The largest database of trusted experimental protocols

34 protocols using amylose amylopectin assay kit

1

Potato Starch Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Potato starch powder (S4251, CAS: 9005-25-8, Sigma Aldrich, Steinheim, Germany) was utilized in this experimental work. Before its use, it was characterized by chemical and physical measurements. Potato starch composition was 13.5% moisture content (determined according to the method 925.10, AOAC, 2005), 0.8% fat (determined according to the method 922.06, AOAC, 2005), 0.3% ash (determined according to the method 923.03, AOAC, 2005) and 25% amylose (determined by Megazyme amylose/amylopectin assay kit, Megazyme International Ireland Ltd., Wicklow, Ireland).
Potato starch particle size distribution was evaluated by dynamic light scattering (DLS), using a Malvern Mastersizer 2000 instrument (Malvern Instruments Ltd., Worcestershire, UK). A monomodal distribution was determined with a D [4.3] of 48.75 µm, and a volumetric distributions of 3% of d(0.1) = 19.53 µm, 10.5% of d(0.5) = 45.52 µm and 5.66% of d(0.9) = 89.81 µm.
+ Open protocol
+ Expand
2

Determination of Amylose and Starch Content

Check if the same lab product or an alternative is used in the 5 most similar protocols
The amylose and total starch content of isolated anchote starches, as well as potato and cassava starches, were determined using the Megazyme amylose/amylopectin assay kit (Megazyme, Bray Business Park, Bray, Ireland), according to the manufacturer’s instructions.
+ Open protocol
+ Expand
3

Wheat Flour Composition Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ground meal samples obtained from control wheat and FB- (flour/starch/protein concentrate/protein isolate) supplemented crackers were used to determine the total starch concentration [40 ], protein [41 ], crude fat concentration [42 ] and total dietary fiber (TDF) [43 ] as previously described. [15 (link)]. Amylose concentration in these samples was determined by a concanavalin A method using a commercial kit (Megazyme Amylose/Amylopectin Assay Kit, Megazyme, Wicklow, Ireland) following the manufacturer’s instructions.
Total raffinose family oligosaccharides (RFO) concentration (mmol/100 g on fresh weight basis) in all samples was determined by stepwise enzymatic hydrolysis using a commercial assay kit (K-RAFGL 04/18, Megazyme International Ireland Ltd., Wicklow, Ireland) [44 (link)]. For quantitative RFO determination from, an alcohol-based extraction followed by purification, with C18 column was used. A CarboPac PA100 column attached to HPAEC-PAD (High Performance Anion Exchange Chromatography with Pulsed Amperometric Detector; Dionex Canada Ltd., Oakville, ON, Canada) was used to separate the soluble sugars and determine the respective RFO concentration as described [45 (link)].
+ Open protocol
+ Expand
4

Amylose Content Determination in RS

Check if the same lab product or an alternative is used in the 5 most similar protocols
The amylose content in the RS samples was determined using a Megazyme Amylose/Amylopectin assay kit (Megazyme Ltd., Winklow, Ireland) according to the manufacturer instructions.
+ Open protocol
+ Expand
5

Amylose Content Phenotyping in Interspecific Progenies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phenotyping for AC was carried out on a total of 100 interspecific progenies together with their parental lines. About 60 seeds were first dehusked and then milled to fine flour using the (QIAGEN tissuelyser, Hilden, Germany). AC was measured using the Megazyme amylose/amylopectin assay kit (Megazyme International Ltd., Bray, Ireland) according to manufacturer's procedure.
+ Open protocol
+ Expand
6

Analytical Methods for Biofuel Precursors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hydrogen and organic acids were determined by gas chromatography (GC-2014C, Shimadzu, Kyoto, Japan), equipped with a thermal conductivity detector, as detailed in a previous study67 (link). The ratio of α-1,4/1,6 oligosaccharides was determined with the Amylose/Amylopectin Assay Kit (Megazyme, Wicklow, Ireland). Organic acids and glucose were analyzed by high-performance liquid chromatography with refractive index and ultraviolet detectors and a Bio-Rad Aminex HPX-87H column. The analysis was performed using 5 mM sulfuric acid as the mobile phase at 55 °C with a flow rate of 0.5 mL min−1. The total starch concentration was determined by the DNS method.
+ Open protocol
+ Expand
7

Comprehensive Rice Grain Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The RS content was determined using the AOAC official method 2002.02, which has been described by Ren et al. [5] (link). The contents of total starch and amylose/amylopectin were measured with total starch assay kit and amylose/amylopectin assay kit (Megazyme International, Ireland, Ltd), respectively. Other basic components were carried out by following the AOAC protocol (AOAC, 2000).
+ Open protocol
+ Expand
8

Starch and Amylose Analysis in Mutant Rice

Check if the same lab product or an alternative is used in the 5 most similar protocols
The seeds of 7, 14, 21, 28 and 35 DAF of mutant line and wild-type Gao 8901 were used to carry out starch content, amylose content and GBSS I activity analysis. Total starch and amylose contents were measured using the Total Starch Assay Kit and Amylose/Amylopectin Assay Kit (www.megazyme.com) according to manual instructions. GBSS I activity was measured using the Micro Bound Station amylosynthease Assay Kit (http://www.shkxbio.com). All tests were performed on three replicates. Student’s t-test assessed statistical differences between wild type and mutant line.
+ Open protocol
+ Expand
9

Comprehensive Chemical Characterization of Pasta

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemical composition was assessed both on raw materials and dry pasta. Moisture was measured by a thermobalance (Sartorius MA 40, Goettingen, Germany) at 120 °C and all analytical data were expressed as dry weight (dw).
Total starch (TS) content was determined according to the Official Method 996.11 [28 ], by Total Starch Assay Kit (Megazyme, Bray, Ireland). Amylose content was determined using the Megazyme Amylose/Amylopectin assay kit. Resistant starch (RS) content was determined according to the Official Method 2002.02 [29 ], using Resistant Starch Assay Kit (Megazyme). Total dietary fiber (TDF) content was measured using the enzymatic kit Bioquant (Merck, Darmstadt, Germany) according to the Official Method 991.42 [30 ]. Ash content was determined according to the Official Method 08-01.01 [31 ]. Protein content was determined by micro-Kjeldhal nitrogen analysis, according to the ICC 105-2 method [32 ], using as conversion factor N × 6.25. Total antioxidant capacity (TAC) was determined according to Martini et al. [33 (link)].
+ Open protocol
+ Expand
10

Starch Isolation and Characterization of Rice Cultivars

Check if the same lab product or an alternative is used in the 5 most similar protocols
Starch isolation. The starch was isolated from the flour of 4 representative rice cultivars via alkaline extraction as previously described [2 (link)]. The isolated starch samples were thoroughly ground, sifted through a 35 mesh (i.e., 0.50 mm) sieve (Brass Frame Stainless Mesh, Humboldt Mfg., Co., Raleigh, NC, USA), and kept at 4 °C until further analyses [2 (link)]. Nitrogen (N) and protein content. The N content of purified starch was estimated by using the Kjeldahl method according to the AOAC method 920.87 [28 ]. N values were then converted to protein content by N × 5.95. Moisture content. The moisture content was measured according to AACC Air Oven Method 44-19 [29 ] using a hot air oven (Binder Inc., Tuttlingen, Germany) at 135 °C for 2 h. Amylose content. Approximately 20 mg of the isolated starch was used to determine amylose content by using Amylose/Amylopectin Assay Kit (Megazyme International Ireland Ltd., Wicklow, Ireland) following the manufacturer’s recommendations.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!