Hemavet 950fs

Manufactured by Drew Scientific

Sourced in United States, United Kingdom

The Hemavet 950FS is a compact and automated hematology analyzer designed for veterinary use. It provides a comprehensive analysis of complete blood count (CBC) parameters, including red blood cells, white blood cells, and platelet counts, as well as related indices. The device uses advanced technology to deliver accurate and reliable results quickly.

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Lab products found in correlation

Edta coated microvette tubes, by Sarstedt (15 mentions) Ethylene diamine tetraacetic acid (edta), by BD (4 mentions) Edta and silicone coated tubes, by Sarstedt (3 mentions) Z serum clot activator vacuette tube, by Greiner (3 mentions) Microtainer tube, by BD (3 mentions) Lsrii instrument, by BD (2 mentions) Facscalibur, by BD (2 mentions) Catalyst dx, by IDEXX (2 mentions) Mep00b, by RnD Systems (2 mentions) Acid citrate dextrose, by Merck Group (2 mentions) Edta tube, by BD (2 mentions) Piccolo point of care analyzer, by Abaxis (2 mentions) General chemistry 13 panel, by Abaxis (2 mentions) K2edta coated microtainer tubes, by BD (2 mentions) Sodium citrate, by Greiner (2 mentions) Microtainer 365974, by BD (2 mentions) Alamarblue assay, by Thermo Fisher Scientific (2 mentions) Polyi polyc pi pc, by GE Healthcare (2 mentions) Microhematocrit capillary tube, by Thermo Fisher Scientific (2 mentions) Lithium heparin coated microvette tubes, by Sarstedt (1 mentions)

Spelling variants of this product

Hemavet 950FS analyzer (11 citations) HEMAVET® 950FS Hematology System (9 citations) Hemavet 950FS Multi-species Hematology System (7 citations) Hemavet 950FS Veterinary Multi-Species Hematology System (4 citations) Hemavet 950FS analyser (3 citations) Hemavet 950FS blood cell analyzer (3 citations) Hemavet 950F hematology analyzer (2 citations) Hemavet 950FS whole blood counter (2 citations) MASCOT HEMAVET 950FS (2 citations) Hemavet 950FS blood analyzer (2 citations)

205 protocols using hemavet 950fs

Blood Collection and Analysis

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PB from mice was retro-orbitally collected into lithium heparin-coated Microvette tubes (Sarstedt), and the tubes were gently rotated for 10 min. Complete blood cell counts were analyzed using a Hemavet 950FS (Drew Scientific) according to the manufacturer’s instructions.

Tanaka-Yano M., Zong L., Park B., Yanai H., Tekin-Turhan F., Blackshear P.J, & Beerman I. (2023). Tristetraprolin overexpression drives hematopoietic changes in young and middle-aged mice generating dominant mitigating effects on induced inflammation in murine models. GeroScience, 46(1), 1271-1284.

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Whole Blood Collection and CBC Analysis

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Whole blood was collected via cardiac puncture using a 1 mL BD Slip-Tip Disposable Tuberculin Syringe (28-gauge; BD Biosciences) and mixed with a 0.5 M EDTA pH 8.0 solution (1:10 ratio; AmericanBio, Natick, MA, USA). CBC analysis, including the number of white blood cells, and percentage of lymphocyte, monocyte, neutrophil, eosinophil, and basophil was measured using a Hemavet 950FS (Drew Scientific, Dallas, TX, USA) according to the manufacturer’s instructions.

Kwon H.K., Dussik C.M., Kim S.H., Kyriakides T.R., Oh I, & Lee F.Y. (2022). Treating ‘Septic’ With Enhanced Antibiotics and ‘Arthritis’ by Mitigation of Excessive Inflammation. Frontiers in Cellular and Infection Microbiology, 12, 897291.

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Murine Blood and Bone Marrow Analysis

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Peripheral blood was collected from mice via submandibular bleeding and blood counts were recorded using the Hemavet 950fs (Drew Scientific). Blood counts were tabulated graphically with error bars corresponding to standard error of the means and compared using 2-tailed ttests. Bone marrow cellularity was determined using hemocytometer under light microscope.
Total marrow was incubated with lineage markers (CD3, CD4, B220, Ter119, CD11b) and Sca1+/c-Kit+ cells in lineage negative cells determined via flow cytometry on a BD LSRII instrument (BD Biosciences). Data was analyzed on FlowJo software (Treestar). Values were tabulated graphically with error bars corresponding to standard error of the means and compared using 2-tailed t-tests.

Ho W.J., Smith J., Park Y.S., Hadiono M., Christo K.F., Jogasuria A., Zhang Y., Broncano A.V., Kasturi L., Dawson D., Gerson S.L., Markowitz S.D., & Desai A.B. (2020). 15-PGDH Regulates Hematopoietic and Gastrointestinal Fitness During Aging.

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White Blood Cell Quantification

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To measure the number of white blood cells in blood upon challenge, 200 μl of blood were collected into BD Microtainer^® Blood Collection Tubes (BD Biosciences). Blood samples were analyzed immediately using an Hemavet 950 FS (Drew Scientific). The results were compared to a mouse blood standard (Drew Scientific).

Sen-Kilic E., Blackwood C.B., Boehm D.T., Witt W.T., Malkowski A.C., Bevere J.R., Wong T.Y., Hall J.M., Bradford S.D., Varney M.E., Damron F.H, & Barbier M. (2019). Intranasal Peptide-Based FpvA-KLH Conjugate Vaccine Protects Mice From Pseudomonas aeruginosa Acute Murine Pneumonia. Frontiers in Immunology, 10, 2497.

