Galactosylceramide

AG-205 (cis-2-[[1-(4-Chlorophenyl)-1H-tetrazol-5-yl]thio]-1-(1,2,3,4,4a,9b-hexahydro-2,8-dimethyl-5H-pyrido[4,3-b]indol-5-yl)-ethanone) (cat# 6242) was obtained from Bio-Techne (Minneapolis, MN, USA), dissolved in DMSO (cat# 1.02931, Merck, Darmstadt, Germany) at a concentration of 10 mM, and stored in aliquots at −20 °C. Brefeldin A (BFA) (cat# B6542) was purchased from Sigma-Aldrich (St. Louis, MO, USA), dissolved in ethanol at a final concentration of 5 mg/mL, and stored at −20 °C. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) (cat# M5655) was purchased from Sigma-Aldrich, dissolved in water (10 mg/mL), and stored at −20 °C. Chloroform (cat# 7331) and methanol (cat# AE71) were obtained from Carl Roth (Karlsruhe, Germany). The following lipid standards were purchased from Sigma-Aldrich (St. Louis, MO, USA): cholesterol (cat# C8667), galactosylceramide (cat# C4905), phosphatidylcholine (cat# P3556), sphingomyelin (cat# S7004), and sulfatides (cat# S1006). C16 lactosylceramide (d18:1/16:0) (cat# 860576) and 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (cat# 850702) were from Avanti Polar Lipids (Alabaster, AL, USA). All chromatographies were performed using silica gel 60 high-performance thin-layer chromatography (HPTLC) plates (cat# 1.05641, Merck, Darmstadt, Germany).