All chemicals were prepared and stored according to manufacturer recommendations unless otherwise noted. l -lactate oxidase (EC 1.1.3.2) was obtained from USBiological (Salem, MA; Cat: L3000-10) and diluted in distilled, deionized water to make a 1 IU/µl stock solution for storage at 4 °C. Sodium phosphate monobasic monohydrate, sodium phosphate dibasic anhydrous, 1,3 phenylenediamine dihydrochloride (mPD), sodium chloride, calcium chloride dihydrate, and H2O2 (30% in water) were obtained from Thermo Fisher Scientific (Waltham, MA). l -lactate sodium salt, bovine serum albumin (BSA), glutaraldehyde, dopamine hydrochloride (DA), l -ascorbic acid (AA), polyurethane (PU), tetrahydrofuran (THF), and N,N-Dimethylformamide (DMF) were obtained from Sigma-Aldrich Co. (St. Louis, MO). Human Aβ1-42 (Cat: AS-20276) and human scrambled Aβ1-42 (Cat: AS-25383) was obtained from AnaSpec (Fremont, CA) and stored at − 80 °C once reconstituted. CHC (Cat: 5029) and TBOA (Cat: 1223) was obtained from Tocris Bioscience (Minneapolis, MN) and stock solutions were stored at − 20 °C.
L lactate sodium salt
Manufactured by Merck Group
Sourced in Belgium
L-lactate sodium salt is a laboratory reagent used as a source of L-lactate ions. It is a white, crystalline solid that is highly soluble in water. L-lactate sodium salt is commonly used in various biochemical and cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Nisin a, by Merck Group (2 mentions)
Sodium phosphate monobasic monohydrate, by Thermo Fisher Scientific (1 mentions)
Dopamine hydrochloride da, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
Calcium chloride dihydrate, by Thermo Fisher Scientific (1 mentions)
Tetrahydrofuran thf, by Merck Group (1 mentions)
Human aβ1 42, by AnaSpec (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions)
Poly allylamine hydrochloride pah, by Merck Group (1 mentions)
Sodium chloride (nacl), by Carlo Erba (1 mentions)
L lactate oxidase, by Merck Group (1 mentions)
L histidine monohydrochloride monohydrate, by Merck Group (1 mentions)
Whatman, by Cytiva (1 mentions)
4 protocols using l lactate sodium salt
1
Lactate Oxidase-Based Biosensor Assay
2
Optimizing Bacterial Growth and Induction Conditions
3
Optimizing Bacterial Growth and Induction Conditions
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Bacterial strains and plasmids used in the present study are listed in Supplementary Table 9 . All plasmid constructions were performed in Escherichia coli DH10B for pUC18Ery derivatives and in Lc. lactis NZ3900 for pNZ8048 derivatives. L. plantarum was grown in MRS (De Man-Rogosa-Sharpe) broth at 28°C without shaking. Lc. lactis was grown in M17 broth supplemented with 0.5% glucose at 28°C at 120 rpm. When appropriate, chloramphenicol and erythromycin were added to the media at 10 μg ml-1 and NiCl2 at 1 mM concentration. For induction of Lar activity in L. plantarum, L-lactate sodium salt (Sigma-Aldrich, Belgium) was added at a concentration of 200 mM during the mid-log phase (OD600 = 0.6-0.7) and the cells were collected 4 h later. For the induction of genes under control of the nisA expression signals, Nisin A (Sigma-Aldrich, Belgium) was added during the early log phase (OD600 = 0.2-0.3) at a concentration of 1 mg l-1 and the cells were collected 4 h later.
4
Colorimetric Lactate Sensing Assay
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Peroxidase (type VI-A from horseradish, 1080 U/mg protein), Llactate oxidase (from Pediococcus sp., 50 U/mg protein), L-lactate sodium salt, 3,3′,5,5′-tetramethylbenzidine (TMB), Poly (allyl amine hydrochloride) (PAH), Poly(sodium 4-styrene sulfonate) (PSS) Lhistidine monohydrochloride mono-hydrate, mucin (type II from porcine stomach), urea and the chromatographic paper for assay support (Whatman CHR 1, in sheets of 20×20 cm)
were purchased from Sigma Aldrich (St. Louis, MO).
Sodium chloride, disodium hydrogen orthophosphate anhydrous, and sodium dihydrogen orthophosphate monohydrate were purchased from Carlo Erba Reagents S.r.l. (Milano, Italy) .
Phosphate buffered saline (0.1 mol L -1 PBS, pH 7.5) was employed as a buffer. Artificial oral fluid at pH 7.2 was prepared by dissolving 0.6 mg mL -1 Na2HPO4, 0.6 mg mL -1 anhydrous CaCl2, 0.4 mg mL -1 KCl, 0.4 mg mL -1 NaCl, 4.0 mg mL -1 mucin and 4.0 mg mL -1 urea in deionized water according to Tlili et al. (2010) . The colorimetric lactate enzymatic assay in the standard 96-well microtiter plate format (BioVision Incorporated L-Lactate colorimetric Assay Kit) was bought from BioVision Incorporated, Inc. U.S.A. and used according to the manufacturer's instructions. Salivettes cotton swabs for oral fluid sampling were purchased from Sarstedt, Germany.
were purchased from Sigma Aldrich (St. Louis, MO).
Sodium chloride, disodium hydrogen orthophosphate anhydrous, and sodium dihydrogen orthophosphate monohydrate were purchased from Carlo Erba Reagents S.r.l. (Milano, Italy) .
Phosphate buffered saline (0.1 mol L -1 PBS, pH 7.5) was employed as a buffer. Artificial oral fluid at pH 7.2 was prepared by dissolving 0.6 mg mL -1 Na2HPO4, 0.6 mg mL -1 anhydrous CaCl2, 0.4 mg mL -1 KCl, 0.4 mg mL -1 NaCl, 4.0 mg mL -1 mucin and 4.0 mg mL -1 urea in deionized water according to Tlili et al. (2010) . The colorimetric lactate enzymatic assay in the standard 96-well microtiter plate format (BioVision Incorporated L-Lactate colorimetric Assay Kit) was bought from BioVision Incorporated, Inc. U.S.A. and used according to the manufacturer's instructions. Salivettes cotton swabs for oral fluid sampling were purchased from Sarstedt, Germany.
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