Lsm780 fcs inverted microscope

Manufactured by Zeiss

The LSM780-FCS is an inverted confocal laser scanning microscope from Zeiss. It is designed for high-resolution imaging and fluorescence correlation spectroscopy (FCS) applications. The microscope features a fully motorized system, a flexible laser configuration, and advanced optics for advanced imaging capabilities.

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Lab products found in correlation

Szx16 fluorescence stereomicroscope, by Olympus (1 mentions) Szx16, by Olympus (1 mentions) Ab6328, by Abcam (1 mentions)

Close spelling variants of this product

Inverted microscope (490 citations) LSM780/FCS (17 citations) LSM780 inverted (8 citations) LSM780 FCS microscope (5 citations) Inverted LSM780 microscope (3 citations)

2 protocols using lsm780 fcs inverted microscope

In Vivo Imaging of Transgenic Embryos

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The generation of transgenic animals and determination of developmental stages were performed following standardized protocols^{65 (link),66}. Animals at stage 43 were anaesthetized by bathing in 0.01% benzocaine^{65 (link)}. Live imaging was performed on an Olympus SZX16 fluorescence stereomicroscope. Time lapse movies were acquired using an Axio Zoom.V16 (Zeiss) stereomicroscope. Confocal images were acquired on a Zeiss LSM780-FCS inverted microscope.
For immunostaining, embryos were fixed in MEMFA at 4 °C overnight, washed in PBS, embedded in 2% low-melting temperature agarose and sectioned by vibratome into 200-µm-thick sections. Fibronectin was detected using mouse anti-Fibronectin antibody (IST-9, mouse monoclonal, ab6328, Abcam) at 5 µg/ml.

Prummel K.D., Hess C., Nieuwenhuize S., Parker H.J., Rogers K.W., Kozmikova I., Racioppi C., Brombacher E.C., Czarkwiani A., Knapp D., Burger S., Chiavacci E., Shah G., Burger A., Huisken J., Yun M.H., Christiaen L., Kozmik Z., Müller P., Bronner M., Krumlauf R, & Mosimann C. (2019). A conserved regulatory program initiates lateral plate mesoderm emergence across chordates. Nature Communications, 10, 3857.

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Axolotl Regeneration Imaging and Analysis

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Axolotl husbandry and experiments (non-free feeding stages) were performed at the CRTD/Center for Regenerative Therapies Dresden, Dresden, Germany. Transgenic animals were generated using Tol2 transposase following standard protocols (Khattak et al., 2014 (link)). For live imaging, the animals were anaesthetized by bathing in 0.01% benzocaine and imaged on an Olympus SZX16 fluorescence stereomicroscope. Embryos were staged as described previously (Armstrong and Malacinski, 1989 ).
For immunostaining, axolotl embryos were fixed in MEMFA at 4 °C overnight, washed in PBS, embedded in 2% low-melting temperature agarose, and sectioned by vibratome into 200 μm-thick sections. Fibronectin was detected using mouse anti-Fibronectin antibody (ab6328, Abcam) at 5 μg/mL. Confocal images were acquired on a Zeiss LSM780-FCS inverted microscope.

Kemmler C.L., Smolikova J., Moran H.R., Mannion B.J., Knapp D., Lim F., Czarkwiani A., Hermosilla Aguayo V., Rapp V., Fitch O.E., Bötschi S., Selleri L., Farley E., Braasch I., Yun M., Visel A., Osterwalder M., Mosimann C., Kozmik Z, & Burger A. (2023). Conserved enhancer logic controls the notochord expression of vertebrate Brachyury. bioRxiv.

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