μ slide 4 well glass bottom dish

For cell imaging experiments,
cells were planted on a μ-slide 4-well glass bottom dish (ibidi).
Cells were incubated with the DNA tetrahedron nanostructures after
being washed with phosphate-buffered saline. The three samples of
DNA tetrahedra (300 nM) were incubated with cells for 5 h and then
washed with DMEM/Hepes twice and replenished with the fresh medium
for the measurement. The fluorescence of tetrahedra in cells was monitored
with an Olympus FV3000 confocal laser scanning microscope, and all
images were analyzed with ImageJ.

Human
breast cancer cells (MCF-7) were
grown in 5% CO₂ RPMI-1640 medium supplemented with 10%
FCS, l-glutamine, and antibiotics (Biological Industries).
Human liver cancer cell line (HepG2) were grown in 5% CO₂ DMEM medium supplemented with 10% FCS, l-glutamine, and
antibiotics (Biological Industries). Normal breast cells (MCF-10A)
were maintained in complete growth medium consisting of 1:1 mixture
of Dulbecco’s modified Eagle’s medium and Ham’s
F12 medium supplemented with horse serum (5%), epidermal growth factor
(20 ng/mL), cholera toxin (CT, 0.1 μg/mg), insulin (10 μg/mL),
hydrocortisone (500 ng/mL), and penicillin/streptomycin (1 unit/mL).
Cells were plated 1 day prior to the experiment on a μ-slide
4-well glass bottom dish (ibidi) for confocal microscopy.