µl min⁻¹ at ambient temperature.

Mócsai R., Figl R., Sützl L., Fluch S, & Altmann F. (2020). A first view on the unsuspected intragenus diversity of N‐glycans in Chlorella microalgae. The Plant Journal, 103(1), 184-196.

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Lectin-based Glycoprotein Isolation and Analysis

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Four biotinylated lectins were purchased from Vector Laboratories (Burlingame, CA, USA). Commercial human serum and ExtrAvidin Peroxidase were purchased from Sigma (St. Louis, MO, USA). The Rabbit anti-human Hp antibody (Ab) was obtained from Dako (Carpinteria, CA, USA). Goat anti-rabbit IgG conjugated with horseradish peroxidase (HRP) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). CNBr-activated Sepharose 4B was acquired from Amersham Bioscience (Minneapolis, MN, USA). Peptide N-glycosidase F was purchased from New England Biolabs (Ipswich, Suffolk, UK). Graphitized carbon cartridges were purchased from Thermo Fisher Scientific (Runcorn, Cheshire, UK). MS calibrant solution (ESI-TOF Calibrant Mix G1969-85000) was procured from Agilent Technologies (Santa Clara, CA, USA). All other reagents were of analytical grade or higher.

Lee S.H., Jeong S., Lee J., Yeo I.S., Oh M.J., Kim U., Kim S., Kim S.H., Park S.Y., Kim J.H., Park S.H., Kim J.H, & An H.J. (2016). Glycomic profiling of targeted serum haptoglobin for gastric cancer using nano LC/MS and LC/MS/MS. Molecular bioSystems, 12(12).

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