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Hcg elisa kit

Manufactured by Calbiotech
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The HCG ELISA Kit is a laboratory equipment used for the quantitative determination of human chorionic gonadotropin (hCG) in human serum or plasma samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure hCG levels.

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Lab products found in correlation

Dneasy kit, by Qiagen (1 mentions) Qubit dsdna br assay kit, by Thermo Fisher Scientific (1 mentions)

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ELISAs (2 citations)

3 protocols using hcg elisa kit

1

Quantifying hCG Levels in Cells

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Supernatants were collected from cells cultured over 48 hours. Levels of total hCG were quantified using an hCG ELISA Kit (Calbiotech) following the manufacturer’s protocol. The results were normalized to DNA content of cells from the same well, extracted using the DNeasy Kit (Qiagen).
Horii M., Moretto-Zita M., Nelson K.K., Li Y, & Parast M.M. (2015). MTA3 regulates differentiation of human cytotrophoblast stem cells. Placenta, 36(9), 974-980.
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2

Quantification of hCG in Cell Supernatants

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Cell culture supernatants were collected and stored at -80 °C until use. Levels of total hCG were quantified using the hCG ELISA Kit (HC251F, Calbiotech Inc.). The results were normalized to total DNA content, extracted by DNeasy (Qiagen), and quantified by Qubit dsDNA BR assay kit (ThermoFisher).
Horii M., Morey R., Bui T., Touma O., Nelson K.K., Cho H.Y., Rishik H., Laurent L.C, & Parast M.M. (2021). Modeling preeclampsia using human induced pluripotent stem cells. Scientific Reports, 11, 5877.
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3

Differentiation of hTS^PS Cells to STB

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hTSPS cells (1 × 105 per well) were seeded to differentiate into STB cells as described above. The supernatants were collected at day 6. As controls, hTSPS cells (1 × 105 per well) were seeded and cultured in TS medium. After day 2, the supernatants were collected and stored at −80°C. The level of secreted human chorionic gonadotropin (hCG) was measured using an hCG ELISA kit (Calbiotech).
Wei Y., Wang T., Ma L., Zhang Y., Zhao Y., Lye K., Xiao L., Chen C., Wang Z., Ma Y., Zhou X., Sun F., Li W., Dunk C., Li S., Nagy A., Yu Y., Pan G., Lye S.J, & Shan Y. (2021). Efficient derivation of human trophoblast stem cells from primed pluripotent stem cells. Science Advances, 7(33), eabf4416.
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