1,1′-Carbonyldiimidazole (CDI), dextranase (Penicillium sp.), and 3,5-dinitrosalicylic acid (DNS) were purchased from Sigma Chemical Co. (St Louis, MO, USA). Succinic anhydride and 5-benzyl N-(Boc)-glutamate were purchased from Tokyo Chemical Industry (Tokyo, Japan). Dextran (molecular weight: 15–20 kDa) was purchased from Fluka (Sigma Chemical Co.). Celecoxib was ether-extracted from Celebrex capsules (Pfizer, Inc., New York, NY, USA). All other chemicals were reagent grade, commercially available products. Buffer solutions (pH 1.2 and 6.8) were prepared as described in USP XXIII. Thin layer chromatographys (TLCs) were performed on Merck Kieselgel 60 F254. The high-performance liquid chromatography (HPLC) system consisted of a Model 306 pump, a Model 117 UV detector, a Model 234 autoinjector, and a Model 805 manometric module from Gilson (Middleton, WI, USA). A symmetry column C18 (Waters, Milford, MA, USA) (250×4.6 mm) with a guard column (Waters, 3.9×20 mm) was used. Six-week-old male Sprague Dawley rats (Samtako Bio Korea, Kyeong-gi-do, South Korea) were housed in the university animal facility with controlled temperature, humidity, and dark/light cycle. The animal protocol used in this study has been reviewed and approved by the Pusan National University-Institutional Animal Care and Use Committee (PNU-IACUC) on their ethical procedures and scientific care.
Model 234 autoinjector
Manufactured by Gilson
Sourced in United States
The Gilson Model 234 autoinjector is a versatile laboratory instrument designed for automated sample injection. It can be used to deliver precise volumes of liquid samples into analytical instruments, such as chromatography systems or spectrophotometers, with high accuracy and reproducibility.
Automatically generated - may contain errors
Lab products found in correlation
Model 306 pump, by Gilson (5 mentions)
Guard column, by Waters Corporation (4 mentions)
Symmetry c18 column, by Waters Corporation (4 mentions)
Model 805 manometric module, by Gilson (3 mentions)
Symmetry r18 column, by Waters Corporation (2 mentions)
151 variable uv detector, by Gilson (2 mentions)
3 5 dinitrosalicylic acid, by Merck Group (1 mentions)
Kieselgel 60 f254, by Merck Group (1 mentions)
Succinic anhydride, by Tokyo Chemical Industry (1 mentions)
Celecoxib, by Pfizer (1 mentions)
Inertsustain aq c18, by GL Sciences (1 mentions)
L 7400 uv detector, by Hitachi (1 mentions)
F 1050 fluorescence spectrophotometer, by Hitachi (1 mentions)
L 7100 pump, by Hitachi (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
8 protocols using model 234 autoinjector
1
Dextran-based Celecoxib Conjugate Synthesis
2
HPLC Analysis of Flavin Cofactors
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Flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), and riboflavin (RF) in the perfusate of HMP were measured using a high-performance liquid chromatography (HPLC) method. The sample (perfusate) was deproteinized by mixing it with pure methanol, followed by centrifugation and filtration. An aliquot of the resulting deproteinized supernatant was directly injected into the HPLC system. The HPLC system consisted of a GASTORR BG-42 degasser (FLOM Co., Tokyo, Japan), L-7100 pump (Hitachi High-Tech Corporation, Tokyo, Japan), model 234 autoinjector (GILSON Inc., Middleton, WI, USA), ATC-10 column oven (Eicom, Kyoto, Japan), L-7400 UV detector (Hitachi), NOD-10 UV detector (Eicom), and F-1050 fluorescence spectrophotometer (Hitachi) with C18 column for reverse phase HPLC analysis InertSustain AQ-C18 (5 µm) and a guard column E (GL Science, Tokyo, Japan). The HPLC conditions were as follows: ODS column (EICOMPAK SC5-ODS; 3 µm, 150 × 4.6 mm), column oven (40 °C), UV–Vis detector (254 nm), fluorescence detector (excitation 445 nm, emission 530 nm), mobile phase (A. methanol, B. acetic acid buffer. A/B = 35/65, v/v), where the acetic acid buffer was a mixture of 4 M sodium acetate (20 mL) and 50% acetic acid (10 mL) in 1 L of deionized water; flow (0.7 mL/min), and injection volume (25 µL). FMN, FAD, and RF contents were expressed as mmol/L.
3
HPLC Analysis of Celecoxib and Metabolites
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The HPLC system consisted of a model 306 pump, a 117 variable ultraviolet detector, a model 234 autoinjector, and a Model 805 manometric module from Gilson Inc. (Middleton, WI, USA). A C18 symmetry column (Waters, Milford, MA, USA) (250 × 4.6 mm) with a guard column (Waters, 3.9 × 20 mm) was used. The samples were filtered through a membrane filter (0.45 μm), and the filtrate (20 μL) was injected on a symmetry C18 column (Waters), which was eluted with a mobile phase at a flow rate of 1 mL/min. The mobile phase consisted of 60% ACN (Merck, Darmstadt, German) in 0.067 M phosphate buffer (pH 4.5) containing 0.1% trifluoroacetic acid (Sigma-Aldrich), which was filtered through a 0.45 μm membrane filter (Waters) before use. The eluate was monitored at 273 nm, and the detection limit was about 0.2 μg/mL under our experimental conditions. Accuracy and relative standard deviations were 98.7% and 0.43%, respectively Trilution® LC V4 software (Gilson, Middleton, WI, USA) was used for data analysis. The retention times of celecoxib, A1C, G1C, and N-GA1C were 10.68, 3.11, 3.00, and 2.43 min, respectively.
