N hexane

In this study, the immobilized lipases used were Novozym 435 (Candida antarctica lipase B immobilized on an acrylic resin), Lipozyme RM IM (Rhizomucor miehei lipase immobilized on an anionic resin), and Lipozyme TL IM (Themomyces lanuginose lipase immobilized on silica gel). All enzymes were purchased from Novozymes (Bagsvaerd, Denmark). The solvent reactants used were acetonitrile (99.5%), toluene (99.5%), and n-hexane (96%) purchased from Junsei (Tokyo, Japan), as well as acetone (99.5%), tetrahydrofuran (THF), 1,2-dichloroethane (99%), cyclohexane (99.5%), n-heptane (98%), and iso-octane (98%) purchased from Dae-jung (Gyunggido, Korea).

Acetonitrile, 2-propanol, ampicillin, tributyrin, p-nitrophenyl (p-NP) acetate (C₂), p-NP butyrate (C₄), p-NP caprylate (C₈), lithium chloride, nickel chloride, sodium dodecyl sulfate, dithiothreitol, 2-mercaptoethanol, Triton X-100 and phenylmethylsulfonyl fluoride were purchased from Sigma Aldrich Co. (USA). Dimethyl sulfoxide, cobalt chloride hexahydrate, magnesium sulfate, potassium chloride, calcium chloride, sodium chloride, zinc chloride, chloroform, cyclohexane, n-hexane and acetone were purchased from Junsei Co. (Japan). p-NP caproate (C₆) was purchased from Tokyo Chemical Industry Co. (Japan). Methanol and ethanol were purchased from Merck Chemical Co. (Germany), and β-D-thiogalactopyranoside was purchased from Duchefa Biochemie B.V. Co. (The Netherlands). Copper sulfate was purchased from Kanto Chemical Co. (Japan). X-gal was purchased from Promega (USA). Ni-NTA agarose was purchased from Qiagen (Germany). We were provided with a lipolytic bacterial strain (No. 39-G1) isolated from the Arctic Beaufort Sea (70-56-1581 N, 136-25-1178 W) by Korea Polar Research Institute.