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Anti cd161 hp 3g10

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Anti-CD161 (HP-3G10) is a monoclonal antibody that recognizes the CD161 antigen, which is expressed on natural killer (NK) cells, certain T cell subsets, and other immune cell types. This antibody can be used for the detection and analysis of CD161-positive cells in various applications, such as flow cytometry and immunohistochemistry.

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Lab products found in correlation

Anti cd69 fn50, by Miltenyi Biotec (2 mentions) Anti vα24 c15, by Beckman Coulter (2 mentions) Anti vβ11 c21, by Beckman Coulter (2 mentions) Anti vα7.2 3c10, by BioLegend (2 mentions) Anti cd16 3g8, by BD (2 mentions) Facscanto 2 cytometer, by BD (2 mentions) Docosahexaenoic acid, by Merck Group (1 mentions) Nervonic acid, by Merck Group (1 mentions) Lignoceric acid, by Merck Group (1 mentions) Heptadecanoic acid, by Merck Group (1 mentions) Pe cy7, by BD (1 mentions) Anti cd94 clone hp 3b1, by Beckman Coulter (1 mentions) Anti nkg2d clone 149810, by R&D Systems (1 mentions) Antibody labeling kit, by Thermo Fisher Scientific (1 mentions) Anti siglecf, by BD (1 mentions) Anti amphiregulin, by R&D Systems (1 mentions) Anti il 13 ebio13a, by Thermo Fisher Scientific (1 mentions) Anti il 4 11b11, by BioLegend (1 mentions) Anti ccr3, by R&D Systems (1 mentions) Anti cd45ro uchl1, by BioLegend (1 mentions)

4 protocols using anti cd161 hp 3g10

1

Flow Cytometry Immunophenotyping of PBMCs

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Cell staining and the flow-cytometry-based phenotypic analyses of PBMCs and cells were performed according to standard flow cytometry methods. The following mAbs were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), phycoerythrin-Cyanine7 (PE/Cy7), phycoerythrin-Cyanine5, phycoerythrin-Cyanine5.5, allophycocyanin (APC), allophycocyanin-Vio7, View blue or View green: anti-CD25 (M-A251), anti-CD27 (M-T271), anti-CD31 (WM59), anti-CD45RA (HI100), anti-CD45RO (UCHL1), anti-CD197/CCR7 (3D12), anti-TCRαβ (T10B9), anti-TCRγδ(B1), anti-CD95 (DX2), anti-CD19 (HIB19), anti-CD21 (B-ly4), anti-IgM (G20-127), anti-IgD (IA6-2), anti-CD56 (B159) and anti-CD16 (3G8), all purchased from BD Biosciences and anti-CD3 (BW264/56), anti-CD4 (VIT4), anti-CD8 (BW135/80) and anti-CD69 (FN50) from Miltenyi Biotec. iNKT cells were detected by staining with anti-Vα24 (C15) and anti-Vβ11 (C21) (Beckman Coulter) and MAIT cells by staining with anti-Vα7.2 (3C10) and anti-CD161 (HP-3G10 (Biolegend). All data were collected on a FACS-Canto II cytometer (BD Biosciences) and analysed using FlowJo Version 9.3.2 software (TreeStar).
Martin E., Palmic N., Sanquer S., Lenoir C., Hauck F., Mongellaz C., Fabrega S., Nitschké P., Esposti M.D., Schwartzentruber J., Taylor N., Majewski J., Jabado N., Wynn R., Picard C., Fischer A., Arkwright P, & Latour S. (2014). CTP synthase 1 deficiency in humans reveals its central role in lymphocyte proliferation. Nature, 510(7504), 288-292.
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2

Fatty Acid and Antibody Analysis of Natural Killer Cells

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Bovine albumin fatty acid-free and the fatty acids (1) palmitic acid (C16:0), (2) stearic acid (C18:0), (3) oleic acid (C18:1), (4) arachidic acid (20:0), (5) arachidonic acid (20:4), (6) eicosapentaenoic acid (20:5), (7) dosapentanoic acid (C22:5). (8) docosahexaenoic acid (C22:6), (9) lignoceric acid (C24:0) nervonic acid (C24:1), and (10) heptadecanoic acid (C17:0), served as an internal standard, and were purchased from Sigma Aldrich.
The following antibodies were acquired from Beckman Coulter CD3-CD16/CD56+, anti- CD158a anti-KIR2DL1/S1 (clone EB6B and clone Z27.3.7), anti-CD94 (clone HP-3B1) and anti NKG2A (CD159a, clone Z199). From Becton Dickinson, were purchased following antibodies: anti-KIR3DL1 (clone DX9), anti NKp30 (clone P30-15), anti NKp46 (clone 9E2), anti CD160 (clone BY55). Anti-human anti-CD158b (anti-KIR2DL3, clone DX27), CD 158f (anti-KI2DL5, clone UP-R1), anti-NKP44 (clone P44-8), and anti CD161 (HP-3G10) were purchased from Biolegend. The anti NKG2D (Clone # 149810) was purchased from R&D Systems. The antibodies anti-CD158e1/e2 KIR3DL1 (clone REA168), anti-KIR2DS4/CD158f (clone JJC11.6) were purchased from Miltenyi Biotech. The anti-mouse Fab FITC, PE, PE-Cy7 and the isotype controls mouse IgG1, IgG2 labelled with FITC, PE or PC5 were purchased from Becton Dickinson.
De Sanctis J.B., Dumut D.C., Radzioch D, & Hajdúch M. (2020). Functionally Relevant Differences in Plasma Fatty Acid Composition and Expression of Cytotoxic and Inhibitory NK Cell Receptors between Healthy Young and Healthy Elder Adults. Nutrients, 12(12), 3641.
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3