4
HPLC Analysis of 5-ASA and MCP
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The HPLC system consisted of a model 306 pump, a 151 variable UV detector, and a model 234 autoinjector from Gilson (Middleton, WI, USA). We used a Symmetry R18 column (250×4.6 mm, 5 μm; Waters, Milford, MA, USA) with a guard column (20×4.6 mm; Waters). The samples prepared from each experiment were filtered using a 0.45 μm membrane filter. HPLC analysis was conducted at a flow rate of 1 mL/min using mobile phases consisting of acetonitrile and 3.0 mM pH 3.0 phosphate buffer (2:8, v/v) for MCP and acetonitrile, and 5.0 mM pH 7.4 phosphate buffer (2:8, v/v) for 5-ASA. The eluate was monitored at 323 nm (for 5-ASA) and 275 nm (for MCP) using a UV detector measuring the absorption with a sensitivity of 0.01 absorbance units full scale. The retention times of 5-ASA and MCP were 7.5 and 9.4 minutes, respectively.
5
Quantitative HPLC Analysis of 5-ASA and ASP
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The HPLC system consisting of a model 306 pump, a 151 variable UV detector, and a model 234 autoinjector from Gilson (Middleton, WI, USA) was used for experiments. A Symmetry R18 column (250 × 4.6 mm, 5 μm) and a guard column (20 × 4.6 mm, 5 μm) were purchased from Waters (Milford, MA, USA). Samples prepared for HPLC analysis were filtered through a syringe filter (0.45 μm). HPLC analysis was performed at a flow rate of 1 mL/min using a mobile phase comprising acetonitrile and 10 mM pH 4.0 phosphate buffer (1.5:8.5, v/v). The eluate was monitored at 323 nm (for 5-ASA) and 225 nm (for ASP) with the UV detector, which measured the absorption with a sensitivity of AUFS 0.01. The retention times of 5-ASA and ASP were 7.1 and 5.5 min, respectively.
6
HPLC Analysis of Phenolic Compounds
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For high-performance liquid chromatography (HPLC) analysis, a Gilson HPLC system (Gilson, Middleton, WI, USA) was used. Samples (20 μL) filtrated through membrane filters (0.45 μm, Revodix, Gyeonggi-do, South Korea) were applied to a symmetric C18 column (Hector, Theale, Berkshire, UK; 250 × 4.6 mm, 5 μm) using a model 234 auto-injector (Gilson). tCA, tCA-GA, and tCA-AA were separated at a flow rate of 1.0 mL/min in a mobile phase comprising methanol, acetonitrile, and 1% acetic acid solution (1.5:2.2:6.3), which was monitored at 277 nm using a 151 variable UV detector (Gilson). The retention times of tCA, tCA-GA, and tCA-AA were 8.1, 17.3, and 18.5 min, respectively.
7
HPLC Analysis of MTDZ Compound
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The HPLC system consisted of Model 305 and 306 pumps, a 117 variable UV detector, a Model 234 autoinjector, a Model 805 manometric module, and a Model 811C dynamic mixer from Gilson, Inc. (Middleton, WI, USA). The mobile phase consisted of 10% acetonitrile in 0.067 M, pH 4.5 phosphate buffer, passed through a 0.45-µm membrane filter before use. A symmetry C18 column (250×4.6; Waters [Milford, MA, USA]) was eluted with the mobile phase at a flow rate of 1 mL/min. The column eluent was monitored at 319 nm with a sensitivity of 0.01 absorbance units full scale. For the analysis of MTDZ by UV spectrophotometry, a standard calibration curve was constructed from the absorbance at 319 nm of standard MTDZ solutions (1–20 ppm) in a pH 6.8 isotonic phosphate buffer.
8
HPLC Analysis of Pharmaceutical Compounds
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The high-performance liquid chromatography (HPLC) system consisted of a Gilson model 306 pump, 151 variable UV detector, and model 234 autoinjector (Gilson, Middleton, WI, USA). Chromatographic separation was conducted using a symmetric C18 column (Hector, Theale, Berkshire, UK; 250 × 4.6 mm, 5 μm). Before injection for HPLC analysis, samples were filtered through membrane filters (0.45 μm, Revodix Inc., Gyeong-gi-do, Hanam, Korea). Mobile phases were prepared as follows: mobile phase A consisted of distilled water and acetonitrile (7:3, v/v), and mobile phase B consisted of acetonitrile and 1 mM phosphate buffer (pH 7.4) with 0.5 mM tetrabutylammonium chloride (1.5:8.5, v/v). The HPLC analysis was conducted at a flow rate of 1 mL/min. The eluate was monitored at 295 nm (for DpS), 367 nm (for AS-DpS-AS) and 330 nm (for 5-ASA) using a UV detector that measured the absorption with a sensitivity of AUFS 0.01. The retention times of DpS and AS-DpS-AS using mobile phase A were 9.8 min and 7.1 min, respectively, and that of 5-ASA using mobile phase B was 10.1 min.
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