Lsab2 streptavidin horseradish peroxidase

Necropsy was performed on all subjects. Tissue samples of all major organs were collected for histopathologic and immunohistochemical (IHC) examination, immersion-fixed in 10% neutral buffered formalin, and processed for histopathology, as previously described (20 ). For IHC examination, specific anti-MARV VP40 protein rabbit primary antibody (Integrated BioTherapeutics) was used to detect MARV antigen. The secondary antibody used was biotinylated goat anti-rabbit IgG (Vector Laboratories), and LSAB2 streptavidin–horseradish peroxidase (Dako) was used to visualize antigen. Tissue sections were processed using the Dako Autostainer. Slides were developed with Dako diaminobenzidine chromogen and counterstained with hematoxylin. Non-immune rabbit IgG primary antibody was used as a negative control.

Necropsy was performed on all subjects. Tissue samples of all major organs were collected for histopathologic and immunohistochemical examination, immersion-fixed in 10% neutral buffered formalin, and processed for histopathology as previously described (20 (link)). For immuno-histochemistry, specific anti-NiV immunoreactivity was detected using an anti-NiV N protein rabbit primary antibody at a 1:5000 dilution (12 ). In brief, tissue sections were processed for immunohistochemistry using the Dako Autostainer. Secondary antibody used was biotinylated goat anti-rabbit IgG (Vector Laboratories) at 1:200 followed by Dako LSAB2 streptavidin–horseradish peroxidase. Slides were developed with Dako DAB chromagen and counterstained with hematoxylin. Nonimmune rabbit IgG was used as a negative control.