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Jsm 6010plus la

Manufactured by JEOL
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Sourced in Japan

The JSM-6010PLUS/LA is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of samples. It features a LaB6 electron source, which provides improved brightness and resolution compared to conventional tungsten filament sources. The JSM-6010PLUS/LA is capable of magnifications up to 100,000x and can accommodate a variety of sample types, from solid materials to biological specimens.

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Lab products found in correlation

Em cpd300, by Leica camera (3 mentions) B2901a precision smu, by Keysight (2 mentions) Jem 1400plus, by JEOL (2 mentions) Ace200, by Leica camera (1 mentions) Cpd300, by Leica camera (1 mentions) Nano series, by Malvern Panalytical (1 mentions) Ht laser, by Malvern Panalytical (1 mentions) Zen3600, by Malvern Panalytical (1 mentions) Zetasizer, by Malvern Panalytical (1 mentions) Bx51 microscope, by Olympus (1 mentions) Ultradry eds detector, by Thermo Fisher Scientific (1 mentions) Jsm 6060lv, by JEOL (1 mentions) D2 phaser, by JEOL (1 mentions) Jec 3000fc, by JEOL (1 mentions) Image pro plus 6.0 program, by Media Cybernetics (1 mentions) Uv visible spectrophotometer, by Shimadzu (1 mentions) Leica af6000, by Leica Microsystems (1 mentions) Smartlab xe, by Rigaku (1 mentions) Miniflex 600, by Rigaku (1 mentions) Tecnai g2 spirit biotwin, by Thermo Fisher Scientific (1 mentions)

26 protocols using jsm 6010plus la

1

SEM Imaging of SiOC Scaffolds with/without Cells

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For scanning electron microscopy analysis, the SiOC scaffolds (without cells) were first coated with gold (Leica EM ACE200) and then analyzed using a JSM6010 PLUS-LA (JEOL) scanning electron microscope.
The scaffolds containing cells were first fixed (2.5% glutaraldehyde; 0.1 M sodium cacodylate buffer) for 1 hour at room temperature and stored at 4°C (in the same solution) until used. The samples were then washed (0.1 M sodium cacodylate buffer), post-fixed (1% osmium tetroxide; 0.1 M sodium cacodylate buffer) for 40 min, and washed again. The samples were dehydrated with a series of ethanol solutions with increasing concentrations (30%, 50%, 70%, 90%, and 100%), dried using a critical point (Leica EM CPD300), and coated with gold (Leica EM ACE200). The samples were analyzed using a JSM6010 PLUS-LA (JEOL) scanning electron microscope. For energy-dispersive X-ray spectroscopy (EDS), the post-fixation step with osmium tetroxide was omitted, and the samples were coated using carbon.
Carnieri M.V., Garcia D.D., Voltolini R., Volpato N., Mafra M., Bernardelli E.A., Stimamiglio M.A., Rebelatto C.K., Correa A., Berti L.F, & Marcon B.H. (2024). Cytocompatible and osteoconductive silicon oxycarbide glass scaffolds 3D printed by DLP: a potential material for bone tissue regeneration. Frontiers in Bioengineering and Biotechnology, 11, 1297327.
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2

Microstructural Analysis of Zirconia-Reinforced Filler Metal

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The cast filler samples were ground metallographically with SiC paper, polished with Al2O3 suspensions, and etched with a 10 vol. % H3PO4 solution at 50 °C for 1 minute. The phases present in the microstructure of the filler metal with different ZrO2 were examined by analytical scanning electron microscopy (ASEM, JEOL JSM-6010 PLUS/LA, Tokyo, Japan). The phase composition in filler metal were quantified using energy-dispersive spectroscopy (EDS, ASEM, JEOL JSM-6010 PLUS/LA, Tokyo, Japan) equipped with Image-Pro Plus 6.0 program (Media Cybernetics, Inc., Rockville, MD, USA). For EDS compositional analysis, the authors have analyzed approximately 50 spots for each phase and the mean value is considered. High resolution transmission electron microscopy (Philips FEI Technai G2 twin, USA) analysis was used to observe the dispersion of ZrO2 nanomaterials in the filler metal matrix.
Jung D.H., Rajendran S.H, & Jung J.P. (2018). Effect of ZrO2 Nanomaterials on Wettability and Interfacial Characteristics of Al-19Cu-11Si-2Sn Filler Metal for Low Temperature Al to Cu Dissimilar Brazing. Nanomaterials, 8(10), 784.
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3

