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Rneasy micro clean up procedure

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The RNeasy Micro clean-up procedure is a laboratory technique used to purify RNA from small samples. It is designed to remove impurities and contaminants from RNA samples, ensuring high-quality RNA for subsequent analysis or applications.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions)

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RNeasy Micro (65 citations) RNeasy clean-up (8 citations) RNeasy procedure (3 citations) RNeasy Micro clean-up (2 citations)

2 protocols using rneasy micro clean up procedure

1

Extracting RNA and DNA from PBMCs

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Total RNA and DNA were extracted from PBMCs lysed in TRIzol (Thermo Fisher Scientific, Cat no. 15596026) by the acid guanidinium thiocyanate-phenol-chloroform extraction method. This was performed on 37 individuals from an independent Singapore cohort spanning healthy controls, mild, moderate, and severe COVID-19 (in this cohort, severe and critical samples were combined into a single category). The aqueous phase was processed using the Qiagen RNeasy Micro clean-up procedure to isolate RNA. The leftover interphase and organic phase were further processed using the Back extraction protocol (Thermo Fisher Scientific) to isolate genomic DNA. RNA and genomic DNA concentrations were estimated using PicoGreen (Cat no. P7589).
Lin Q.X., Rajagopalan D., Gamage A.M., Tan L.M., Venkatesh P.N., Chan W.O., Kumar D., Agrawal R., Chen Y., Fong S.W., Singh A., Sun L.J., Tan S.Y., Chai L.Y., Somani J., Lee B., Renia L., Ng L.F., Ramanathan K., Wang L.F., Young B., Lye D., Singhal A, & Prabhakar S. (2024). Longitudinal single cell atlas identifies complex temporal relationship between type I interferon response and COVID-19 severity. Nature Communications, 15, 567.
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2

Total RNA Extraction from D1 Mouse Cells

Cited 1 time
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Total RNA was extracted from D1 mouse cell line [15 (link)]. Total RNA was extracted with Trizol Reagent (Invitrogen) followed by RNeasy micro clean-up procedure (Qiagen) as per manufacturer's instructions. Total RNA integrity was assessed by Agilent 2100 Bioanalyzer (Agilent) and the RNA Integrity Number (RIN) was calculated; RNA sample had a RIN = 9.5.
Carrara M., Lum J., Cordero F., Beccuti M., Poidinger M., Donatelli S., Calogero R.A, & Zolezzi F. (2015). Alternative splicing detection workflow needs a careful combination of sample prep and bioinformatics analysis. BMC Bioinformatics, 16(Suppl 9), S2.
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