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Scd163

Manufactured by R&D Systems
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The SCD163 is a lab equipment product from R&D Systems. It is a specialized device designed for research applications. The core function of the SCD163 is to perform a specific task within a laboratory setting. No further details on the intended use or interpretation of the product's capabilities are provided.

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Lab products found in correlation

Scd14, by R&D Systems (11 mentions) Interleukin 6 (il 6), by R&D Systems (4 mentions) I fabp, by R&D Systems (3 mentions) Ip 10, by R&D Systems (2 mentions) Hscrp, by R&D Systems (2 mentions) C reactive protein (crp), by R&D Systems (2 mentions) Elx808 elisa reader, by Agilent Technologies (1 mentions) Spd 1, by R&D Systems (1 mentions) Scd27, by Thermo Fisher Scientific (1 mentions) Zonulin, by PromoCell (1 mentions) Stnfri, by R&D Systems (1 mentions) Endpoint limulus amebocyte lysate assay, by Associates of Cape Cod (1 mentions) Soluble tissue factor, by R&D Systems (1 mentions) C reactive protein (crp), by Thermo Fisher Scientific (1 mentions) I fabp, by Hycult Biotech (1 mentions) Spd l1, by R&D Systems (1 mentions) Sgp130, by R&D Systems (1 mentions) Sil 6r, by R&D Systems (1 mentions) Softmax pro version 5, by Molecular Devices (1 mentions) Furin, by Merck Group (1 mentions)

25 protocols using scd163

1

Plasma Biomarker Quantification Protocol

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Stored plasma samples from all 4 timepoints were thawed and analyzed in batch. Commercially available ELISA kits were used to determine the plasma concentrations of soluble CD14 (sCD14; R&D, Minneapolis, MN), soluble CD163 (sCD163; R&D), interleukin 6 (IL-6; R&D), interferon gamma-induced protein 10 (IP-10; R&D), and C-reactive protein (CRP; R&D) according to manufacturer’s instructions. Duplicates of 20% of the samples were included in each ELISA plate. Results were analyzed using an ELX808 ELISA reader (Biotek, Vinooski, VT) using Gen5 software v2.06.
Agudelo-Hernandez A., Chen Y., Bullotta A., Buchanan W.G., Klamar-Blain C.R., Borowski L., Riddler S.A., Rinaldo C.R, & Macatangay B.J. (2017). Subclinical Herpesvirus Shedding Among HIV-1-Infected Men on Antiretroviral Therapy. AIDS (London, England), 31(15), 2085-2094.
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2

Immune Biomarker Profiling by ELISA

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Plasma levels of sCD14 (Bio-Rad), sCD163 (R&D), sPD-1 (R&D) and Platelet derived growth factor (PDGF; Biorad) were measured by ELISA.
George P.J., Kumar N.P., Sridhar R., Hanna L.E., Nair D., Banurekha V.V., Nutman T.B, & Babu S. (2014). Coincident Helminth Infection Modulates Systemic Inflammation and Immune Activation in Active Pulmonary Tuberculosis. PLoS Neglected Tropical Diseases, 8(11), e3289.
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3

Quantification of Soluble Immune Markers

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Commercially available ELISA kits were used to detect sCD14 (R & D Systems, Minneapolis, MN), sCD27 (eBioscience, San Diego CA), and sCD163 (R & D Systems, Minneapolis, MN) within previously frozen plasma stored at −80°C. sCD14 was measured at a dilution factor 1:200 with a detection range of 250 – 8000 pg/ml; sCD27 at a dilution factor of 1:50, detection range 0.31 – 20 U/ml; and sCD163 at a dilution factor of 1:20, detection range of 1.6 – 100 ng/ml. The ELISA plates were read on Biotek® EL800 automated microplate reader (Winooski, VT) and results were analyzed using KCjunior™ microplate data analysis software, version 1.41.5 (Biotek®, Winooski, VT).
Rudy B.J., Kapogiannis B.G., Worrell C., Squires K., Bethel J., Li S., Wilson C.M., Agwu A., Emmanuel P., Price G., Hudey S., Goodenow M.M, & Sleasman J.W. (2015). Immune reconstitution but persistent activation after 48 weeks of antiretroviral therapy in youth with pre-therapy CD4 >350 in ATN 061. Journal of acquired immune deficiency syndromes (1999), 69(1), 52-60.
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4

