Mouse anti elav antibody

Primary antibodies and titers used in this study for immunostaining are as follows: Rabbit anti-Cleaved Caspase-3 (Asp175) antibody (Cell Signaling, 1:200), mouse anti-Elav antibody (Developmental Studies Hybridoma Bank, DSHB, 1:50), mouse anti-Repo (Developmental Studies Hybridoma Bank, DSHB, 1:50), rabbit anti-GFP (GeneTex, 1:200), mouse anti-NimC1 (a gift from Dr István Andó, 1:30)^{24 (link)}, rabbit anti-Eiger (a gift from Dr Chun-Hong Chen, National Health Research institute, NHRI, 1:200), rabbit anti-FasII (a gift from Dr Vivian Budnik, 1:5000)^{42 (link)} and rabbit anti-pFAK (Cell Signaling, 1:200). For western blotting, mouse anti-Aβ42 (6E10) (Covance, 1:5000), rabbit anti-phospho JNK (pTPpY) (Promega, 1:2000), rabbit anti-JNK (Santa Cruz, 1:5000), and rabbit anti-GFP (GeneTex, 1:5000) were used.

Immunofluorescence was performed on third instar larval eye discs and imaged as described [58 (link)], using the rabbit anti-beta Galactosidase antibody (Immunology Consultants Laboratory, Inc. 1:200) and mouse anti-Elav antibody (Developmental Studies Hybridoma Bank (DSHB) 1:50).