Pdgfrβ creert2

All animal studies were performed with institutional ACUC approval within Johns Hopkins University, complying with all relevant ethical regulations (ACUC protocol # MO18M12). PDGFRβ-CreER^T2 and mT/mG mice were purchased from Jackson lab (stock No.029684 and No.007676). PDGFRβ-CreER^T2 and mT/mG mice were crossed to generate PDGFRβ-CreER^T2; mT/mG mice. Both genders of these animals were used. Animals were bred and housed under SPF conditions with no more than four adult mice per cage. 200μg of tamoxifen (TM, Sigma, T5648) dissolved in corn oil was injected intraperitoneally to PDGFRβ-CreER^T2; mT/mG mice at the age of 8 weeks for 4 days consecutively, using a previously validated TM administration schedule^{15 (link)}. Essentially no recombination was observed within the joint associated tissues with injection of TM free vehicle control.

All animals used in this study received humane care in compliance with the principles stated in the Guide for the Care and Use of Laboratory Animals, NIH Publication, 1996 edition, and the protocol was approved by the Institutional Animal Care Committee of Universityof Washington. The C57BL/6 and SCID/Beige mice were purchased from Charles River (Wilmington, MA). Lgr5-DTR-eGFP mice were originally generated by Genentech (Tian et al., 2011 (link)) and were kindly provided by Dr. Noah Shroyer at the Baylor College of Medicine. Krt7-CreER^T2 was kindly provided by Dr. Jianwen Que at Columbia University. Krt8-CreER^T2 was made by our laboratory as described (Zhang et al., 2012 ). C57BL/6-Gt(ROSA)26Sor^{tm1(HBEGF)Awai}/J, B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J, B6.129P2-Lgr5^{tm1(cre/ERT2)Cle}/J, Col1a2-CreER^T2, Pdgfrβ-CreER^T2, NG2-CreER^T2, B6.Cg-Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze}/J mice were from the Jackson Laboratory (Bar Harbor, ME). Male mice at the age of E15.5 to postnatal 27 weeks were used. Mice were genotyped by polymerase chain reaction using mouse genomic DNA from tail biopsy specimens. The sequences of genotyping primers and the expected band sizes for PCR are listed in Table S3. PCR products were separated electrophoretically on 1% agarose gels and visualized via ethidium bromide under UV light.