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Polyethylene terephthalate slides

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Polyethylene terephthalate slides are a type of laboratory equipment used for various microscopy techniques. They are made of a clear, durable plastic material that is suitable for specimen mounting and observation. These slides provide a stable and uniform surface for samples, enabling clear visualization and analysis under a microscope.

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Lab products found in correlation

Md lmd laser microdissecting microscope, by Leica (2 mentions) Cells to ct kit, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Polyethylene terephthalate frame slides Non-fluorescent polyethylene terephthalate (PET) microscope steel frame slide

2 protocols using polyethylene terephthalate slides

1

Hypoxic Tumor Tissue Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
In vivo tumor samples from a previous study46 (link) injected with Hypoxyprobe (Hypoxyprobe Inc., Burlington, MA) were used for the present study. Frozen sections (10 μm) were affixed onto polyethylene terephthalate slides (Leica), fixed in cold acetone (10 minutes), washed in PBS 3 times, stained for Hypoxyprobe using FITC-conjugated antibody for 1 hour on top of ice, washed, and air-dried; dissection immediately followed. Microdissection was performed using an MD LMD laser microdissecting microscope (Leica). RNA was isolated from some samples using the Cells to Ct Kit (Applied Biosystems), according to manufacturer instructions. Real-time PCR was carried out to quantify gene expression.
Rupaimoole R., Wu S.Y., Pradeep S., Ivan C., Pecot C.V., Gharpure K.M., Nagaraja A.S., Armaiz-Pena G.N., McGuire M., Zand B., Dalton H.J., Filant J., Miller J.B., Lu C., Sadaoui N.C., Mangala L.S., Taylor M., van den Beucken T., Koch E., Rodriguez-Aguayo C., Huang L., Bar-Eli M., Wouters B.G., Radovich M., Ivan M., Calin G.A., Zhang W., Lopez-Berestein G, & Sood A.K. (2014). Hypoxia Mediated Downregulation of miRNA Biogenesis Promotes Tumor Progression. Nature communications, 5, 5202.
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2

Hypoxic Tumor Tissue Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
In vivo tumor samples from a previous study46 (link) injected with Hypoxyprobe (Hypoxyprobe Inc., Burlington, MA) were used for the present study. Frozen sections (10 μm) were affixed onto polyethylene terephthalate slides (Leica), fixed in cold acetone (10 minutes), washed in PBS 3 times, stained for Hypoxyprobe using FITC-conjugated antibody for 1 hour on top of ice, washed, and air-dried; dissection immediately followed. Microdissection was performed using an MD LMD laser microdissecting microscope (Leica). RNA was isolated from some samples using the Cells to Ct Kit (Applied Biosystems), according to manufacturer instructions. Real-time PCR was carried out to quantify gene expression.
Rupaimoole R., Wu S.Y., Pradeep S., Ivan C., Pecot C.V., Gharpure K.M., Nagaraja A.S., Armaiz-Pena G.N., McGuire M., Zand B., Dalton H.J., Filant J., Miller J.B., Lu C., Sadaoui N.C., Mangala L.S., Taylor M., van den Beucken T., Koch E., Rodriguez-Aguayo C., Huang L., Bar-Eli M., Wouters B.G., Radovich M., Ivan M., Calin G.A., Zhang W., Lopez-Berestein G, & Sood A.K. (2014). Hypoxia Mediated Downregulation of miRNA Biogenesis Promotes Tumor Progression. Nature communications, 5, 5202.
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