Uas myr gfp

Manufactured by BD

The UAS-myr::GFP is a laboratory equipment product that enables the expression of a green fluorescent protein (GFP) that is fused to a myristoylation signal (myr) and under the control of the upstream activation sequence (UAS) promoter. This product allows for the visualization and tracking of membrane-associated proteins in experimental settings.

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Lab products found in correlation

W1118, by BD (1 mentions) Npf gal4, by BD (1 mentions) Orco gal4, by BD (1 mentions) Mhc gal4, by BD (1 mentions)

2 protocols using uas myr gfp

Drosophila Genetics: Fly Stock Maintenance

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Unless otherwise indicated, we maintained all fly stocks at 25 °C and 60% relative humidity under a 12 h:12 h light:dark cycle on standard cornmeal-yeast-corn syrup medium with 1.5 g/L of the anti-fungal Tegosept. We acquired the following fly stocks from either the Bloomington Drosophila Stock Center (BDSC) or the Vienna Drosophila Resource Center (VDRC) for our experiments: Canton-S (BDSC #1), w¹¹¹⁸ (BDSC #5905), NPF-GAL4 (BDSC #25681, #25682), UAS-myr::GFP (BDSC #32197, #32198), UAS-NPF-IR (VDRC #108772), 386Y-GAL4 (BDSC #25410), NPFR^c01896 (BDSC #10747), NPFR^Def (BDSC #1982), Orco-GAL4 (BDSC #23292), Or22a-GAL4 (BDSC #9951), UAS-NPFR-IR (VDRC #9605). We generated the UAS-NPFR-RD and UAS-NPFR-RB stocks by cloning NPFR cDNAs from Drosophila heads, subcloning them into a pUAS-T attB-containing vector, and injecting the resulting plasmids into eggs of a fly stock containing an attP landing site (BDSC #9736) using standard techniques.

Lee S., Kim Y.J, & Jones W.D. (2017). Central peptidergic modulation of peripheral olfactory responses. BMC Biology, 15, 35.

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Drosophila RNAi Experiments with GRASP

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Only males were used in experiments at an age of 7–15 days post-eclosion.
For the RNAi experiments, crosses were maintained at 25C for 3 days, after which the progeny were shifted to 27C.
Flies were cultured in a humidified incubator at 25C on standard lab food containing per liter: 15 g yeast, 8.6 g soy flour, 63 g corn flour, 5g agar, 5g malt, 74 mL corn syrup.
Fly strains used in this study were from previous work (Rajan and Perrimon, 2012 (link)) and/or obtained from the Bloomington Drosophila stock center (BDSC): UAS-GRASP::GFP (BSDC# 8507, 8508), UAS-myr::GFP (BDSC#32197), UAS-Golgi-RFP (BDSC# 30908), Lpp-Gal4 (Brankatschk and Eaton, 2010 (link)), Mhc-Gal4 (Schuster et al., 1996 (link)), Df(3L)BSC552 (BDSC# 26502), Df(3L)BSC445 (BDSC# 24949). The following Transgenic RNAi Project (TRiP) lines were used: IP3R-RNAi (JF01957), IP3R-RNAi (HMC03228), AkhR-RNAi (JF03256), Luciferase-RNAi (JF01355), and GFP-RNAi (HMS00314). In addition, three independent GRASP RNAi lines, SH08449, SH08450 and SH08451, were generated by the TRiP. qPCR analyses showed that GRASP knockdown is >75% for all three lines. Finally, the following UAS lines were generated: UAS-GRASP::GFP, UAS-GRASP::GFP-CaMKII TtoD, UAS-GRASP::tagRFP-T, UAS-upd2::tagRFP-T.

Rajan A., Housden B.E., Wirtz-Peitz F., Holderbaum L, & Perrimon N. (2017). A mechanism coupling systemic energy sensing to adipokine secretion. Developmental cell, 43(1), 83-98.e6.

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