The research described herein utilized the following equipment: a high resolution in-vivo X-ray microtomograph (SkyScan 1176, Bruker, Germany), a digital microscope (MOTICBA210, Motic, China), an ultrapure water system (ALH-600-U, China), a Thermo Scientific Q Exactive Quadrupole-Orbitrap Mass Spectrometer System (Thermo Fisher Scientific Inc, Grand Island, NY, USA), a rotary evaporator (EYELA n-1100, Shanghai Alang Instruments Co., China), and a microplate reader (Beijing Prolong New Technology Co., China).
Q exactive quadrupole orbitrap mass spectrometer system
Manufactured by Thermo Fisher Scientific
Sourced in China
The Q Exactive Quadrupole-Orbitrap Mass Spectrometer System is a high-resolution, accurate-mass (HRAM) instrument that combines a quadrupole mass filter with an Orbitrap mass analyzer. This system provides high-performance mass analysis capabilities for a variety of applications, including proteomics, metabolomics, and small molecule analysis.
Automatically generated - may contain errors
Lab products found in correlation
Ba210, by Motic (1 mentions)
Skyscan 1176, by Bruker (1 mentions)
Xcalibur quan browser, by Thermo Fisher Scientific (1 mentions)
Compound discoverer software, by Thermo Fisher Scientific (1 mentions)
Ultimate 3000 high performance liquid chromatograph, by Thermo Fisher Scientific (1 mentions)
Simca p software, by Sartorius (1 mentions)
2 protocols using q exactive quadrupole orbitrap mass spectrometer system
1
In-vivo X-ray Microtomography Analysis
2
Metabolomic Profiling of Competitive Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After mPFC samples were dissected from highly and low competitive mice, three-phase solvent system including MTBE, methanol and water was used to extract metabolites. Non-targeted metabolomics were carried out using Ultimate 3000 High Performance Liquid Chromatograph (Thermo Scientific). and the Q Exactive quadrupole-Orbitrap Mass Spectrometer System (Thermo Scientific). The medium polar metabolites were detected by positive and negative ion detection modes, and polar metabolites were isolated by hydrop interaction liquid chromatography. Compound Discoverer software (Thermo Scientific) was used to process full-scan and data-dependent metabolic profile for comprehensive component extraction. XCalibur Quan Browser (Thermo Scientific) was used to extract the AUCs of the total ion flow diagram. The quantitative information of metabolites was combined for the final statistical analysis. Multivariate data were analyzed by SIMCA-P software (Umetrics, Umea, Sweden). Univariate analyses including independent sample t-tests and P-value FDR adjustments, as well as metabolic enrichment analyses, were performed on the MetaboAnalyst.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!