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3 protocols using gallocatechin

1

Antioxidant Compounds Characterization

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(+)-Sodium L-ascorbate (≥98%), α-ketoglutaric acid sodium salt (≥98%), iron(II) sulfate heptahydrate (≥99%), catalase from bovine liver (powder), (±)-naringenin (≥95%), (+)-taxifolin (2R,3R-dihydroquercetin or (+)-DHQ, ≥85%), kaempferol (≥95%), quercetin (≥95%), myricetin (≥96%), (−)-catechin (2S,3R-catechin, ≥98%), and methanol (HPLC grade) were all purchased from Sigma-Aldrich. 2S-Naringenin (≥98%), (+)-afzelechin (2R,3S-afzelechin, ≥98%) and (+)-gallocatechin (2R,3S-gallocatechin, ≥98%) were purchased from ChemFaces (Wuhan, China). (+)-Catechin (2R,3S-catechin, ≥99%), (−)-epicatechin (2R,3R-epicatechin, ≥99%), cyanidin chloride (≥96%) and delphinidin chloride (≥97%) were purchased from Extrasynthèse (Genay, France). (+)-Dihydrokaempferol (2R,3R-dihydrokaempferol or (+)-DHK, ≥95%) was purchased from ArboNova (Turku, Finland). (+)-Dihydromyricetin (2R,3R-dihydromyricetin or (+)-DHM) was purchased from Sequoia Research Products Ltd. (Pangbourne, Berkshire, UK).
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2

HPLC Analysis of Polyphenol Compounds

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Epicatechin (EC), gallocatechin (GC), catechin gallate (CG), gallocatechin gallate (GCG), epigallocatechin (EGC), Epicatechin gallate (ECG) and catechin (C) were purchased from Chemfaces with purity >98%. Epigallocatechin gallate (EGCG) with guaranteed grade was purchased from Nacalai Tesque. A high-performance liquid chromatography (HPLC) grade acetonitrile was obtained from JT Baker. HepG2 cells (ATCC® HB-8065™) were obtained from Institute of Marine Biotechnology, Universiti Malaysia Terengganu. Alpha-minimum essential media (α-MEM) with SP grade, Penicillin-Streptomycin solution with SP grade and MTT powder with guaranteed reagent (GR) grade were obtained from Nacalai Tesque. Fetal bovine serum (FBS) was obtained from Tico Europe. MEM non-essential amino acids solution, trypsin ethylenediaminetetraacetic acid (EDTA), trypan blue stain, phosphate buffered saline (PBS) tablet, dimethyl sulfoxide (DSMO) with AR grade and BODIPY FL LDL were obtained from Thermo Fisher Scientific. Lipid depleted FBS was obtained from Biowest. Cholesterol powder with purity ≥99%, 25-hydroxylcholesterol with purity ≥98% was purchased from Sigma-Aldrich, while berberine chloride (BC) was purchased from Merck.
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3

Synthesis and Characterization of AgNPs

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Streptozotocin and Silver sulfadiazine (AgS) were procured from Sigma Company St. Louis, MO, USA respectively. Gallocatechin was procured from Chem Faces, Wuhan, China. Silver nanoparticles (AgNPs; <150 nm; Purity: 99%,) purchased from Chem Cruz, Heidelberg, Germany.
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