Brain slices were incubated in 50% CUBIC-L (T3740, Tokyo Chemical Industry) in DDW for 1 hr and CUBIC-L for 12 hr at 37°C. After washing twice with PBS(−) for 30 min, the delipidated slices were cleared by incubations in 50% CUBIC-R + (M) (T3741, Tokyo Chemical Industry) in DDW and CUBIC-R + (M), each for 4 hr at 20–25°C (Tainaka et al., 2018 (link)).
Furuta T., Yamauchi K., Okamoto S., Takahashi M., Kakuta S., Ishida Y., Takenaka A., Yoshida A., Uchiyama Y., Koike M., Isa K., Isa T, & Hioki H. (2021). Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF. iScience, 25(1), 103601.
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