For transwell assay, cells were seeded and plated into the upper chambers of the 24-transwell Boyden chamber wells (Costar, Bedford, MA, USA). Cells that migrated into the lower surface were fixed and stained with 0.1% crystal violet after being treated or untreated with CTX and PP242, alone or in combination for 24 hours. The number of migrated cells in five random microscopic fields per chamber was countered under a light microscope.
24 transwell boyden chamber wells
Manufactured by Corning
Sourced in United States
The 24-transwell Boyden chamber wells are a versatile laboratory equipment designed for cell migration and invasion assays. They consist of a 24-well plate format with a porous membrane insert that separates the upper and lower chambers. This setup allows for the assessment of cell migration or invasion capabilities in response to various stimuli or conditions.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using 24 transwell boyden chamber wells
1
Transwell Migration Assay with CTX and PP242
2
Cell Invasion Assay with Tyrphostin AG 1296
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the cell invasion assay, 1×106 cells were plated into the upper chambers of 24-transwell Boyden chamber wells (Costar, Bedford, MA, USA), and treated with tyrphostin AG 1296 at various concentrations for 8 hr. Following treatment, cells were fixed and stained with 0.1% crystal violet for imaging. Migrated cells were lysed with 10% acetic acid following imaging. The absorbance of lysate was measured at 595 nm by using a Nanodrop 2000 (Thermo Scientific, Inc., Wilmongton, DE, USA).
3
Quantitative Migration Assay with Ponatinib
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One-million cells were plated into the upper chambers of the 24-transwell Boyden chamber wells (Costar, Bedford, MA, USA). After treatment with ponatinib at different concentrations for 8 hours, the cells were fixed and stained with 0.1% crystal violet. After images were taken, the migrated cells were lysed with 10% acetic acid. The absorbance of lysate was measured at 595 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!