Anti phospho lats1

Cellular or tissue samples were lysed with RIPA lysis buffer freshly adding cocktail protease inhibitor (Thermo Scientific). Equal amounts of cellular proteins were subjected to electrophoresis in SDS-PAGE, and transferred to nitrocellulose membranes (Millipore). The membranes were blocked and then incubated at 4 °C overnight with indicated first antibodies, followed by incubation with the appropriate HRP-conjugated secondary antibodies (Thermo Scientific). The protein bands were detected and analyzed with an ECL. The following antibodies were used: anti-Aurora A (Upstate, #07-648), anti-phospho-Aurora A (Thr288) (Cell Signaling Technology, #3079), anti-YAP (Cell Signaling Technology, #4912), anti-phospho-YAP(ser127) (Cell Signaling Technology, #4911), anti-phospho-YAP(Ser397) (Cell Signaling Technology, #13619), anti-Lats1 (Cell Signaling Technology, #3477 P), anti-phospho-Lats1 (Thr1079) (Cell Signaling Technology, #9654P), anti-Lats2 (Rui Ying biological, China, #RLP1047), anti-SAV1 (Cell Signaling Technology, #3507P), anti-MST1 (Cell Signaling Technology, #3682P), anti-GAPDH (Kangcheng, China,#KC-5G4), anti-Lamin B1 (Epitomics, #6581-1), anti-CTGF (Life Science Products & Services, #AB60212a), anti-Myc Tag (MERCK, 05-724).

Anti-phospho-acetyl-CoA carboxylase (ACC), anti-phospho-AMPKα, anti-phospho-LKB1, anti-Phospho-LATS1, anti-phospho-YAP, anti-YAP, anti-Poly (ADP-ribose) polymerases (PARP), anti-caspase-3, anti-B-cell lymphoma-extra-large (Bcl-xL), and anti-β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA). HRP-conjugated anti-rabbit IgG and HRP-conjugated anti-mouse IgG were bought from Enzo Life Sciences (Farmingdale, NY, USA). Polyethylene glycol (PEG) 400 is manufactured by Yakury Pure Chemical Co., Ltd. (Kyoto, Japan). SC was produced by water extraction of the medicinal herb, Spatholobi Caulis (Daewon pharmacy, Daewon, Korea) as previously described [17 (link),19 (link)]; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), rhodamine 123 (Rh123), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), arachidonic acid (AA), ferric nitrilotriacetic acid (iron), Harris hematoxylin and eosin (H&E), and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).