Potato starch particle size distribution was evaluated by dynamic light scattering (DLS), using a Malvern Mastersizer 2000 instrument (Malvern Instruments Ltd., Worcestershire, UK). A monomodal distribution was determined with a D [4.3] of 48.75 µm, and a volumetric distributions of 3% of d(0.1) = 19.53 µm, 10.5% of d(0.5) = 45.52 µm and 5.66% of d(0.9) = 89.81 µm.
Amylose amylopectin assay kit
The Amylose/Amylopectin Assay Kit is a laboratory tool designed to quantify the levels of amylose and amylopectin in starch samples. It provides a method to determine the ratio of these two polysaccharides, which are the main components of starch.
Lab products found in correlation
34 protocols using amylose amylopectin assay kit
Potato Starch Characterization Protocol
Potato starch particle size distribution was evaluated by dynamic light scattering (DLS), using a Malvern Mastersizer 2000 instrument (Malvern Instruments Ltd., Worcestershire, UK). A monomodal distribution was determined with a D [4.3] of 48.75 µm, and a volumetric distributions of 3% of d(0.1) = 19.53 µm, 10.5% of d(0.5) = 45.52 µm and 5.66% of d(0.9) = 89.81 µm.
Determination of Amylose and Starch Content
Wheat Flour Composition Analysis
Total raffinose family oligosaccharides (RFO) concentration (mmol/100 g on fresh weight basis) in all samples was determined by stepwise enzymatic hydrolysis using a commercial assay kit (K-RAFGL 04/18, Megazyme International Ireland Ltd., Wicklow, Ireland) [44 (link)]. For quantitative RFO determination from, an alcohol-based extraction followed by purification, with C18 column was used. A CarboPac PA100 column attached to HPAEC-PAD (High Performance Anion Exchange Chromatography with Pulsed Amperometric Detector; Dionex Canada Ltd., Oakville, ON, Canada) was used to separate the soluble sugars and determine the respective RFO concentration as described [45 (link)].
Amylose Content Determination in RS
Amylose Content Phenotyping in Interspecific Progenies
Analytical Methods for Biofuel Precursors
Comprehensive Rice Grain Analysis
Starch and Amylose Analysis in Mutant Rice
Comprehensive Chemical Characterization of Pasta
Total starch (TS) content was determined according to the Official Method 996.11 [28 ], by Total Starch Assay Kit (Megazyme, Bray, Ireland). Amylose content was determined using the Megazyme Amylose/Amylopectin assay kit. Resistant starch (RS) content was determined according to the Official Method 2002.02 [29 ], using Resistant Starch Assay Kit (Megazyme). Total dietary fiber (TDF) content was measured using the enzymatic kit Bioquant (Merck, Darmstadt, Germany) according to the Official Method 991.42 [30 ]. Ash content was determined according to the Official Method 08-01.01 [31 ]. Protein content was determined by micro-Kjeldhal nitrogen analysis, according to the ICC 105-2 method [32 ], using as conversion factor N × 6.25. Total antioxidant capacity (TAC) was determined according to Martini et al. [33 (link)].
Starch Isolation and Characterization of Rice Cultivars
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