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Celltiter glo kit

Manufactured by Promega
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Sourced in United States, Switzerland, Germany

The CellTiter-Glo kit is a quantitative, homogeneous assay that measures ATP, which indicates the presence of metabolically active cells. The kit utilizes a proprietary thermostable luciferase to generate a luminescent signal that is proportional to the amount of ATP present.

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Lab products found in correlation

Synergy microplate reader, by Agilent Technologies (11 mentions) Glomax 96 microplate luminometer, by Promega (6 mentions) Prism software, by GraphPad (5 mentions) Infinite 2000 pro reader, by Tecan (4 mentions) Envision plate reader, by PerkinElmer (3 mentions) White 96 well clear bottomed plates, by Corning (3 mentions) M200 pro infinity plate reader, by Tecan (3 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (3 mentions) Glomax 96, by Promega (2 mentions) Spectrafluor plus plate reader, by Tecan (2 mentions) Spectramax m5, by Molecular Devices (2 mentions) Infinite 200 pro plate reader, by Tecan (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Synergy ht microplate reader, by Agilent Technologies (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Il 1β, by Santa Cruz Biotechnology (2 mentions) Nlrp3, by R&D Systems (2 mentions) Mouse caspase 1, by Adipogen (2 mentions) Adenosine triphosphate (atp), by Merck Group (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions)

240 protocols using celltiter glo kit

1

Cell Viability Assay using CellTiter-Glo

Cited 2 times
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Cell viability was determined by using the CellTiter-Glo kit (Promega) as described previously (37 (link), 59 (link)). Briefly, HEK293T cells seeded in opaque-walled 96-well plates were transfected with siRNA targeting ANP32A or with AllStars negative-control siRNA at a concentration of 30 nM. At 48 h posttransfection, luminescence was measured with the CellTiter-Glo kit (Promega).
The cell viability assay was similarly performed for the Anp32a-KO HEK293T cells, Anp32a-KO A549 cells, and the corresponding control cells grown in opaque-walled 96-well plates.
Liang L., Jiang L., Li J., Zhao Q., Wang J., He X., Huang S., Wang Q., Zhao Y., Wang G., Sun N., Deng G., Shi J., Tian G., Zeng X., Jiang Y., Liu L., Liu J., Chen P., Bu Z., Kawaoka Y., Chen H, & Li C. (2019). Low Polymerase Activity Attributed to PA Drives the Acquisition of the PB2 E627K Mutation of H7N9 Avian Influenza Virus in Mammals. mBio, 10(3), e01162-19.
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2

Cell Viability Assay of Deltasonamide 2 and FPFT-2216

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800 cells were seeded in 384 well plate (Corning, #3570) with a total volume of 50 μL. Next day, cells were treated with Deltasonamide 2 (MedChemExpress, #HY-122641A) or FPFT-2216 at indicated concentrations/frequency. After three-day treatment, cell viability was measured using CellTiter-Glo kits (Promega, #G7572) according to manufacturer instructions. After 20 min incubation, luminescence signal was collected using EnVision plate reader (PerkinElmer).
Raimondi F., Kao J.P., Fiorentini C., Fabbri A., Donelli G., Gasparini N., Rubino A, & Fasano A. (2000). Enterotoxicity and Cytotoxicity of Vibrio parahaemolyticus Thermostable Direct Hemolysin in In Vitro Systems. Infection and Immunity, 68(6), 3180-3185.
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3

