Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, albumin, creatinine, urea, and uric acids were purchased from Biodiagnostics Company (Dokki, Giza, Egypt). The RNA extraction Kit was purchased from Introbio Company, Cat.No. 17063. Reverse transcriptase Polymerase chain reaction (RT-PCR) sensiFAST™ cDNA synthesis kit (Bioline, United Kingdom). All used primers are commercially based. Aflatoxin from the Mycology and Mycotoxins Department, Animal Health Research Institute (ARC), Dokki, Egypt. Curcumin Nanoparticles (NT-Cur-NPs) were purchased from Nanotech Egypt for photo- electronics at the city of 6 October, Al Giza. Saccharomyces cerevisiae cell wall extract (100%, Beta-glucan: 20% minimum &Manan Oligosaccharides: 20% minimum.) was obtained from IBEX Group for animal nutrition and health products and produced by Shandong Bio Sunkeen Co., Ltd (a state-owned high-tech enterprise that integrates R&D, production and sales with rich experience of yeast and nature yeast products.
Urea
Manufactured by Biodiagnostic
Sourced in Egypt
Urea is a laboratory diagnostic product used to measure the concentration of urea in biological samples, such as blood or urine. Urea is a waste product formed in the liver as a result of the breakdown of proteins and is primarily excreted by the kidneys. The analysis of urea levels can provide valuable information about kidney function and overall metabolic processes.
Automatically generated - may contain errors
Lab products found in correlation
Creatinine, by Biodiagnostic (4 mentions)
Albumin, by Biodiagnostic (4 mentions)
Aspartate aminotransferase, by Biodiagnostic (2 mentions)
Malondialdehyde, by Biodiagnostic (2 mentions)
Catalase, by Biodiagnostic (2 mentions)
Alkaline phosphatase, by Biodiagnostic (1 mentions)
Total protein, by Biodiagnostic (1 mentions)
Sensifast cdna synthesis kit, by Meridian Bioscience (1 mentions)
Alanine aminotransferase, by Biodiagnostic (1 mentions)
Agarose, by Merck Group (1 mentions)
Acetaminophen, by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Qiazol for rna extraction, by Qiagen (1 mentions)
Rat abc staining system, by Santa Cruz Biotechnology (1 mentions)
Oligo dt primer, by Qiagen (1 mentions)
Aspartate transaminase, by Biodiagnostic (1 mentions)
Alanine transaminase, by Biodiagnostic (1 mentions)
Reduced glutathione, by Biodiagnostic (1 mentions)
Curcumin, by Merck Group (1 mentions)
Potassium dichromate k2cr2o7, by Merck Group (1 mentions)
7 protocols using urea
1
Hepatoprotective and Antioxidant Evaluation
2
Acetaminophen-Induced Liver Damage Evaluation
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Acetaminophen, ethidium bromide and agarose were purchased from Sigma-Aldrich (St. Louis, MO, USA). The Wistar albino rats were purchased from King Fahd center for Scientific Research, King Abdel-Aziz University, Jeddah, Saudi Arabia. Serologic kits for glutamate pyruvate transaminase (GPT), Glutamate oxalacetate transaminase (GOT), catalase, malondialdehyde (MDA), albumin and urea were purchased from Bio-diagnostic Co., Dokki, Giza, Egypt. The deoxyribonucleic acid (DNA) ladder was purchased from MBI, Fermentas, Thermo Fisher Scientific, USA. Qiazol for RNA extraction and oligo dT primer were purchased from QIAGEN (Valencia, CA, USA). Anti-MMP-8 primary antibody and rat ABC staining system were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
3
Biochemical Assays for Liver and Kidney Functions
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Kits for the enzymes aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), creatinine, and urea were purchased from Biodiagnostic Co., Egypt. The transaminase activities were determined calorimetrically as a change in absorbance/minute at the wavelength 505 nm, as described by Reitman and Frankel (1957 (link)). Serum ALP activity was detected colorimetrically at the wavelength 510 nm according to Belfield and Goldberg (1971 (link)). Creatinine was detected according to Bartels et al. (1972 ) and urea levels were measured using the method of Fawcett and Scott (1960 (link)). Serum total protein kits were obtained from Spectrum Diagnostics., Al-Obour city, Cairo, Egypt, and determined by the colorimetric method at 546 nm wavelength according to Sapan et al. (1999 (link)).
4
Curcumin and Renal Function Biomarkers
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Curcumin was purchased from Sigma-aldrich (St. Louis, MO, USA). Creatinine, urea, malondialdehyde (MDA), reduced glutathione (GSH) and phosphate buffer saline (PBS) were purchased from Bio-diagnostic Co (Dokki, Giza, Egypt). Thiopental sodium was supplied in the form of (Anapental 500 mg/vial), purchased from Sigma Tec Co., Egypt.
5
Antioxidant and Inflammation Evaluation
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Potassium dichromate (K2Cr2O7) [CAS# 7778-50-9] was purchased from Sigma-Aldrich Chemical Co. St. Louis, Missouri, (USA). Ethanol (96%; CAS #: 64-17-5) was purchased from Merck Millipore (MS, USA). Creatinine (cat# CR 12 51), Urea (cat# UR 21 10) and catalase (cat# CA 25 17) colorimetric kits were both purchased from Bio Diagnostic (Giza, Egypt), while the ELISA kits, including nuclear factor erythroid 2-related factor 2 (Nrf2, cat# SL0985Ra), prostaglandin E2 (PGE2, cat# SL0601Ra), cyclooxygenase 2 (COX-2, cat# SL0218Ra), UDP-glucuronosyltransferase (cat# SL1171Ra), mitogen-activated protein kinase (MAPK) (cat# SL1529Ra), and extracellular signal-regulated kinase (ERK) (cat# SL1390Ra), were all obtained from (SUNLONG BIOTECH CO., Ltd.), Hangzhou, China.
6
Ochratoxin-Induced Biochemical Alterations
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All chemicals used in this study were of analytical grade. Ochratoxin was purchased from Sigma Chemical Co. (St. Luis, MO, USA). Asp. Awamori powder was kindly supplied by Prof. K. Hayashi, Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan. The kits of aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein, albumin, urea, creatinine, malondialdehyde (MDA), catalase (CAT), and total antioxidant capacity (TAC) were obtained from Biodiagnostics Co. (Cairo, Egypt). While LDH kit was purchased from Randox Laboratories Ltd. (Crumlin, UK), and CK-MB was obtained from Stanbio™ (CK-NAC [UV-Rate] kit; Boerne, TX, USA).
7
Biochemical Markers Analysis in Research
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Kits for the determination of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma glut-aryl transaminase (GGT) were obtained from Biomed Diagnostics Co, Giza, Egypt. Pure acrylamide (99%) was purchased from Sigma Aldrich (St Louis, MO, USA). ELISA kits for analyzing cytokines were purchased from R&D (Mannheim, Germany). Kits for antioxidants (MDA, SOD, GSH, NO and catalase), urea, albumin, and total proteins were bought from Bio-diagnostics Co. (Cairo, Egypt). Reverse transcriptase enzymes for RNA and other related markers were bought from MBI (Fermentas, Thermo Fisher Scientific, USA). Oligo dT and Qiazol were purchased from QIAGEN Company (Valencia, CA, USA). Primers were designed and ordered from Macrogen CO. (Seoul, Korea).
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