Photolysis
of PetACrONO was
carried out in isobutyronitrile solution. The NO release was determined
by entraining the photolysis solution contained in a “Y”
cell with medical-grade air, which then flowed to a Sievers Nitric
Oxide Analyzer (NOA, GE model
NOA-280i), where it was detected and
quantified via a calibrated chemiluminescence technique (SI
Figure S1). (In each case, the entraining gas
was purged through a solvent bubbler prior to entering the photolysis
cell to reduce evaporative losses.) The photoexcitation source was
either the 365 nm emission from a 200 W high-pressure mercury lamp
isolated with an interference filter or the 451 nm light from a Luxeon
blue light-emitting diode (LED) (SI
Figure S2). Excitation intensities at 365 nm were determined by ferrioxalate
actinometry,
47 (link) while those at 451 nm were
determined with a power meter (Newport model 8442 PE). Excitation
intervals were controlled using a Uniblitz shutter, and quantum yields
of NO release (Φ
NO) were determined by evaluating
the NO released and recorded using the NOA software (“Liquid”)
for varied excitation intervals. The slope of a plot of NO released
vs total light absorbed by the solution gives Φ
NO (SI
Figure S3).
Huang P.J., Garcia J.V., Fenwick A., Wu G, & Ford P.C. (2019). Nitric Oxide Uncaging from a Hydrophobic Chromium(III) PhotoNORM: Visible and Near-Infrared Photochemistry in Biocompatible Polymer Disks. ACS Omega, 4(5), 9181-9187.
