A 175 base pair (bp) fragment of the human IL-6 gene spanning the promoter IL-6 G-174C region was amplified with gene specific primers (Roche Diagnostics, Alameda, California, USA). The resulting PCR fragments were analyzed with hybridization probes labeled with LightCycler Red 640. The genotypes were identified by running a melting curve with specific melting points (Tm) (Tib Molbiol GmbH, Berlin, Germany).
Genetic polymorphism in a SNP located near the IL-28B gene (rs12979860) was determined by enzymatic digestion of the PCR product. DNA fragments were amplified by using specific primers. Primer sequences were 5′-GCCTGTCGTGTACTGAACCA-3′, and 5′-GCTCAGGGGTCAAATCACAGAAG-3′, a PCR product of 143 bp was digested with HhaI enzyme and the resulting fragments of 27, 38, 65 and 78 bp were separated on 20% acrylamide gel followed by silver staining (Fig. 1 ).
Genetic polymorphism in a SNP located near the IL-28B gene (rs12979860) was determined by enzymatic digestion of the PCR product. DNA fragments were amplified by using specific primers. Primer sequences were 5′-GCCTGTCGTGTACTGAACCA-3′, and 5′-GCTCAGGGGTCAAATCACAGAAG-3′, a PCR product of 143 bp was digested with HhaI enzyme and the resulting fragments of 27, 38, 65 and 78 bp were separated on 20% acrylamide gel followed by silver staining (