H7509

Manufactured by Merck Group

Sourced in Indonesia

The H7509 is a laboratory equipment product from Merck Group. It is designed for general laboratory use. The core function of this product is to provide a standardized and reliable platform for various laboratory applications. Further details on the specific intended use of this product are not available.

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Lab products found in correlation

P4780, by Merck Group (1 mentions) Cd 1 nude female mice, by Charles River Laboratories (1 mentions) Selumetinib, by Selleck Chemicals (1 mentions) Selumetinib, by Merck Group (1 mentions) Trametinib, by Merck Group (1 mentions) P1754, by Merck Group (1 mentions) Ftp 20 38 50, by Instech (1 mentions) Cyt387, by Chemietek (1 mentions) Hydroxypropyl methylcellulose, by Merck Group (1 mentions) Cld 8901, by Encapsula Nanosciences (1 mentions)

6 protocols using h7509

Nanohydroxyapatite-Gelatin Scaffold for Tissue Engineering

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Nanohydroxyapatite used in this study was obtained from Badan Tenaga Nuklir Nasional (BATAN) Jakarta, Indonesia, originated from the fish scales. The gelatin was derived from cow skin purchased from 150 bloom Rousselot (Guangdong, China). The streptomycin sulphate (powder for injection) was obtained from PT. Meiji Indonesia. The hydroxypropyl methylcellulose (HPMC) was obtained from Sigma Aldrich H7509.
The materials used for characterization were hydroxyapatite scaffold from Tissue Bank General Hospital Dr. Soetomo (Surabaya, Indonesia) for setting time test, NaCl, NaHCO₃, KCl, K₂HPO₄.3H₂O, MgCl₂.6H₂O, HCl, CaCl₂.2H₂O, Na₂SO₄ dan (HOCH₂)₃CNH₂ for SBF solution and Staphylococcus aureus for antibacterial test.
The tools used were freezer and lyophilizer, 10 cc syringe, viscotester VT-04F RION, pH meter Benchtop OAKTON, Scanning Electron Microscope (SEM) FEI Inspect S50 Japan, and UV-Vis Spectrometer.

Hikmawati D., Maulida H.N., Putra A.P., Budiatin A.S, & Syahrom A. (2019). Synthesis and Characterization of Nanohydroxyapatite-Gelatin Composite with Streptomycin as Antituberculosis Injectable Bone Substitute. International Journal of Biomaterials, 2019, 7179243.

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Systemic DBZ Administration in Mice

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A stock solution of 300 mM DBZ (Tocris 4489) in DMSO was prepared and stored at 4°C with light protection. For injection, the DBZ stock solution (or DMSO alone for vehicle control) was diluted to 3 mM DBZ with Tween 80 (Sigma P4780, final concentration 0.1% v/v) and (hydroxypropyl)methyl cellulose (Sigma H7509, final concentration 0.5% w/v), then administered i.p. to adult mice in daily DBZ doses of 30 μmol per kg body weight (10 μl per g body weight) for six consecutive days before harvesting on day seven for analysis.

Ouadah Y., Rojas E.R., Riordan D.P., Capostagno S, & Krasnow M.A. (2019). A subpopulation of pulmonary neuroendocrine cells are reserve stem cells regulated by the tumor suppressors Rb, p53, and Notch. Cell, 179(2), 403-416.e23.

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Colorectal Tumor Xenograft Mouse Model

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CD-1 female nude mice (obtained from Charles River, 7–9 weeks old) received unilateral subcutaneous injections of 100 µl of HCT116 cells (2 × 10⁶ cells) or 100 µl of DLD-1 cells (4 × 10⁶ cells) suspended in PBS. Mice were placed on experimental diets (control or -SG) 10 days (for HCT116 xenograft experiment) or 2 days (for DLD-1 xenograft experiment) after tumour injections. In all, 4 days (for HCT116 xenograft experiment) or 2 days (for DLD-1 xenograft experiment) after the diet change, mice were treated either with vehicle (0.5% methylcellulose (Sigma, H7509), 0.5% Tween-80 (Sigma, P8192)) or PH755 (obtained from Raze Therapeutics) prepared in vehicle once daily by oral gavage. The starting dosage of PH755 was 100 mg/kg and was subsequently lowered to 75 mg/kg or 50 mg/kg as indicated in the figure legends. Subcutaneous growth was measured two to three times a week by caliper and the following formula: length × width²/2 was used to calculate tumour volume.

Tajan M., Hennequart M., Cheung E.C., Zani F., Hock A.K., Legrave N., Maddocks O.D., Ridgway R.A., Athineos D., Suárez-Bonnet A., Ludwig R.L., Novellasdemunt L., Angelis N., Li V.S., Vlachogiannis G., Valeri N., Mainolfi N., Suri V., Friedman A., Manfredi M., Blyth K., Sansom O.J, & Vousden K.H. (2021). Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy. Nature Communications, 12, 366.

