MSeA was purchased from Aldrich (Saint-Louis, MO, USA); GSH, GSSG, NADPH, DTT, DMDSe, and TCEP from Sigma (Saint-Louis, MO, USA). DMDSe was prepared as a 20 mM stock solution in 100% ethanol stored at −20 °C. Glutathione reductase from baker’s yeast (205 units/mg, 2.2 mg/mL) was from Sigma (Saint-Louis, MO, USA). Yeast thioredoxin (9.7 units/mg, 88.5 µM) was from Fisher Scientific (Waltham, MA, USA). Yeast thioredoxin reductase (25.6 units/mg) was purchased from Abnova (Taipei, Taiwan).
Dmdse
Manufactured by Merck Group
Sourced in United States
The DMDSe is a laboratory equipment product manufactured by Merck Group. It serves as a core piece of equipment for various scientific applications, providing essential functionality for research and analysis purposes. The DMDSe's primary function is to perform precise measurements and data collection, though its specific applications may vary depending on the user's requirements. No further details or interpretations are provided.
Automatically generated - may contain errors
Lab products found in correlation
Dithiothreitol (dtt), by Merck Group (2 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Glutathione reductase from baker s yeast, by Merck Group (1 mentions)
Nadph, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Bio-Rad (1 mentions)
Mouse monoclonal anti myc antibodies 9e10, by Merck Group (1 mentions)
2 protocols using dmdse
1
Thioredoxin System Assay Protocol
2
Synthesis and Analysis of Selenium Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DMDSe (Sigma, Saint-Louis, MO, USA)) was prepared as a 20 mM stock solution in 100% ethanol and stored at −20 °C. MeSeSG was prepared according to [21 (link)]. L-SeMet and DTT were from Sigma. IAA and SDS were from BioRad (Hercules, CA, USA). Rabbit polyclonal anti-CPY antibodies were from Abcam (Cambridge, UK). Mouse monoclonal anti-myc antibodies (9E10) were from Sigma.
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