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Submandibular Blood Collection for Hematology

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Blood was collected by submandibular vein puncture into Microtainer EDTA tubes (BD Biosciences) for hematologic analysis (Hemavet 950 FS; Drew Scientific, Inc., Dallas, TX) or Microtainer serum separator tubes (BD Biosciences) for serum chemistry analysis on a Liasys 330 AMS Diagnostic liquid chemistry analyzer (Weston, FL). Investigators were blinded to treatment groups during analysis.

Ross M.H., Esser A.K., Fox G.C., Schmieder A.H., Yang X., Hu G., Pan D., Su X., Xu Y., Novack D.V., Walsh T., Colditz G.A., Lukaszewicz G.H., Cordell E., Novack J., Fitzpatrick J.A., Waning D.L., Mohammad K.S., Guise T.A., Lanza G.M, & Weilbaecher K.N. (2017). Bone-induced expression of integrin β3 enables targeted nanotherapy of breast cancer metastases. Cancer research, 77(22), 6299-6312.

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Monitoring Animal Health in Research

Cited 1 time

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All live animals were monitored daily for clinical signs under anesthesia twice a day. Body temperature was measured by inserting a soft probe attached to an Omron electronic digital stick thermometer (MC-BOMR, Omron Co.) into the rectum 2 cm from the anal margin for one minute [18] (link). All clinical signs were monitored from day 2 p.i.. Venous blood of biological indicators such as blood cell count using the Veterinary Multi-species Hematology System (Hemavet 950FS, Drew Scientific Co.) and evaluation of the viral load were performed.

Zhang Y., Yang E., Pu J., Liu L., Che Y., Wang J., Liao Y., Wang L., Ding D., Zhao T., Ma N., Song M., Wang X., Shen D., Tang D., Huang H., Zhang Z., Chen D., Feng M, & Li Q. (2014). The Gene Expression Profile of Peripheral Blood Mononuclear Cells from EV71-Infected Rhesus Infants and the Significance in Viral Pathogenesis. PLoS ONE, 9(1), e83766.

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Postoperative Blood Analysis Protocol

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At time points one and five weeks post-surgery, blood was collected into EDTA-coated tubes for complete blood count analysis (CBC) performed using a Hemavet 950FS (Drew Scientific Inc., Waterbury, CT). Plasma was prepared for thrombin clotting time (TT), thrombin activity assay, and plasma inflammatory biomarkers. The TT was determined by Hemoclot® thrombin inhibitor assay, according to the manufacturer’s protocol (ANIARA, Mason, OH). The diluted TT is particularly sensitive to the effects of dabigatran, and displays a linear dose-response over therapeutic concentrations; therefore, dabigatran plasma concentrationwas calculated from diluted TT (26 (link)). Plasma thrombin activity was detected using SensoLyte® 520 thrombin activity assay kit, according to the manufacturer’s protocol (AnaSpec, Fremont, CA). Plasma cytokines were quantified using a mouse 96-well multiplex assay kit (MPXMCYTO-70K; Millipore, Billerica, MA), according to the manufacturer’s protocol. Bead fluorescence was detected using a BioRad Bioplex 200 suspension array reader and analyzed using Bio-Plex Manager 4.0 software (BioRad Laboratories, Hercules, CA).

Dong A., Mueller P., Yang F., Yang L., Morris A, & Smyth S.S. (2017). Direct thrombin inhibition with dabigatran attenuates pressure overload-induced cardiac fibrosis and dysfunction in mice. Thrombosis research, 159, 58-64.

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Porcine Hematological Response Assessment

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The pig BW and feed intake were recorded at the starting day and end of each
phase. Average daily gain (ADG), average daily feed intake (ADFI) and gain to
feed ratio (G:F) were calculated. Blood samples were collected from the jugular
vein of 8 pigs per treatment using 8 mL EDTA tubes. The blood samples were
analyzed to measure number of white blood cells and proportions of their
differentiation using a multi-parameter automated hematology analyzer calibrated
for porcine blood (Hemavet 950FS, Drew Scientific, UK).

Min Y., Choi Y., Kim Y., Jeong Y., Kim D., Kim J., Jung H, & Song M. (2019). Effects of protease supplementation on growth performance, blood constituents, and carcass characteristics of growing-finishing pigs. Journal of Animal Science and Technology, 61(4), 234-238.

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Blood Characterization in Mice

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Blood was obtained from euthanized mice by cardiac puncture using 0.2M EDTA as an anticoagulant. Complete blood cell counting was done on an aliquot using a Drew Scientific Hemavet 950FS (Miami Lakes, FL. USA). Plasma was isolated from the remaining blood and aliquots were used to measure insulin by ELISA (Millipore; Burlington, MA. USA), and total plasma cholesterol, HDL, low density lipoprotein (LDL), and triglycerides using an Axcel Clinical Chemistry Analyzer (West Caldwell, NJ. USA). HOMA IR - scores were calculated as described (23 (link)).

Zhao J., Gomes D., Jin L., Mathis S.P., Li X., Rouchka E.C., Bodduluri H., Conklin D.J, & O’Toole T.E. (2022). Polystyrene bead ingestion promotes adiposity and cardiometabolic disease in mice. Ecotoxicology and environmental safety, 232, 113239.

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Facial Vein Blood Collection and Analysis

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Peripheral blood was obtained from the facial vein via submandibular bleeding, collected in an ethylenediaminetetraacetic acid (EDTA) tube, and analyzed using a Hemavet 950FS (Drew Scientific).

Castiglione M., Jiang Y., Mazzeo C., Lee S., Chen J., Kaushansky K., Yin W., Lin R.Z., Zheng H, & Zhan H. (2020). Endothelial JAK2V617F mutation leads to thrombosis, vasculopathy, and cardiomyopathy in a murine model of myeloproliferative neoplasm. Journal of Thrombosis and Haemostasis, 18(12), 3359-3370.

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