Model 6850 chromatograph

The essential oils were analyzed by gas chromatography–mass spectrometry using a model 6850 chromatograph (Agilent Technologies, Santa, Clara, CA, USA) coupled with a model 5975C mass spectrometer (Agilent Technologies) and an HP-5MS column (30 m × 0.25 mm, 0.25 µm; Agilent Technologies). For the sample, 1 µL of essential oil was injected in split mode. The separation conditions were as follows: an initial temperature of 70 °C for 2 min followed by two increases in the heating ramps; the first at 20 °C min⁻¹ to 230 °C, and the second at 8 °C min⁻¹ up to 280 °C and then maintained for 1 min, using helium as a carrier gas. The total analysis time was 17.25 min. The detected mass range was 35–750 m/z, the sample was ionized by electronic impact at 70 eV, and the ionization source temperature was 230 °C. The essential oil components were identified by comparison with the NIST version 8.0 library database (National Institute of Standards and Technology, Gaithersburg, MD, USA) and the retention index^{13 (link)}.

The ethanol extract-derivatized sample was analyzed by gas chromatography-mass spectrometry using a Model 6850 chromatograph (Agilent Technologies, Santa Clara, CA, USA) coupled with a Model 5975C mass spectrometer (Agilent Technologies) and HP-5MS column (30 m × 0.25 mm, 0.25 μm Agilent Technologies).
The derivatization mixture was evaporated, the derivatized sample was dissolved in HPLC hexane (High-Performance Liquid Chromatography solvent) (500 µl), and 1 µl of the sample was injected in split mode. An initial temperature of 100 °C was followed by a heating ramp up from 5 °C min⁻¹ to 300 °C. Helium was the carrier gas. The total analysis time was 40.0 min. The detected mass range was 35–600 m/z, the sample was ionized by electronic impact at 70 eV, and the ionization source temperature was 230 °C. The compounds were identified by comparison with the NIST version 8.0 library database (National Institute of Standards and Technology, Gaithersburg, MD, USA).

The ethanolic extract was analyzed by gas chromatography‒mass spectrometry using a Model 6850 chromatograph (Agilent Technologies, Santa Clara, CA, USA) coupled with a Model 5975C mass spectrometer (Agilent Technologies) and HP-5MS column (30 m × 0.25 mm, 0.25 μm Agilent Technologies).
The ethanolic extract sample (1 µl of the sample from a solution of 1 mg/ml) was injected in split mode. The initial temperature was 70 °C for 2 min followed by one heating ramp up from 15 °C min⁻¹ to 290 °C and then maintained for 6 min. Helium was the carrier gas. The total analysis time was 31.0 min. The detected mass range was 35–600 m/z, the sample was ionized by electronic impact at 70 eV, and the ionization source temperature was 230 °C. The compounds were identified by comparison with the NIST version 8.0 library database (National Institute of Standards and Technology, Gaithersburg, MD, USA).