High-Throughput Screening of Cytotoxic Agents

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High-throughput drug screening (HDS) was used to evaluate the cytotoxic effect of different candidate agents on NRASG12-transformed Ba/F3 cells (Supplementary Figure S1). Transformed Ba/F3 cells were grown in RPMI160 supplemented with 10% FBS and seeded in a 384-well plate (Corning, NY, United States) at a density of 1200 cells per well. The initial concentration of targeting drugs (Supplementary Table S4) was 10 μM and then serial diluted to generate the drug concentration series (10, 3.3, 1.1, 0.37, 0.12, 0.04, 0.013, 0.0045, 0.0015, and 0.0005 μM). The serial drug concentrations were added to the cells using an automated liquid handling system (PerkinElmer, MA, United States). Cell viability was assessed using CellTiter-Glo™ kits (Promega, WI, United States) after 72 h of drug exposure. The inhibition rate of each drug concentration was calculated after normalization using the formula below. The IC50 was calculated using GraphPad Prism v7.0 (GraphPad Software, Inc.). The HDS experiments were performed in triplicate and independently repeated three times. Inhibition rate (%)=100%−RLUDrug−RLUBackgroundRLUDMSO−RLUBackground×100%
Qian J., Li Z., Pei K., Li Z., Li C., Yan M., Qian M., Song Y., Zhang H, & He Y. (2022). Effects of NRAS Mutations on Leukemogenesis and Targeting of Children With Acute Lymphoblastic Leukemia. Frontiers in Cell and Developmental Biology, 10, 712484.
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4

Cell Viability Assay in 96-Well Plates

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1500 cells were seeded per well into 96-well plates in triplicates. Cell viability was determined using the Cell Counting Kit-8 (CCK-8, Dojindo) or CellTiter-Glo kits (Promega) according to manufacturer's instructions. Absorbance at 450 nm was determined using a Varioscan system (Thermo Fisher).
Du Y., Rokavec M, & Hermeking H. (2023). Squalene epoxidase/SQLE is a candidate target for treatment of colorectal cancers with p53 mutation and elevated c-MYC expression. International Journal of Biological Sciences, 19(13), 4103-4122.
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5

Cell Viability Assay with CellTiter Glo

Cited 1 time
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Viability assays were performed using CellTiter Glo kits (Promega) as previously described [49 (link)]. Luminescence intensity was measured using an Infinite 2000 Pro reader (Tecan).
Bi X., Braunstein M., Shei G.J, & Broach J.R. (1999). The yeast HML I silencer defines a heterochromatin domain boundary by directional establishment of silencing. Proceedings of the National Academy of Sciences of the United States of America, 96(21), 11934-11939.
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6

ATP Secretion Profiling of MDA-MB-231 Cells

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The MDA-MB-231 cells were cultured in 60 mm culture dishes in the presence of vehicle (<0.1% DMSO), TPH104 (1 or 5 µM), and MX (1 µM). The supernatants were collected after 0, 24 and 48 h of incubation and ATP levels in the supernatants were measured using CellTiter-Glo® kits (Promega, Madison, WI, USA). The supernatants collected were concentrated by centrifugation in 10,000 MWCO (molecular weight cutoff) centrifuge tubes (Millipore Sigma, Burlington, MA, USA) at 3000× g for 30 min at 4 °C and stored at −80 °C. VC-CM represents the vehicle control (VC) conditioned media (CM) of MDA-MB-231 cells incubated with vehicle, whereas TPH104-1-CM describes the CM of MDA-MB-231 cells incubated with TPH104 (1 µM); TPH104-5-CM, the CM of MDA-MB-231 cells incubated with TPH104 (5 µM); and MX-1-CM, the CM of MDA-MB-231 cells incubated with MX (1 µM). A schematic representation of the protocol is provided in Supplementary Figure S1.
Tukaramrao D.B., Malla S., Saraiya S., Hanely R.A., Ray A., Kumari S., Raman D, & Tiwari A.K. (2021). A Novel Thienopyrimidine Analog, TPH104, Mediates Immunogenic Cell Death in Triple-Negative Breast Cancer Cells. Cancers, 13(8), 1954.
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7

