Benchtop 1kb dna ladder
The BenchTop 1kb DNA Ladder is a molecular weight marker used to determine the size of DNA fragments in gel electrophoresis. It contains a set of DNA fragments ranging from 250 to 10,000 base pairs, allowing for accurate sizing of samples within this range.
Lab products found in correlation
3 protocols using benchtop 1kb dna ladder
Amplification and Extraction of Plasmids
Dendrimer-DNA Complexation Assay
at different N/P ratios (5, 15, and 30) were prepared following complexation
instructions of the transfection protocol. 250 ng of pGFP or model
DNA (herring sperm for proof-of-concept assays), both from Promega,
was used to form complexes with a proper dilution of the dendrimer
in deionized water. Complexes were then diluted to 20 μL final
volume, and 4 μL of loading dye (blue/orange loading dye, 6×
from Promega) was added to dendriplexes. 24 μL of each sample
was loaded on 0.8% (w/v) agarose gel. DNA ladder (BenchTop 1 kb DNA
Ladder, from Promega) was loaded in the first lane, while free DNA
was loaded in the last one. Electrophoresis running was performed
in Tris-acetate-EDTA buffer (1× TAE) at 150 V for 30 min. The
gel was subsequently placed into a plastic tray and incubated with
staining solution (Diamond Nucleic Acid Dye Promega diluted in 1×
TAE buffer) following the manufacturer’s instructions, for
20 min under gentle shaking, protected from light. The gel was analyzed
under UV Transilluminator BIO-RAD ChemiDoc XRS+ using a proper filter.
RFLP-based Genotypic Classification of PsHV
The resulting DNA fragments were separated by electrophoresis on a 0.5% agarose gel in 1× Tris–acetate–EDTA buffer (Sigma–Aldrich, St. Louis, MO, USA) containing 5 μL of ethidium bromide (10 mg/mL) (Life Technologies, Grand Island, NE, USA) at room temperature under constant voltage of 20 V for about 15 h. The BenchTop 1 kb DNA Ladder (Promega, Madison, WI, USA) was used as a DNA size marker. The gels were observed at 254 nm using a MacroVue UV transilluminator (GE Healthcare, Cleveland, OH, USA) and photographed.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!