Recombinate

Manufactured by Baxter

Sourced in France

Recombinate is a recombinant human antihemophilic factor VIII product. It is used for the treatment and prevention of bleeding in individuals with hemophilia A.

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Lab products found in correlation

Apc labeled annexin 5, by BD (1 mentions)

2 protocols using recombinate

Evaluating FVIII and ADAMTS-13 Interactions

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Full length FVIII was either a kind gift from CSL-Behring (Helixate® NexGen, Marburg, Germany) or from Baxter (Recombinate®, Maurepas, France). Recombinant human A disintegrin and metalloprotease with thrombospondin type I repeats-13 (ADAMTS-13) was a kind gift from Baxter. Complement human proteins Factor B, Factor D, C3, C3b and C3-depleted serum were purchased from Complement Technology (Comptech, TX, USA) and Merck Millipore (Merck Chemicals Ltd., Nottingham, UK). Human serum was obtained from AB blood type healthy donors. Antibodies against CD1a, CD3, CD14, CD40, CD83, CD86, HLA-DR, CD206, low density lipoprotein receptor-related protein (LRP, CD91), CD209, CD68 and APC-labeled Annexin V were purchased from BD Pharmingen (San Jose, CA, USA). Antibody against CD20 was purchased from eBiosciences (San Diego, CA, USA).
The biotinylated monoclonal mouse anti-human FVIII antibody GMA-8015 and sheep polyclonal anti-human FVIII (SAF8C) were from Green Mountain Antibodies (Burlington, VT, USA) and Affinity Biological (Ancaster, Canada). The monoclonal anti-human FVIII antibody 77IP52H7 was a kind gift from LFB (Les Ulis, France). The biotinylated monoclonal mouse anti-human ADAMTS-13 antibody (20A5) and polyclonal goat anti-mouse C3b/iC3b (clone A209) were from Clinisciences (Nanterre, France) and Quidel (San Diego, USA), respectively.

Rayes J., Ing M., Delignat S., Peyron I., Gilardin L., Vogel C.W., Fritzinger D.C., Frémeaux-Bacchi V., Kaveri S.V., Roumenina L.T, & Lacroix-Desmazes S. (2018). Complement C3 is a novel modulator of the anti-factor VIII immune response. Haematologica, 103(2), 351-360.

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Quantifying anti-FVIII antibodies by ELISA

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ELISA plates were coated overnight at 4°C with recombinant FVIII (2 µg/ml, recombinate, Baxter, Maurepas, France). Plates were blocked with PBS 1% BSA. Serum in dilution was then incubated for 1 hr at 37°C. Bound IgG was revealed with a HRP-coupled monoclonal antimouse IgG antibody (Southern Biotech) and substrate. The mouse monoclonal anti-FVIII IgG mAb6 (a gift from Prof J. M. Saint-Remy, KUL, Belgium) was used as a standard.

Peyron I., Dimitrov J.D., Delignat S., Gangadharan B., Srivastava A., Kaveri S.V, & Lacroix-Desmazes S. (2018). Oxidation of factor VIII increases its immunogenicity in mice with severe hemophilia A. Cellular immunology, 325.

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