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Sigma-1 Receptor Ligands in Neuropathic Pain

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The following selective Sig-1R ligands were used: PRE-084 at a dose of 0.25 mg/kg (0589,TOCRIS; Bristol, UK), EST79232 and EST79376 at doses of 0.5 and 5 mg/kg (synthesized and supplied by ESTEVE Pharmaceuticals). The compounds were dissolved in 0.5% hydroxypropyl-methylcellulose (HPMC; H7509, Sigma-Aldrich) in distilled water and were administered by intraperitoneal (i.p.) route twice daily (bid) in a volume of 10 mL/kg. Administrations were given from 30 min after rhizotomy surgery until 42 days post-injury (dpi), the end of the study; in the SOD1^G93A study, treatments were given from 8 to 16 weeks of age (see Figure 6).
For the spinal nerve injury (rhizotomy, rhizo), female WT mice were distributed in the following experimental groups: uninjured control (CTL) (n = 16), rhizo + vehicle 0.5% HPMC (n = 14), rhizo + PRE-084 0.25 mg/kg (n = 5), rhizo + EST79232 0.5 mg/kg (n = 5), rhizo + EST79232 5 mg/kg (n = 6), rhizo + EST79376 0.5 mg/kg (n = 5), rhizo + EST79376 5 mg/kg (n = 5). For the SOD1^G93A studies, male transgenic mice were divided into 4 groups: SOD1+ vehicle 0.5% HPMC (n = 13), SOD1 + PRE-084 0.25 mg/kg (n = 5), SOD1 + EST79232 5 mg/kg (n = 7), SOD1+ EST79376 5 mg/kg (n = 6), with B6SJL male WT age-matched mice used as negative control of the disease (n = 13).

Gaja-Capdevila N., Hernández N., Yeste S., Reinoso R.F., Burgueño J., Montero A., Merlos M., Vela J.M., Herrando-Grabulosa M, & Navarro X. (2022). EST79232 and EST79376, Two Novel Sigma-1 Receptor Ligands, Exert Neuroprotection on Models of Motoneuron Degeneration. International Journal of Molecular Sciences, 23(12), 6737.

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Selumetinib and Trametinib Oral Gavage

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Selumetinib (AZD6244, S1008) was purchased from Selleckchem and dissolved in DMSO at stock dilutions of 30 mg/mL under sterile conditions.
Working dilutions of Trametinib and Selumetinib were dissolved at a dose volume of 0.2 mL/20 g body weight in 0.5% hydroxypropylmethylcellulose (Sigma-Aldrich H7509), 0.2% Tween-80 (Sigma-Aldrich P1754) in distilled water (pH 8.0). Adult mice were treated at specific concentrations of Trametinib or Selumetinib by oral gavage (plastic feeding tubes, 20ga x 38 mm, Instech, FTP-20-38-50), every day for 14 days, and then sacrificed at 4 h post dose. Control mice were treated with vehicle only (DMSO).

Angelini G., Capra E., Rossi F., Mura G., Saclier M., Taglietti V., Rovetta G., Epis R., Careccia G., Bonfanti C, & Messina G. (2023). MEK-inhibitors decrease Nfix in muscular dystrophy but induce unexpected calcifications, partially rescued with Cyanidin diet. iScience, 27(1), 108696.

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Tumor Formation and Modulation Studies

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For tumor formation studies, 1 × 10⁶ cells in PBS were injected into the left and right flank of each mouse. Thus, each mouse contributed two tumors for each condition [shControl (shCT) and shDAB2IP] and (CNT, DAB2IP, R289L, S604A, IκBαSR) such that there were four mice and eight tumors per condition. Tumor size was measured every 2 to 3 days by calipers. Tumor volume was calculated using the standard formula L × W² × 0.52.
For the cytokine inhibitor study, 1 × 10⁶ shDAB2IP cells in PBS were injected into the left and right flank of each mouse (n = 16), for a total of 32 injections. One day prior to cell implantation, mice were administered 50 mg/kg of CYT-387 (ChemieTek, catalog no. CT-CYT387) or vehicle [0.5% (w/v) hydroxypropyl methylcellulose (Sigma Aldrich, catalog no. H7509), 0.1% Tween 80, pH 8.0] by oral gavage. Treatment was administered daily. Tumor volume was calculated and graphed.
For the macrophage depletion study, 1 × 10⁶ shDAB2IP cells in PBS were injected into the left and right flank of each mouse (n = 37), for a total of 74 injections. 3 days prior to cell implantation, mice were administered 1 mg of Chlodrosome or the control Encapsome (Encapsula NanoSciences, catalog no. CLD-8901) through intravenous tail vein injection. Treatment was administered every 3 days.

Miller A.L., Perurena N., Gardner A., Hinoue T., Loi P., Laird P.W, & Cichowski K. (2023). DAB2IP Is a Bifunctional Tumor Suppressor That Regulates Wild-Type RAS and Inflammatory Cascades in KRAS Mutant Colon Cancer. Cancer Research, 83(11), 1800-1814.

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