ATP-Based Cell Viability Assay

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Cell viability was determined by measuring the level of intracellular ATP with the CellTiter-Glo kit (Promega, Madison, WI) according to the manufacturer's instructions. Briefly, A549 cells seeded into opaque-walled 96-well plates were transfected with siRNA targeting TRAPPC6AΔ or with nontargeting siRNA at a concentration of 40 nM. At 48 h posttransfection, 100 μl of CellTiter-Glo reagent was added directly into each well, and the contents were mixed for 2 min on a shaker to induce cell lysis. After a 10-min incubation at room temperature, luminescence was recorded with a GloMax 96 microplate luminometer (Promega).
Zhu P., Liang L., Shao X., Luo W., Jiang S., Zhao Q., Sun N., Zhao Y., Li J., Wang J., Zhou Y., Zhang J., Wang G., Jiang L., Chen H, & Li C. (2016). Host Cellular Protein TRAPPC6AΔ Interacts with Influenza A Virus M2 Protein and Regulates Viral Propagation by Modulating M2 Trafficking. Journal of Virology, 91(1), e01757-16.
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8

Cytotoxicity Assay on MRC-5 Cells

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The synthesized compounds were assayed for cytotoxicity on MRC‐5 (human foetal lung fibroblasts) cells. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 25 mm glucose, 10% (v/v) serum, 1% penicillin/streptomycin and kept under 5% CO2 at 37 °C. The cell viability was assessed using the CellTiter Glo kit from Promega (G7572) after 72‐h incubation time using four concentrations of compounds (1, 10, 25 and 50 μm) in triplicate.
Rahimova R., Nogaret P., Huteau V., Gelin M., Clément D.A., Labesse G., Pochet S., Blanc‐Potard A, & Lionne C. (2022). Structure‐based design, synthesis and biological evaluation of a NAD + analogue targeting Pseudomonas aeruginosa NAD kinase. The Febs Journal, 290(2), 482-501.
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9

Cell Viability Assay for HepG2, MCF7, and MCF-10A

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Cell lines HepG2 and MCF7 were acquired from National Center for Cell Science (Pune, Maharashtra, India) and MCF-10A was obtained from Dr. Carlos L. Arteaga at Southwestern Medical Center. Cells were grown in DMEM supplemented with 10% fetal bovine serum in a humidified CO2 incubator at 37 °C. All cell culture reagents were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA).
Approx. 15,000 cells were plated in each well of transparent flat bottom 96 well plate. Treatment of win, with respective dosages, was started after cells attached overnight and continued for 72 h. At the end of experiment viability was measured either using Cell Titer Glo kit (Promega Corporation, Madison, Wisconsin, USA) as per manufacturer instructions or 0.5% crystal violet staining. Absorbance was measured at 595 nm.
Siddiqui S., Ahmed N., Goswami M., Chakrabarty A, & Chowdhury G. (2021). DNA damage by Withanone as a potential cause of liver toxicity observed for herbal products of Withania somnifera (Ashwagandha). Current Research in Toxicology, 2, 72-81.
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10

Cell Proliferation and Survival Assays

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Cell proliferation was measured with a CellTiter-Glo kit according to the manufacturer’s manual (Promega, USA). About 1 × 103 cells were plated in a 96-well plate and incubated with 1 μM hHK-1 for the indicated time. To measure the drug effect on cell survival, 3 × 103 cells were plated in a 96-well plate and treated with the indicated drugs for 72 h. The luminescence was measured by Flex Station III microplate reader (Molecular Devices, Sunnyvale, Silicon Valley, USA). For colony formation assay, 800 cells were seeded into a six-well plate and incubated with hHK-1 in RPMI 1640 medium. The cells were replaced with fresh medium every 3 days for 2 weeks, then were fixed in 90% ethanol and stained with 0.1% crystal violet. The colonies >1 mm were counted by Image J.
Zhang X.W., Li J.Y., Li L., Hu W.Q., Tao Y., Gao W.Y., Ye Z.N., Jia H.Y., Wang J.N., Miao X.K., Yang W.L., Wang R, & Mou L.Y. (2023). Neurokinin-1 receptor drives PKCɑ-AURKA/N-Myc signaling to facilitate the neuroendocrine progression of prostate cancer. Cell Death & Disease, 14(6), 384.
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