Quantification of Serum sCD14 and sCD163

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Commercial antibody pairs were used to assess serum levels of soluble CD14 (sCD14) and soluble CD163 (sCD163) (R & D Systems, Minneapolis, MN, USA) in accordance with the manufacturer’s instructions. Briefly, half volume ninety-six well plates (Corning, Corning, NY, USA) were coated with the antibodies to the soluble marker of interest and incubated overnight at 4 °C. Plates were washed with 0.05% Tween/PBS and blocked with 1% BSA/PBS for 1 h to prevent non-specific binding. Standards and test sera were added to the plates and incubated for 2 h. Plates were washed and incubated with biotinylated antibody. After 2 h, plates were washed and incubated with Streptavidin-HRP for 20 min. Plates were washed again, TMB substrate was added, and the plates were left in the dark for colour development. The enzyme was inactivated via addition of H2SO4, and the absorbance was read on the spectrophotometer at 450 nm. The coefficients of variance for each assay were sCD14 (6.5%) and sCD163 (10.5%).
Ahmad A.F., Caparrós-Martín J.A., Lee S., O’Gara F., Yeap B.B., Green D.J., Ballal M., Ward N.C, & Dwivedi G. (2023). Gut Microbiome and Associated Metabolites Following Bariatric Surgery and Comparison to Healthy Controls. Microorganisms, 11(5), 1126.
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5

Biomarker Quantification in Gut Inflammation

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Zonulin (Promocell Germany), BDG (Mybiosource Inc. CA), I-FABP, sCD14, sCD163, sTNFRI, hsCRP, IL-6 (R &D Systems, Minneapolis, Minnesota, USA) and oxidized LDL (ALPCO, Salem, New Hampshire, USA and Mercodia, Uppsala, Sweden) were measured by ELISA . The intra-assay variability ranged between 4-8% and inter-assay variability was less than 10% for all markers. All assays were done at Dr Funderburg’s laboratory at Ohio State University, Columbus, OH (https://u.osu.edu/caffre/people/nicholas-t-funderburg-ph-d/). Laboratory personnel were blinded to group assignments.
TOE S., NAGY M., ALBAR Z., Yu J., SATTAR A., NAZZINDA R., MUSIIME V., ETAJAK S., WALYAWULA F., MCCOMSEY G.A., ATUYAMBE L.M, & DIRAJLAL-FARGO S. (2022). Ambient Air Pollution is Associated with Vascular Disease in Ugandan HIV positive Adolescents. AIDS (London, England), 36(6), 863-870.
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6

Quantifying Biomarkers in HIV Patients

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Plasma soluble CD163 (sCD163), monocyte chemoattractant protein-1 (MCP-1), interleukin (IL)-6, and sCD14 levels were quantified by enzyme-linked immunosorbent assay (ELISA) (Trillium Diagnostics [sCD163] and R&D Systems [high-sensitivity IL-6, MCP-1, and sCD14]). Endpoint Limulus amebocyte lysate assay (Associates of Cape Cod) was used to measure lipopolysaccharide levels. Total cholesterol, HDL, low-density lipoprotein, and triglycerides were determined using standard techniques. C-reactive protein was measured using ELISA. Human immunodeficiency virus RNA was measured in real time using clinically available reverse-transcription polymerase chain reaction assays. The main objective was to assess large reductions in extreme viremia for which clinically available assays were sufficient. Over the course of this clinical trial, methods to quantify viremia in the MGH Microbiology laboratory (Virology Quality Assurance certified) evolved as newer, more sensitive assays became available (see also Supplementary Methods). In an exploratory analysis, HIV Nef was quantified by ELISA (Immunodiagnostics). In the analysis of change in Nef, full data from baseline and 12 weeks were available from 6 subjects in the ART group and 6 subjects in the untreated group. Methods to assess effects of ART on efflux capacity and to assess endothelial function are included in Supplementary Methods.
Lo J., Rosenberg E.S., Fitzgerald M.L., Bazner S.B., Ihenachor E.J., Hawxhurst V., Borkowska A.H., Wei J., Zimmerman C.O., Burdo T.H., Williams K.C., Freeman M.W, & Grinspoon S.K. (2014). High-Density Lipoprotein-Mediated Cholesterol Efflux Capacity Is Improved by Treatment With Antiretroviral Therapy in Acute Human Immunodeficiency Virus Infection. Open Forum Infectious Diseases, 1(3), ofu108.
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7