Flow Cytometry Immunophenotyping of PBMCs

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Cell staining and the flow-cytometry-based phenotypic analyses of PBMCs and cells were performed according to standard flow cytometry methods. The following mAbs were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), phycoerythrin-Cyanine7 (PE/Cy7), phycoerythrin-Cyanine5, phycoerythrin-Cyanine5.5, allophycocyanin (APC), allophycocyanin-Vio7, View blue or View green: anti-CD25 (M-A251), anti-CD27 (M-T271), anti-CD31 (WM59), anti-CD45RA (HI100), anti-CD45RO (UCHL1), anti-CD197/CCR7 (3D12), anti-TCRαβ (T10B9), anti-TCRγδ(B1), anti-CD95 (DX2), anti-CD19 (HIB19), anti-CD21 (B-ly4), anti-IgM (G20-127), anti-IgD (IA6-2), anti-CD56 (B159) and anti-CD16 (3G8), all purchased from BD Biosciences and anti-CD3 (BW264/56), anti-CD4 (VIT4), anti-CD8 (BW135/80) and anti-CD69 (FN50) from Miltenyi Biotec. iNKT cells were detected by staining with anti-Vα24 (C15) and anti-Vβ11 (C21) (Beckman Coulter) and MAIT cells by staining with anti-Vα7.2 (3C10) and anti-CD161 (HP-3G10 (Biolegend). All data were collected on a FACS-Canto II cytometer (BD Biosciences) and analysed using FlowJo Version 9.3.2 software (TreeStar).
Martin E., Palmic N., Sanquer S., Lenoir C., Hauck F., Mongellaz C., Fabrega S., Nitschké P., Esposti M.D., Schwartzentruber J., Taylor N., Majewski J., Jabado N., Wynn R., Picard C., Fischer A., Arkwright P, & Latour S. (2014). CTP synthase 1 deficiency in humans reveals its central role in lymphocyte proliferation. Nature, 510(7504), 288-292.
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4

Identification and Analysis of Memory Th2 Cells

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Mouse memory Th2 cells were stained with anti-IL1rL1(ST2) (DIH9, BioLegend), and two subpopulations (ST2 low , ST2 high ) were purified by FACS. Memory Th2 cells were stimulated with immobilized anti-TCRb for 4 hours. Anti-Amphiregulin (206220, R&D Systems) was conjugated by ourselves using an Antibody Labeling Kit (Thermo Fisher Scientific) and used for intracellular staining of mouse samples. Anti-IL-5 (TRFK5, BioLegend), Anti-IL-4 (11B11, BioLegend) and Anti-IL-13 (eBio13A, eBioscience) were used for intracellular staining of mouse samples. Anti-Siglec-F (E50-2440, BD Biosciences), Anti-CCR3 (83101, R&D Systems), Anti-CD101 (Moushi101, eBioscience) and Anti-CD62L (MEL-14, BioLegend) were used for cell surface staining of mouse samples.
T cells from PBMC and dissociated cells from the ECRS polyps were stained with anti-CD4 (OKT4, BioLegend), anti-CD45RO (UCHL1, BioLegend), anti-CD161 (HP-3G10, BioLegend), anti-CRTH2 (BM16, BioLegend), anti-Lineage Cocktails (BioLegend) and anti-CD127 (A019D5, BioLegend). Anti-Amphiregulin (31221, R&D Systems) was conjugated by ourselves using an Antibody Labeling Kit (Thermo Fisher Scientific) and used for intracellular staining for human samples. Anti-IL-5 (TRFK5, BioLegend) and anti-IFNg (4S.B3, BioLegend) were used for intracellular staining of human samples.
Morimoto Y., Hirahara K., Kiuchi M., Wada T., Ichikawa T., Kanno T., Okano M., Kokubo K., Onodera A., Sakurai D., Okamoto Y, & Nakayama T. (2018). Amphiregulin-Producing Pathogenic Memory T Helper 2 Cells Instruct Eosinophils to Secrete Osteopontin and Facilitate Airway Fibrosis. Immunity, 49(1).
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