Alexa Fluor dyes 405, 488, and 555 were purchased from Thermo Fisher Scientific. POPC and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l -serine (POPS) were purchased from NOF Co., and bovine liver PI, TopFluor-TMR-PS, TopFluor-TMR-PC, and TopFluor-TMR-PE were from Avanti Polar Lipid. EGCg and tannic acid were purchased from Nacalai Tesque and Wako Chemicals, respectively.
Alexa fluor dyes 405
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa Fluor dyes 405 are a family of fluorescent dyes developed by Thermo Fisher Scientific. These dyes are designed for use in a variety of bioanalytical applications, including flow cytometry, microscopy, and protein labeling. The Alexa Fluor 405 dyes exhibit a peak excitation wavelength of 401 nm and a peak emission wavelength of 421 nm, making them suitable for detection using violet or ultraviolet light sources.
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2 protocols using alexa fluor dyes 405
1
Fluorescent Lipid Probes for Membrane Studies
2
Fluorescent Microscopy Sample Preparation
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Alexa Fluor dyes 405 and 488 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). POPC was purchased from NOF Co. (Osaka, Japan) and TopFluor-TMR-PS was obtained from Avanti Polar Lipid (Alabaster, AL, USA). CYTOP-A and AZP4903 were purchased from Asahi-Glass (Tokyo, Japan) and AZ Electronic Materials (Luxembourg), respectively. α-Hemolysin (HT101) was purchased from Toxin Technology (FL, USA). The D101S mutant of alkaline phosphatase (ALP) from Escherichia coli was expressed and purified according to previously described methods. 20
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