Caco-2 cells, a human epithelial colorectal adenocarcinoma cell line, were maintained in α-minimal essential medium (Sigma, St. Louis, MO), supplemented with 15% fetal bovine serum (HyClone Labs, Logan, UT), 100 units/mL penicillin, and 100 μg/mL streptomycin (Sigma) in a humidified incubator at 37°C, with an atmospheric composition of 95% air and 5% CO2. In treatment experiments cell culture medium contained 10% delipidated serum. Lipid was removed from serum by incubation with fumed silica powder (0.007 µm) as described by the manufacturer (Sigma, St. Louis, MO). Cells were exposed to Lovastatin (10 µM), BNF (10 µM), TCDD (10 nM), SGA360 (10 µM), SGA315 (10 µM) or carrier solution dimethyl sulfoxide (DMSO) for indicated time points. Enriched normal primary human hepatocytes were obtained through the Liver Tissue Cell Distribution System (University of Pittsburgh, PA). The isolated hepatocytes were seeded at ~90% confluence in 24-well collagen-coated dishes and cultured as previously described.20
α minimal essential medium
Manufactured by Merck Group
Sourced in United States, United Kingdom
α-minimal essential medium is a cell culture medium that provides the basic nutrients and supplements required for the in vitro growth and maintenance of cells. It serves as a core component in various cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Merck Group (6 mentions)
Penicillin, by Merck Group (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Fumed silica powder, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Cytiva (2 mentions)
Hepa 1 and caco2 cells, by American Type Culture Collection (2 mentions)
Roswell park memorial institute (rpmi), by Merck Group (2 mentions)
Foetal bovine serum (fbs), by Merck Group (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions)
Ls174t cells, by American Type Culture Collection (1 mentions)
Caco 2, by American Type Culture Collection (1 mentions)
Fetal calf serum (fcs), by Harvard Bioscience (1 mentions)
Triptolide, by Santa Cruz Biotechnology (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Plasmocin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Penicillin g, by Merck Group (1 mentions)
15 protocols using α minimal essential medium
1
Caco-2 Cell Culture and Compound Treatments
2
Culturing Primary Human Mesenchymal Stem Cells
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Primary hMSC were purchased from Lonza (Slough, UK) and cultured in α-Minimal Essential Medium (MEM), penicillin (50 U/ml), streptomycin (50 μg/ml) (all from Sigma–Aldrich, Poole, Dorset, UK), Glutamax (2 mM) (Invitrogen, Paisley, UK) and 10% foetal bovine serum (FBS) (Sigma–Aldrich) and maintained at 37 °C in a humidified 5% CO2: 95% air atmosphere.
3
Cell Culture Protocols for Reporter Assays
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The HepG2 40/6 stable DRE-driven reporter line was generated and cultured, as previously described [15 (link)]. The Hepa 1.1 stable DRE-driven reporter cell line was obtained from Dr. Michael Denison (University of California, Davis, CA, USA) and cultured under the same conditions as HepG2 40/6 cells. The human epithelial colorectal adenocarcinoma cell line Caco-2 was obtained from ATCC and maintained in α-minimal essential medium (Sigma, Hong Kong, China; M0894), supplemented with 20% fetal bovine serum (Gemini), 100 U/mL penicillin, and 100 μg/mL streptomycin; for actual treatments, the medium was replaced by a medium containing 5% serum. HN30 cells were obtained and cultured, as previously described [16 (link)]. LS174T cells (ATCC #3521130) were cultured with α-minimal essential medium supplemented with 8% (v/v) fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin. All cells were grown in a humidified incubator at 37 °C, with 95% air and 5% CO2.
