T4 tri phosphor 6500 k daylight fluorescent tubes

661 W cells were exposed to 15,000 lux light (2.2 mW/cm²; irradiance measured with PM100D optical power meter, THORLABS, NJ, USA) from two white fluorescent lamps (2 × 10 W T4 tri-phosphor 6500 K daylight fluorescent tubes; Crompton, NSW, Australia), for 5 hours with 5% CO₂ at 37 °C. For dim control cells, one plate and one chamber slide in the incubator were completely wrapped in aluminium foil to avoid light exposure. For air/gas exchange, six small incisions were cut on the aluminium foil. Following incubation, cells in both the dim and photo-oxidative damage chamber slides were washed in PBS before being fixed in 4% PFA for 2 hours at 4 °C and then were maintained in PBS at 4 °C until further use. Cells in each of the 24 wells were washed with PBS and were then triturated either in TRIzol (Thermo Fisher Scientific, MA, USA) and stored at −80 °C until further use (n = 6 per experimental group), or in placed into CellLytic M buffer (Sigma-Aldrich, MO, USA) containing a Protease Inhibitor Cocktail (Sigma-Aldrich, MO, USA) and then stored at −80 °C until further use (N = 6 per experimental group).

661W cells were seeded in 96 well plates (Nunc, Thermo Fisher Scientific, MA, United States) at 2 × 10⁴ cells/well 24 h prior to photo-oxidative damage experiments. Cells were exposed for 4 h to 15,000 lux light (2.2 mW/cm²; irradiance measured with PM100D optical power meter, Thorlabs, NJ, United States) from two white fluorescent lamps (2 × 10W T4 tri-phosphor 6500K daylight fluorescent tubes; Crompton, NSW, Australia) as published previously (Lu et al., 2017 (link); Fernando et al., 2018 ). Control cells were completely wrapped in aluminum foil with six small incisions to allow air/gas exchange.