Mouse anti bad

Manufactured by Santa Cruz Biotechnology

Mouse anti-BAD is a primary antibody that specifically recognizes the BAD (Bcl-2-associated death promoter) protein in mouse samples. BAD is a pro-apoptotic member of the Bcl-2 protein family, which plays a key role in regulating cell survival and apoptosis.

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2 protocols using mouse anti bad

Western Blot Analysis of Cell Signaling

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Immunoblot analysis was performed
as previously described^{58 (link)−60 (link)} using mouse anti-β-Actin,
mouse anti-p-ERK, rabbit anti-ERK, mouse anti-p-EGFR (Y1045), mouse
anti-p-EGFR (Y1148), mouse anti-p-EGFR (Y1173), mouse anti-CCND1,
mouse anti-CDK4, mouse anti-CCNB1, rabbit anti-c-MYC, mouse anti-BCL2,
mouse anti-BCL-XL, mouse anti-BAD, rabbit anti-CYCS, mouse anti-p-ERK
(44/42), and rabbit anti-ERK antibodies were procured from Santa Cruz
Biotechnology, CA. Mouse anti-CDH1, mouse anti-CDH2, rabbit anti-OCLN,
rabbit anti-pSTAT3, and mouse anti-STAT3 antibodies were obtained
from Abcam, Cambridge, MA. Rabbit anti-p-EGFR (Y992) and rabbit anti-p-CDK2
antibodies were obtained from Cell Signaling. Cell extracts were resolved
on SDS-PAGE and immunoblotted, with the appropriate and respective
antibodies. β-Actin was used as input control for cell lysate.
The sizes of the detected protein bands are shown in kilodaltons on
the left side.

Sebastian A., Pandey V., Mohan C.D., Chia Y.T., Rangappa S., Mathai J., Baburajeev C.P., Paricharak S., Mervin L.H., Bulusu K.C., Fuchs J.E., Bender A., Yamada S., B.a.s.a.p.p.a., Lobie P.E, & Rangappa K.S. (2016). Novel Adamantanyl-Based Thiadiazolyl Pyrazoles Targeting EGFR in Triple-Negative Breast Cancer. ACS Omega, 1(6), 1412-1424.

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Western Blot Analysis of Apoptosis Regulators

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Following SDS-PAGE, proteins were electroblotted onto Immobilon-P membranes. Following blocking in 5% dried skimmed milk (Marvel) dissolved in TBST (50 mM Tris pH 7.6, 150 mM NaCl, 0.1% Tween-20), membranes were incubated overnight at 4°C with the following primary antibodies diluted in TBST: 54H6 (Rabbit anti-Bcl-xL, 1 : 1000; Cell Signalling Technology), S-19 (Rabbit anti-Mcl-1, Santa Cruz Biotechnology), sheep anti-Tao1 (1 : 3000 [73 (link)]), rabbit anti-Bim (1 : 500; BD Biosciences), mouse anti-Bad (1 : 1000; Santa Cruz), rabbit anti-Bid (1 : 1000; Cell Signalling), mouse anti-Bax (1 : 1000; BD BioSciences), mouse anti-Bak (1 : 1000; Calbiochem), 4A6 (mouse anti-Myc tag; 1 : 1000; Millipore) and GFP (Rabbit anti-GFP; 1 : 1000; Cell Signalling). Membranes were then washed three times in TBST and incubated for at least 1 h with appropriate horseradish-peroxidase-conjugated secondary antibodies (Zymed). After washing in TBST, bound secondary antibodies were detected using either EZ-ECL Chemiluminescence Reagent (Biological Industries) or Luminata Forte Western HRP Substrate (Millipore) and a Biospectrum 500 imaging system (UVP).

Bennett A., Sloss O., Topham C., Nelson L., Tighe A, & Taylor S.S. (2016). Inhibition of Bcl-xL sensitizes cells to mitotic blockers, but not mitotic drivers. Open Biology, 6(8), 160134.

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