MAbs KJ1-26 (anti-DO11.10 clonotype) [30 (link)] and 23G2 (anti-CD45RB) [31 (link)], were prepared from the supernatants of hybridoma cell lines. Purchased Abs were: HRP-goat anti mouse IgG (Southern Biotech), HRP-goat anti mouse IgM (Southern Biotech), and Magnetic anti-goat mouse Ig microbeads, (Miltenyi Biotec). In addition, rabbit gamma globulin (RGG, Pel Freeze Biologicals, Rogers, AR) was purchased. Where indicated, RGG and BSA (Sigma) or whole chicken ovalbumin (OVA) (Sigma) were haptenated using 2,4,6-Trinitrobenzenesulfonic acid (J.T. Baker Chemical), as previously described. Chicken OVA peptide (OVA323-339) was synthesized and supplied by the Wadsworth Center Peptide Synthesis Core Facility. 5- (and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Inc., Eugene, OR) and SEB (Toxin Technology), and ELISPOT kits (Becton Dickenson) were purchased.
Magnetic anti goat mouse ig microbeads
Manufactured by Miltenyi Biotec
Magnetic anti-goat mouse Ig microbeads are designed for the labeling and isolation of cells expressing mouse immunoglobulin. The microbeads are coated with antibodies specific to mouse immunoglobulin, enabling the magnetic separation and enrichment of cells bound to the beads.
Automatically generated - may contain errors
Lab products found in correlation
2 4 6 trinitrobenzenesulfonic acid, by Merck Group (2 mentions)
Goat anti mouse igg hrp, by Southern Biotech (2 mentions)
5 and 6 carboxyfluorescein diacetate succinimidyl ester cfse, by Thermo Fisher Scientific (2 mentions)
Goat anti mouse igm hrp, by Southern Biotech (2 mentions)
Rgg and bsa, by Merck Group (2 mentions)
Whole chicken ovalbumin ova, by Merck Group (2 mentions)
Elispot kit, by BD (2 mentions)
2 protocols using magnetic anti goat mouse ig microbeads
1
Monoclonal Antibody Production and Reagents
2
Monoclonal Antibody Production and Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MAbs KJ1-26 (anti-DO11.10 clonotype) [30 (link)] and 23G2 (anti-CD45RB) [31 (link)], were prepared from the supernatants of hybridoma cell lines. Purchased Abs were: HRP-goat anti mouse IgG (Southern Biotech), HRP-goat anti mouse IgM (Southern Biotech), and Magnetic anti-goat mouse Ig microbeads, (Miltenyi Biotec). In addition, rabbit gamma globulin (RGG, Pel Freeze Biologicals, Rogers, AR) was purchased. Where indicated, RGG and BSA (Sigma) or whole chicken ovalbumin (OVA) (Sigma) were haptenated using 2,4,6-Trinitrobenzenesulfonic acid (J.T. Baker Chemical), as previously described. Chicken OVA peptide (OVA323-339) was synthesized and supplied by the Wadsworth Center Peptide Synthesis Core Facility. 5- (and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Inc., Eugene, OR) and SEB (Toxin Technology), and ELISPOT kits (Becton Dickenson) were purchased.
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