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Sab4200135

Manufactured by Merck Group
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The SAB4200135 is a laboratory equipment product from Merck Group. It is a precision instrument designed for conducting scientific experiments and analyses in a laboratory setting. The core function of this product is to provide accurate and reliable measurements or data collection as required by the user's research or testing needs.

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Lab products found in correlation

Pgipz vector, by Thermo Fisher Scientific (1 mentions) Arrest in transfection reagent, by Thermo Fisher Scientific (1 mentions) Wortmannin, by Thermo Fisher Scientific (1 mentions) Ab105459, by Abcam (1 mentions) Ab117819, by Abcam (1 mentions) Rapamycin, by Merck Group (1 mentions) Mouse anti myc, by Merck Group (1 mentions) Lysotracker deep red, by Thermo Fisher Scientific (1 mentions) Mouse anti lamp1, by BD (1 mentions) Bafilomycin, by Merck Group (1 mentions) Mouse anti gfp, by Roche (1 mentions) Rabbit anti ulk1, by Cell Signaling Technology (1 mentions) Mouse anti actin, by Proteintech (1 mentions) Rabbit anti fip200, by Proteintech (1 mentions) Rabbit anti fip200, by Merck Group (1 mentions) Rabbit anti vapa, by Proteintech (1 mentions) Rabbit anti myc, by Cell Signaling Technology (1 mentions) Rabbit anti atg13, by Cell Signaling Technology (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Rabbit anti lc3, by Cell Signaling Technology (1 mentions)

3 protocols using sab4200135

1

Generation of RB1CC1 Knockdown Cell Lines

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Stable knockdown cell lines were generated by transfecting PC3 cells with pGIPZ vectors (Open Biosystems) for RB1CC1 (Clone ID #1 V3LHS-307113; Clone ID #2 V3LHS-229368), non-silencing (NS), and GAPDH using Arrest-In Transfection Reagent (Open Biosystems), followed by puromycin selection. These cell lines were named PC3-NS, PC3-GAPDH, PC3-RB1CC#1, and PC3-RB1CC1#2. Expression of RB1CC1 and GAPDH in knockdown cells was analyzed by qPCR. RB1CC1 knockdown was also confirmed by western blot using a polyclonal antibody raised against the c-terminal region (Sigma; SAB4200135). HEK293T and HEK293T transfected with p3XFLAG-CMV10-hFIP200 expression vector (Addgene) were used as controls.
Suraneni M.V., Moore J.R., Zhang D., Badeaux M., Macaluso M.D., DiGiovanni J., Kusewitt D, & Tang D.G. (2014). Tumor-suppressive functions of 15-Lipoxygenase-2 and RB1CC1 in prostate cancer. Cell Cycle, 13(11), 1798-1810.
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2

Autophagy Protein Immunodetection Assays

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The following antibodies were used for immunostaining or immunoblotting assays: mouse anti-LC3 (MBL, M152-3), rabbit anti-LC3 (2775S; Cell Signaling Technology), rabbit anti-p62 (MBL, PM045), rabbit anti-FIP200 (SAB4200135; Sigma-Aldrich), rabbit anti-FIP200 (17250-1-AP; Proteintech), rabbit anti-ULK1 (8054; Cell Signaling Technology), rabbit anti-Atg13 (13468S; Cell Signaling Technology), rabbit anti-ATL2 (16688-1-AP; Proteintech), rabbit anti-ATL3 (ab117819; Abcam), mouse anti-WIPI2 (ab105459; Abcam), mouse anti-VAPB (66191-1-IG; Proteintech), rabbit anti-VAPA (15275-1-AP; Proteintech), mouse anti-LAMP1 (553792; BD Biosciences), rabbit anti-PTPIP51 (20641-1-AP; Proteintech), rabbit anti-ATG101 (13492; Cell Signaling Technology), rabbit anti-Myc (2278S; Cell Signaling Technology), mouse anti-Myc (M5546; Sigma-Aldrich), mouse anti-GFP (11814460001; Roche), mouse anti-actin (60008-1-IG; Proteintech), and rabbit anti-HA (H6908; Sigma-Aldrich).
The following reagents were used in this study: wortmannin (PHZ1301; Life Technologies), bafilomycin (B1793; Sigma-Aldrich), rapamycin (R0395; Sigma-Aldrich), Lipofectamine 2000 (12566014; Life Technologies), Lipofectamine RNAi MAX (13778150; Life Technologies), and LysoTracker Deep Red (L12492; Thermo Fisher Scientific).
Liu N., Zhao H., Zhao Y.G., Hu J, & Zhang H. (2021). Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. The Journal of Cell Biology, 220(7), e202012091.
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3

Antibody Panel for Autophagy Assays

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Primary antibodies used were as follows: rabbit anti‐human Tuj1 (Covance, IF: 1:500), mouse anti‐FLAG (M2, Sigma, WB: 1:2,000, IF: 1:1,000), mouse anti‐Myc (9B11, Cell Signaling, WB: 1:2,000, IF: 1:2,000), mouse anti‐tubulin (DM1A, Sigma, WB: 1:10,000), rabbit anti‐Myc (ab9106, Abcam, WB: 1:2,000, IF: 1:1,000), rabbit anti‐GAPDH (14C10, Cell Signaling, WB: 1:2,000), rabbit anti‐LC3 (2220, Novus Biologicals, WB: 1:1,000), mouse anti‐p62 (610833, BD Biosciences, IF: 1:1,000), rabbit anti‐p62 (18420‐1‐AP, ProteinTech, IF: 1:200), rabbit anti‐FIP200 (SAB4200135, Sigma, WB: 1:500), rabbit anti‐ULK1 (#8054, Cell Signaling, WB: 1:1,000), rabbit anti‐phospho‐ULK S757 (#6888, Cell Signaling, WB: 1:1,000), mouse anti‐EGFP (JL8, Clontech, WB: 1:5,000), rabbit anti‐ATG13 (#6940, Cell Signaling, WB: 1:1,000), rabbit anti‐Rab1a (Ab97956 Abcam, WB: 1:1,000), and rabbit anti‐HA (Sigma, WB: 1:2,000, IF: 1:1,000). Secondary antibodies used for immunoblotting were horseradish peroxidase‐coupled goat anti‐rabbit, goat anti‐rat, and rabbit anti‐mouse IgG (Dako; 1:5,000). Secondary antibodies used for immunofluorescence were Alexa fluorophore (488, 568, or 633)‐coupled goat/donkey anti‐mouse IgG, Alexa fluorophore (488, 568, or 633)‐coupled goat/donkey anti‐rabbit IgG (Invitrogen; 1:500).
Webster C.P., Smith E.F., Bauer C.S., Moller A., Hautbergue G.M., Ferraiuolo L., Myszczynska M.A., Higginbottom A., Walsh M.J., Whitworth A.J., Kaspar B.K., Meyer K., Shaw P.J., Grierson A.J, & De Vos K.J. (2016). The C9orf72 protein interacts with Rab1a and the ULK1 complex to regulate initiation of autophagy. The EMBO Journal, 35(15), 1656-1676.
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