The INHBA/pMSCVneo and BMPRII-GFP/pMSCVneo constructs, alongside the pMSCVneo/GFP control construct, were transfected into GP2-293 packaging cells (Clontech) using a Lipofectamine 3000 (Thermo-Fisher) concurrent with a pVSVG viral envelope expression construct, followed by changing the medium with a fresh growth medium in 24 h. After an additional 24 h, the medium was changed again with a fresh growth medium and incubated for another 24 h. Subsequently, the culture medium containing retroviruses was collected and stored at −80 °C after the removal of floating cells by centrifugation.
Pmscvneo vector
The PMSCVneo vector is a plasmid-based expression vector used for the production of recombinant proteins in mammalian cell lines. It contains the necessary elements for gene expression, including a promoter and a selection marker for antibiotic resistance.
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7 protocols using pmscvneo vector
Retroviral Vector Construction and Packaging
The INHBA/pMSCVneo and BMPRII-GFP/pMSCVneo constructs, alongside the pMSCVneo/GFP control construct, were transfected into GP2-293 packaging cells (Clontech) using a Lipofectamine 3000 (Thermo-Fisher) concurrent with a pVSVG viral envelope expression construct, followed by changing the medium with a fresh growth medium in 24 h. After an additional 24 h, the medium was changed again with a fresh growth medium and incubated for another 24 h. Subsequently, the culture medium containing retroviruses was collected and stored at −80 °C after the removal of floating cells by centrifugation.
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