4
Isolation of Dental Stem Cells
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Dental follicles (n = 4) and healthy normal human third molars were collected from patients (14–21 years old) at the Ziekenhuis Maas and Kempen, Bree and ZOL Genk with written informed consent and approved by the medical ethical committee of Hasselt University (protocol 13/0104U). The apical papillae (n = 5) and dental pulp (n = 6) were separated from the teeth and all tissues were collected in α-Minimal Essential Medium (Sigma-Aldrich, Overijse, Belgium) supplemented with 10% heat inactivated fetal calf serum (FCS) (Biochrom AG, Berlin, Germany), 2 mM L-Glutamine, 100 U/ml Penicillin and 100 μg/ml Streptomycin (Sigma-Aldrich). Stem cells were isolated via the explant method as described previously (Hilkens et al., 2013 (link)). Briefly, pieces of 1 mm3 were placed into a 6-well plate containing culture medium. Explants were cultured for 14 days allowing stem cells to grow out of the tissue at 37°C in a humidified atmosphere containing 5% CO2. Medium was changed twice a week. After 10 to 14 days, 80% to 90% confluency was reached and cells were sub-cultured. For all experiments, cells of passage one to three were used.
5
Cell Culture Maintenance Protocol
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Hepa 1 and Caco2 cells were obtained from American Type Culture Collection and maintained in α-minimal essential medium (Sigma-Aldrich, St. Louis, MO) supplemented with 10% or 15% fetal bovine serum (Hyclone Laboratories, Logan, UT), respectively, and 100 U/mL penicillin and 100 µg/mL streptomycin. Cells were maintained at 37°C in a humidified incubator in 95% air and 5% CO2.
6
Triptolide Inhibits Breast Cancer Cell Lines
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Triptolide (molecular formula, C20H24O6; molecular weight, 360.4 g/mol) was purchased from Santa Cruz Biotechnology, Inc. (sc-200122; Santa Cruz, CA, USA). Triptolide was dissolved in dimethyl sulfoxide (DMSO). Three breast cancer cell lines, specifically, the highly metastatic MDA-MB-231, HER2-positive BT-474 and ER-positive MCF7 cell lines, were provided by the Department of Oncology at the Hospital of Traditional Chinese Medicine (Yantai, China). MDA-MB-231, BT-474 and MCF-7 cells were cultured in Dulbecco’s modified Eagle’s medium, RPMI and α-minimal essential medium (Sigma-Aldrich, St. Loius, MO, USA), respectively. Cells were cultured at 37°C with 5% CO2 and 100% humidity. The medium was supplemented with 10% fetal bovine serum (FBS; HyClone Laboratories, Inc., Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
7
Klf10 Overexpression in iMDP-3 Cells
8
Caco-2 Cell Culture and Compound Treatments
9
Isolation and Expansion of Adipose-Derived Cells
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Subcutaneous adipose tissue from donor animals was excised from the ventral neck area and kept at 4°C in phosphate‐buffered saline (Sigma‐Aldrich, St. Louis, MO) supplemented with 1% penicillin/streptomycin (Gibco, Grand Island, NY). Cells were isolated and cultured as described previously.11 , 15 The cells were expanded in α‐minimal essential medium (Sigma‐Aldrich) supplemented with 10% fetal bovine serum, 1 mmol/L l ‐glutamine, and 1% penicillin/streptomycin (Gibco), and 5 μg/mL Plasmocin™ (Invitrogen) under standard culture conditions (37°C, 5% CO2), with medium replacement every 3 days. Third‐passage cells were labeled by transduction (2×106 transducing units/mL multiplicity of infection=21, 48 hours) with a lentiviral vector containing enhanced green fluorescent protein (GFP) under constitutive transcriptional control.16 Cells expressing GFP were selected by fluorescence‐activated cell sorting and frozen before in vivo administration.
10
Isolation and Culture of Gingival Fibroblasts
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GF were obtained from tooth donations with oral and written informed consent of the patient at the University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria with approval of the Ethics Committee of the Medical University of Vienna (631/2007). Gingival tissue was removed from uninflamed teeth and cultured in petri dishes containing cell culture medium (α-minimal essential medium; Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% fetal bovine serum (Gibco, Thermo FischerScientific, MA, USA), penicillin, streptomycin and amphotericin (Gibco, Thermo Fischer). The heterogeneous cell population that grew out from the tissue and consists mainly of fibroblasts was further cultured as GF at 37 °C, 5% CO2 and 95% humidity. For experiments, cell passages 4–8 were used. Cells in all cultures and experiments were conducted in α-minimal essential medium with 10% fetal bovine serum and antibiotics and kept at 37 °C, 5% CO2 and 95% humidity.
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