To evaluate the bioactivity of insulin after the production process, the hypoglycemic effect of the released insulin from IPC-NEs or IPC was evaluated in normal rats. Briefly, insulin released from IPC-NEs or IPC at 37°C was collected and the concentration was determined by HPLC. Male Sprague-Dawley (SD) rats (200–250 g) (fasted for 16 hrs with free access to water) were injected subcutaneously with the released insulin or free insulin at a dose of 1 IU/kg (n=6). The blood glucose level was determined using the Roche ACCU-CHEK Go (Basel, Switzerland) glucose-meter at the predesigned time point.
Accu chek go
Manufactured by Roche
Sourced in Germany, Switzerland
The Accu-Chek Go is a compact and portable blood glucose monitoring system designed for easy and convenient use. It is a device that allows individuals to measure their blood glucose levels quickly and accurately.
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37 protocols using accu chek go
1
Evaluating Insulin Bioactivity in Rats
2
Glucose and β-Hydroxybutyrate Measurement
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Blood glucose was measured using an Accu-Chek Go glucometer (Roche Diagnostics). β-Hydroxybutyrate content was measured using Optium β-ketone test strips with Optium Xceed sensors (Abbott Diabetes Care).
3
Wistar Rat Diabetic Model Protocol
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A total of 50 male Wistar rats (200–240 g) were obtained from the Faculty of Pharmacy’s animal facility at King Abdulaziz University. The Research Ethics Committee, Faculty of Pharmacy, approved the experimental protocol (PH-1443-29). A temperature of 22 ± 2 °C was used to house the animals in 12 h day–night cycles. IP injection of streptozotocin (50 mg/kg) was used for diabetes induction, as described previously [25 (link)]. Fasting blood glucose level was checked once weekly during the course of the experiment using Accu-Chek Go (Roche, Mannheim, Germany). A range of blood glucose levels from 250 to 350 mg/dL was considered as moderate experimentally induced diabetes, and was used as the inclusion criterion for rats in the present investigation.
4
Intraperitoneal Glucose Tolerance Test
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The intraperitoneal glucose tolerance test was performed after a 16-hour fast following the 2-day wash-out period via EX intervention. Glucose water solution at a concentration of 250 mg× mL−1 was injected at a dose of 2.5 g×kg−1 body weight. The blood glucose level in tail vein blood was determined using a glucometer (Accu-Chek Go, Roche Diagnostic, Mannheim, Germany). The blood glucose levels at 0, 30, 60, and 120 minutes were measured after glucose water injection. Tail snipping method was used to obtain blood.
5
Glucose Tolerance Test in Mice
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Mice were injected with 1.5 mg glucose/g body weight after 6 h fast. Blood glucose was determined from tail blood using the ACCU-CHEK Go glucometer (Roche, Munich, Germany).
6
Glucose and Insulin Tolerance Testing
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GTT and ITT were performed in fed state. Mice were injected with 1 g of glucose per kg body weight for GTT, and 0.75 U of insulin per kg body weight for ITT, intraperitoneally. Blood was withdrawn from the tail vein at indicated times for glucose and/or insulin assessment. Blood glucose was measured using an “Accu-Chek Go” glucometer (Roche Diagnostics).
7
Glucose Tolerance Test in Rats
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Animal procedures were undertaken according to the guidelines of the Polish Ethical Committee on Animal Research, and approved by the Local Ethics Committee for Animal Experiments in Gdańsk, Poland.
Thirty-two male Wistar Han rats (∼10 weeks; Tri-City Academic Laboratory Animal Centre-Research and Services Centre, Medical University of Gdansk) were fasted for 16 h before experimentation. Animals underwent gavage with CPL500036 (0.3 mg/kg, 1 mg/kg or 3 mg/kg) or vehicle 2 h before receiving a glucose bolus (2 g/kg, i.p.; MilliporeSigma). Olanzapine (10 mg/kg) was used as an active comparator and delivered (s.c.) 30 min before the glucose bolus. Blood samples from the tail vein were collected from each rat right before drug administration, just before glucose loading as well as 15 min, 30 min, 60 min, 120 min, and 180 min after glucose administration. The glucose concentration was measured using a glucometer (Accu-ChekGo, Roche Diagnostics, Mannheim, Germany).
Thirty-two male Wistar Han rats (∼10 weeks; Tri-City Academic Laboratory Animal Centre-Research and Services Centre, Medical University of Gdansk) were fasted for 16 h before experimentation. Animals underwent gavage with CPL500036 (0.3 mg/kg, 1 mg/kg or 3 mg/kg) or vehicle 2 h before receiving a glucose bolus (2 g/kg, i.p.; MilliporeSigma). Olanzapine (10 mg/kg) was used as an active comparator and delivered (s.c.) 30 min before the glucose bolus. Blood samples from the tail vein were collected from each rat right before drug administration, just before glucose loading as well as 15 min, 30 min, 60 min, 120 min, and 180 min after glucose administration. The glucose concentration was measured using a glucometer (Accu-ChekGo, Roche Diagnostics, Mannheim, Germany).
8
Fish Blood Glucose Measurement
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Fish was sacrificed by spinal transaction and 1.5 μl of blood collected for measured blood glucose concentration by the method developed by Tang et al. (2014) (link) using a blood glucose monitor (ACCU-CHEK GO, Roche, Mannheim, Germany).
9
Measuring Body Weight, Food Intake, and Blood Glucose
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Body weight was measured every week on Monday morning and at the end of the experiment. Food intake was measured and corrected for spillage by weighing the jars containing food every week between 9:00 AM and 10:00 AM. Data are expressed as calories (cal)/day·BW (g). To measure blood glucose levels, blood was sampled 1 day before sacrifice (9:00 AM) by 'tail nick' and analyzed using a portable glucose monitor (ACCU-CHEK GO; Roche, Germany).
10
Streptozotocin-Induced Diabetic Rat Model
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The rats were given a single intraperitoneal dose of STZ 50 mg/kg body weight (Alfa Aesar, A Johnson Matthey Company, MA/USA, CAS: 18883-66-4) to produce DM [15 ]. Random blood sugar (RBS) was measured from a blood drop drawn from the tail vein using glucometer (Accu - Chek Go, Roche Diagnostic, Germany) after 4 days of STZ injection. The rats with RBS >300 mg/dl were enrolled in the study. Insulin neutral protamine Hagedorn (5 IU/kg body weight; Wosulin, Wockhardt Limited, India) was given subcutaneously, once a day to maintain the RBS between 250 and 350 mg/dl.
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