Multiplex Biomarker Profiling in Plasma

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Concentrations of IL-1β, IL-1Ra, IL-6, IL-8, IL-10, IL-12p40, IL-12p70, IL-15, IL-17, IL-18, CCL2, CCL3, CCL4, CCL5, CCL11, CXCL10, IFN-γ, TNF-α, TGF-β, platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF) (Bio-Plex, Bio-Rad, Hercules, CA), C-reactive protein (CRP) (eBioscience, San Diego, CA), sCD163, soluble tissue factor (sTF) (R&D Systems, Minneapolis, MN) and intestinal fatty acid binding protein (I-FABP) (Hycult Biotech, The Netherlands) were assessed in cryopreserved plasma samples maintained at −80 °C.
Silveira-Mattos P.S., Narendran G., Akrami K., Fukutani K.F., Anbalagan S., Nayak K., Subramanyam S., Subramani R., Vinhaes C.L., Souza D.O., Antonelli L.R., Satagopan K., Porter B.O., Sher A., Swaminathan S., Sereti I, & Andrade B.B. (2019). Differential expression of CXCR3 and CCR6 on CD4+ T-lymphocytes with distinct memory phenotypes characterizes tuberculosis-associated immune reconstitution inflammatory syndrome. Scientific Reports, 9, 1502.
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8

Soluble Immune Marker Profiling

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Levels of sTARC, sCD163, sPD‐1, sPD‐L1, sPD‐L2, sVEGF (R&D systems) and sCD83 (Sino Biological; Beijing, China) were measured with a sandwich enzyme‐linked immunosorbent assay (ELISA), according to the manufacturer’s instructions.
Veldman J., Alsada Z.N., van den Berg A., Plattel W.J., Diepstra A, & Visser L. (2021). Soluble PD‐L1 is a promising disease biomarker but does not reflect tissue expression in classic Hodgkin lymphoma. British Journal of Haematology, 193(3), 506-514.
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9

Multiplex Cytokine and Chemokine Analysis

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The concentrations of IL-6, sIL-6R, sgp130, sCD163 (R&D Systems, Minneapolis, MN, USA), and soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK; eBioscience, Vienna, Austria) were determined using commercially available ELISA kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. The mean minimum detection limit was 0.039 pg/mL for IL-6 High Sensitivity, 6.5 pg/mL for sIL-6R, 0.08 ng/mL for sgp130, 9.7 pg/mL for sTWEAK, and 0.177 ng/mL for sCD163. ELISA assays recognized both free cytokines and their complexes.
The chemokines’ concentration analysis was conducted in patients treated with CRT with multiplex technique of the commercially available panel of 40 inflammatory chemokines (Human Chemokine Array Q1, RayBiotech Inc., Peachtree Corners, GA, USA), as previously described [17 (link)]. This method is based on the specific reaction of antibodies with the chemokine molecules. The visualization of the chemokine–antibody–biotin complex is performed by laser scanner (GenePix 4100A, Axon Instruments Inc., Foster City, CA, USA).
Ptaszyńska-Kopczyńska K., Eljaszewicz A., Marcinkiewicz-Siemion M., Sawicka-Śmiarowska E., Tarasiuk E., Lisowska A., Tynecka M., Grubczak K., Radzikowska U., Janucik A., Moniuszko M., Charkiewicz K., Laudański P., Sobkowicz B, & Kamiński K.A. (2021). Monocyte Subsets in Patients with Chronic Heart Failure Treated with Cardiac Resynchronization Therapy. Cells, 10(12), 3482.
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10

Quantifying Biomarkers in Periodontal Disease

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ELISA was used for the quantitative detection of B2M (Abcam, Cambridge, MA, USA), PGE2 (R&D Systems, Minneapolis, MN, USA), and CTXI (Cloud-Clone Corp, Houston, TX, USA) from GCF samples, and for sCD14 (R&D Systems, Minneapolis, MN, USA) and sCD163 (R&D Systems, Minneapolis, MN, USA) detection from serum samples. B2M was assayed at 1:40, PGE2 at 1:20, CTXI at 1:2 dilution, sCD163 as neat (undiluted) and sCD14 at 1:200, following manufacturer’s instructions, respectively. Data were analyzed using SoftMax Pro version 5 ELISA software (Molecular Devices, Sunnyvale, CA). The lowest value detected or extrapolated by the proprietary software was used as the minimum detectable value. Concentrations lower than extrapolated values calculated were considered out of range.
Moscicki A.B., Yao T.J., Russell J.S., Farhat S., Scott M., Magpantay L., Halec G., Shiboski C.H, & Ryder M.I. (2019). Biomarkers of oral inflammation in perinatally HIV-infected and perinatally HIV exposed, uninfected youth. Journal of clinical periodontology, 46(11), 1072-1082.
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