Migration assays were assessed by transwell cell culture chambers 5 mm pores (Corning Incorporated, Corning, NY, USA). In brief, 1 × 105 pre‐treated macrophages were suspended in 100 ml DMEM containing 0.5% FBS and added to the upper compartment of the insert. The lower chamber was filled with 100 ml DMEM containing 10% FBS. After incubation for 6 hrs at 37°C in a 5% CO2 incubator, cells were scraped from the upper surface, duplicate membranes fixed and migrated cells stained with crystal violet. Cells were counted in five random fields.
Transwell cell culture chambers 5 mm pores
Manufactured by Corning
Sourced in United States
Transwell cell culture chambers with 5 mm pores are a laboratory product designed to facilitate the study of cell migration and cell-cell interactions. These chambers consist of an upper and lower compartment separated by a porous membrane, allowing for the controlled exchange of media and signaling molecules between the two compartments.
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Lab products found in correlation
2 protocols using transwell cell culture chambers 5 mm pores
1
Transwell Migration Assay of Macrophages
2
Migration Assay Using Transwell Chambers
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Migration assays were assessed by transwell cell culture chambers 5 mm pores (Corning Incorporated, Corning, NY, USA). In brief, 3×105 H226 cells and H460 cells were suspended in 100 ml RPMI 1640 without FBS and then added to the upper compartment of the insert. The lower chamber was filled with 100 mL RPMI 1640 containing 10% FBS. After incubation for 6 h at 37 °C in a 5% CO2 incubator, cells were scraped from the upper surface, duplicate membranes fixed, and migrated cells stained with crystal violet. Cells were counted in 5 random fields and the mean cell number were calculated.
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