Acetonitrile (HPLC super gradient grade) and methanol (HPLC super gradient grade) were purchased from Lab-Scan (Dublin, Ireland). Hydrochloric acid p.a. (36.5%) was a product of Ultrapure water produced by a Milli-Q Plus system (Millipore Corporation, Burlington, MA, USA). The AccQ•TagReagent Kit was purchased from Waters (Milford, MA, USA). The reagent kit consists of Waters AccQ•Fluor Borate Buffer, Waters AccQ•Fluor Reagent Powder (6-aminoquinolyl-N-hydroxy-succinimidyl carbamate—AQC), Waters AccQ•Fluor Reagent Diluent, Waters AccQ•Tag Amino Acid Analyzing Column (Nova-Pak C18, 4 µL, 150 × 3.9 mm), and Waters Amino Acid Hydrolysate Standard (each ampoule contains a 2.5 mM mixture of the 17 hydrolysate amino acids except for cystine—1.25 mM), i.e., aspartic acid (Asp), serine (Ser), glutamic acid (Glu), glycine (Gly), histidine (His), arginine (Arg), threonine (Thr), alanine (Ala), proline (Pro), cysteine (Cys), tyrosine (Tyr), valine (Val), methionine (Met), lysine (Lys), isoleucine (Ile), leucine (Leu), and phenylalanine (Phe)).
Waters accq fluor reagent diluent
Manufactured by Waters Corporation
The Waters AccQ•Fluor Reagent Diluent is a laboratory reagent used to dilute and reconstitute the AccQ•Fluor reagent. It provides a consistent and reliable environment for the derivatization process.
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2 protocols using waters accq fluor reagent diluent
1
Amino Acid Analysis Using AccQ•Tag Reagent Kit
2
HPLC-based Amino Acid Quantification
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Acetonitrile of HPLC grade and 18 amino acid standards, including glycine (Gly), alanine (Ala), serine (Ser), cysteine (Cys), threonine (Thr), valine (Val), leucine (Leu), isoleucine (Iso), methionine (Met), phenylananine (Phe), tryptophan (Try), tyrosine (Tyr), aspartic acid (Asp), glutamic acid (Glu), glutamine (Gln), lysine (Lys), arginine (Arg), and histidine (His), were purchased from Sigma-Aldrich (St. Louis, MO, USA). The derivatizing reagent (kit Waters AccQ·Fluor™) and eluent A were supplied by Waters (Massachusetts, USA). Waters AccQ·Fluor™ kit consists of Waters AccQ·Fluor borate buffer, Waters AccQ·Fluor reagent powder, and Waters AccQ·Fluor reagent diluent. HPLC grade water was obtained from a Milli-Q system (Millipore, Bedford, MA, USA). Cellulose acetate filters (0.45 µm) were supplied by Anpel (Shanghai, China).
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