Microscopic Analysis of PBT Composites

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The Scanning Electron Microscope (SEM) observations were made using a Jeol JSM-6010 PLUS/LA (Croissy-sur-Seine, France). SEM specimens were cut transversally from the tensile test specimens, coated with resin and then polished. The observations were made on different specimens with different rates: 0% (unreinforced PBT), 5% and 30% GF reinforced PBT, and observed under low pressure. The observations were made at different scales to better capture the damage mechanics.
Hamlaoui O., Klinkova O., Elleuch R, & Tawfiq I. (2021). Effect of the Glass Fiber Content of a Polybutylene Terephthalate Reinforced Composite Structure on Physical and Mechanical Characteristics. Polymers, 14(1), 17.
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4

Cell Preparation for Scanning Electron Microscopy

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The cells were collected by centrifugation at 5,000 rpm for three minutes and were washed 3 times with PBS buffer and then was fixed with 1 mL of 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.2 for 1 h at room temperature and subsequently they were washed 3 times with post-fixation solution (0.1 M cacodylate buffer, 0.2 M sucrose and 2 mM MgCl2). After the fixation step, the cells were adhered in round coverslips with poly-I-lysine for 30 min. The excess was quickly removed, and the samples were gradually dehydrated, by adding sequentially adding ethanol 30%, ethanol 50%, and ethanol 70% for 5 min each and 95% ethanol followed by 100% ethanol, twice, for 10 min each. After dehydration, 100% ethanol was replaced with carbon dioxide (CO2) with a 10 times cycle. At the end of the process, the coverslips were metallized with gold and visualized in a scanning electron microscope (Jeol JSM-6010 Plus-LA) at 5 Kv.
Zamith-Miranda D., Amatuzzi R.F., Munhoz da Rocha I.F., Martins S.T., Lucena A.C., Vieira A.Z., Trentin G., Almeida F., Rodrigues M.L., Nakayasu E.S., Nosanchuk J.D, & Alves L.R. (2021). Transcriptional and translational landscape of Candida auris in response to caspofungin. Computational and Structural Biotechnology Journal, 19, 5264-5277.
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5

Surface Morphology and Crystallinity Analysis

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Film samples were examined for surface morphology using SEM (JSM-6010PLUS/LA, Jeol, Tokyo, Japan). The films were prepared twice from freshly prepared solutions. Small sections from three different areas of films were cut and used for capturing image. A total 9 pictures were taken for each film with different magnifications to cover all the area. The SEM images with 500× magnification were chosen. Gold coatings were applied to the film strips. The SEM investigations were carried out at an accelerating voltage of 5 kV. The crystalline nature of the films was examined by SHIMADZU Lab X–XRD–6100 diffractometer, Shimadzu Corp., Kyoto, Japan. The XRD was performed at Cu-Kα radiation, 40 kV accelerating voltage, and 15 mA current. XRD profiles were recorded at a scan rate of 2/min and scan range from 5 to 65.
Kaur N., Somasundram C., Razali Z., Mourad A.H., Hamed F, & Ahmed Z.F. (2024). Aloe vera/Chitosan-Based Edible Film with Enhanced Antioxidant, Antimicrobial, Thermal, and Barrier Properties for Sustainable Food Preservation. Polymers, 16(2), 242.
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6

Scanning Electron Microscopy of Cells

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Control or MMV1593537-treated cells were washed 3 times with PBS and fixed with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.2) for 1 h at room temperature. The cells were washed 3 times with 0.1 M sodium cacodylate buffer (pH 7.2) containing 0.2 M sucrose and 2 mM MgCl2 and placed over 0.01% poly-l-lysine-coated coverslips. The fixed cells were allowed adhere to the coverslips for 1 h at room temperature, followed by dehydration in ethanol (30, 50, and 70% for 5 min, 90% for 10 min, and 100% twice for 10 min). Dehydrated cells were critical point-dried (Leica EM CPD300), mounted on metallic bases, and coated with a gold layer (Leica EM ACE200). The cells were visualized using a scanning electron microscope (JEOL JSM-6010 Plus/LA) operating at 10 keV.
de Oliveira H.C., Castelli R.F., Reis F.C., Samby K., Nosanchuk J.D., Alves L.R, & Rodrigues M.L. (2022). Screening of the Pandemic Response Box Reveals an Association between Antifungal Effects of MMV1593537 and the Cell Wall of Cryptococcus neoformans, Cryptococcus deuterogattii, and Candida auris. Microbiology Spectrum, 10(3), e00601-22.
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7

Scanning Electron Microscopy of Yeast Cells

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Yeast cells of PbWT, PbEV, PbDRK1 aRNA2, PbDRK1 aRNA3, and PbDRK1 aRNA4 were grown under standard conditions as mentioned above. Then, the cells were washed with PBS and then fixed with 2.5% glutaraldehyde in a 0.1 M sodium cacodylate buffer (pH 7.2) for 1 h at 25 °C. After washing with sodium cacodylate buffer containing 0.2 M sucrose and 2 mM MgCl2, the cells were seeded on poly-D-lysine (0.01%) and covered with coverslips and incubated for 30 min at 25 °C. A series of gradual ethanol dehydration (30, 50, and 70% for 5 min, 90% for 10 min, and 100% twice for 10 min) was performed followed by a critical-point drying (LEICA EM CPD300, Wetzlar, Germany), and mounting on metallic bases to be covered with a gold layer [40 (link)]. Images were obtained using a scanning electron microscope (JEOL JSM-6010 Plus/LA) operating at 5 keV.
Marcos C.M., de Oliveira H.C., Assato P.A., Castelli R.F., Fusco-Almeida A.M, & Mendes-Giannini M.J. (2021). Drk1, a Dimorphism Histidine Kinase, Contributes to Morphology, Virulence, and Stress Adaptation in Paracoccidioides brasiliensis. Journal of Fungi, 7(10), 852.
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8

Characterization of Hydrolyzed and Ultrasonicated DSNPs

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The particle size and surface charge of the synthesized DSNPs were analysed through Zetasizer, Nano series, HT Laser, ZEN3600 (Malvern Instrument, UK). Regarding the morphology of synthesized DSNPs was determined using analytical scanning electron microscope (SEM) JSM-6010PLUS/LA (JEOL, Japan). For determining the functional groups of DSP (unhydrolyzed), HCl hydrolysed DSP for 4 days, and 4 days HCl hydrolysed DSP treated for 9 min with ultrasound (90% amplitude) samples, FTIR spectrophotometer (PerkinElmer Spectrum Two, Serial no.103146, UK) in the range of 400 to 4000 cm−1 at a resolution of 8 cm−1 was used. The comparison of FTIR spectrum of these samples were done to provide insight of any changes in the structural and functional groups occurring in samples after hydrolysis and treatment using ultrasound.
Mostafa H., Airouyuwa J.O, & Maqsood S. (2022). A novel strategy for producing nano-particles from date seeds and enhancing their phenolic content and antioxidant properties using ultrasound-assisted extraction: A multivariate based optimization study. Ultrasonics Sonochemistry, 87, 106017.
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9

Microscopic Imaging and SEM Analysis

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Observations and body measurements were taken using an Olympus BX51 microscope with differential interference contrast (DIC). Photographs were taken with an attached Nikon D610 digital camera. Drawings were made with a camera lucida. A specimen was dehydrated in a series of progressive concentrations of hexamethyldisilazane (HMDS). Once air-dried, the specimen was mounted on aluminium stubs and gold-coated for observation in a JEOL JSM-6010Plus-LA scanning electron microscope at the Scanning Electron Microscopy Laboratory (LMEB), ECOSUR-Chetumal, Mexico.
Góngora-Garza G., Tovar-Hernández M.A, & de León-González J.A. (2024). Re-description of Parasphaerosyllisindica Monro, 1937 (Annelida, Syllidae), with the establishment of a new species from western Mexico. Biodiversity Data Journal, 12, e116082.
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10

Morphological Characterization of Thin Films

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The morphological structure and elemental composition of the samples were characterized using a scanning electron microscopy (SEM). Surface morphologies of sputter-deposited Ni and Cu–Ni thin film samples were imaged using JEOL JSM-6060LV and JEOL JSM-6010PLUS/LA SEM. Chemical analysis was carried out using a Thermo Scientific Ultradry EDS detector attached to the SEM.
Barbee B., Muchharla B., Adedeji A., Karoui A., Kumar Sadasivuni K., Sha M.S., Abdullah A.M., Slaughter G, & Kumar B. (2022). Cu and Ni Co-sputtered heteroatomic thin film for enhanced nonenzymatic glucose detection. Scientific Reports, 12, 7507.
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