Goat anti mouse 568

Manufactured by Thermo Fisher Scientific

Sourced in United States

Goat anti-mouse 568 is a secondary antibody conjugated with the Alexa Fluor 568 fluorescent dye. It is designed to detect and visualize mouse primary antibodies in various immunodetection techniques.

Automatically generated - may contain errors

Lab products found in correlation

18 protocols using goat anti mouse 568

Immunofluorescence Microscopy of Mitotic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were used: rabbit anti-Aurora B (1:500; gift from Régis Giet), rabbit anti-Survivin (1:250; gift from Maria Grazia Giansanti), rat anti-αTubulin (1:500; Serotec). Secondary antibodies were from Molecular Probes and the Jackson Immuno Laboratory.
For three-dimensional structured illumination microscopy (3D-SIM), the following primary antibodies were used: mouse anti-αTubulin (1:2500; Sigma, DM1A) and rat anti-Miranda (1:1000; gift from Chris Doe). The following secondary antibodies were used: goat anti-mouse-568 (1:500; Molecular Probes) and donkey anti-rat Alexa Fluor 647 (1:300; Jackson Immuno).

Roth M., Roubinet C., Iffländer N., Ferrand A, & Cabernard C. (2015). Asymmetrically dividing Drosophila neuroblasts utilize two spatially and temporally independent cytokinesis pathways. Nature Communications, 6, 6551.

+ Open protocol

+ Expand

Quantifying Neurogenesis with BrdU and DCX

Check if the same lab product or an alternative is used in the 5 most similar protocols

BrdU labelling and BrdU/NeuN double-labelling: Brain slices were washed (PBS), then DNA was denatured with 2N HCl for 20 min at 37 °C, subsequent neutralisation with 0.1 M borax buffer (pH9) was 20 min at room temperature. Then, tissue was incubated with 10% fetal calf serum for 1 h RT. Primary antibodies (rat anti-BrdU, AbD Serotec, UK, 1:300; mouse anti-NeuN, Chemicon, Cork, Ireland, 1:500 or mouse anti-BrdU, BD Bioscience, UK, 1:100) were diluted in blocking solution. Primary antibodies were incubated overnight at 4 °C. The respective secondary antibodies (goat anti-rat 488, goat antimouse 568; goat anti-mouse 488, goat anti-rabbit 568, all Molecular Probes (Oregon, USA), 1:1,000 in blocking solution) were added for 2 h at room temperature following washing. Doublecortin labelling: Brain slices were permeabilised with 0.1% Triton X-100/PBS for 15 min on ice for DCX labelling procedures. Following PBS washes, slices were blocked in 5% horse serum and 0.3% Triton X-100/PBS for 30 min at room temperature. Slices were washed in PBS and goat anti-DCX antibody (Santz Cruz, 1:100, Santa Cruz Biotech, Germany) was applied in blocking solution overnight at 4 °C. After washing, the secondary antibody, donkey-anti-goat rhodamine conjugated (Jackson Immunoresearch, USA, 1:1,000) was applied for 2 h at room temperature followed by PBS washes.

Bunk E.C., König H.G., Prehn J.H.M, & Kirby B.P. (2020). p53 upregulated mediator of apoptosis (Puma) deficiency increases survival of adult neural stem cells generated physiologically in the hippocampus, but does not protect stem cells generated in surplus after an excitotoxic lesion. Journal of basic and clinical physiology and pharmacology, 32(2).

+ Open protocol

+ Expand

Double Labeling of BrdU and DCX in Brain Slices

Check if the same lab product or an alternative is used in the 5 most similar protocols

A combination of the protocols was used for the double-labelling of BrdU and doublecortin (DCX). Brain slices were stained for DCX using goat anti-DCX and donkey-anti-goat fluorescein conjugated antibodies. Following washing, slices were then prepared for BrdU labelling and mouse anti-BrdU (BD Bioscience, 1:100) was used as primary antibody and goat anti-mouse 568 (Molecular probes, 1:1,000) was used as secondary antibody. Slices were embedded in DAPI-containing mounting media or incubated with Hoechst 33342, and then washed and mounted in FluorSave Reagent (Calbiochem, Merck Bioscience, UK). Control experiments were performed by incubation with secondary antibodies only and no unspecific staining was observed.

+ Open protocol

+ Expand

Antibody Staining and Immunohistochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibody staining and immunohistochemistry were performed as previously described (Wang et al., 2004 (link)). The following primary antibodies were used: rabbit anti-GFP (Invitrogen, Carlsbad, CA, 1:1,000), mouse anti-GFP (Invitrogen, Carlsbad, CA, 1:1,000), mouse anti-nc82 (Hybridoma Bank, Iowa City, IA, 1:500), rabbit anti-RFP (Clontech, Mountain View, CA, 1:500); rabbit anti-GABA (Sigma-Aldrich, St. Louis, MO, 1:1,000); rabbit anti-FruM (1:100). For GRASP experiments, we used a mouse monoclonal antibody that specifically recognizes reconstituted GFP (Sigma-Aldrich, St. Louis, MO, 1:200). Secondary antibodies were Alexa Fluor goat anti-mouse 488, goat anti-rabbit 488, goat anti-mouse 568, goat anti-rabbit 568 (Life Technologies, Carlsbad, CA, 1:100).

Kallman B.R., Kim H, & Scott K. (2015). Excitation and inhibition onto central courtship neurons biases Drosophila mate choice. eLife, 4, e11188.

+ Open protocol

+ Expand

Pulse-Chase Antibody Labeling of Live Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Live cells were pulse-labeled with HA-antibody (1:500 dilution in staining media) as previously described. Following a 45-minute pulse on ice, cells were washed and then fixed or chased. A secondary Donkey-anti-Mouse-800 nm (LiCOR®, cat#926-32212, Lincoln, NE) or Goat-anti-Mouse-568 (LifeTechnologies, cat# A-11004, Carlsbad, CA) was used for IR scanning or confocal microscopy, respectively.

Snyder J.C., Pack T.F., Rochelle L.K., Chakraborty S.K., Zhang M., Eaton A.W., Bai Y., Ernst L.A., Barak L.S., Waggoner A.S, & Caron M.G. (2015). A rapid and affordable screening platform for membrane protein trafficking. BMC Biology, 13, 107.

+ Open protocol

+ Expand

Immunofluorescence Staining of Skin Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Slides were incubated at 60°C for 1 h and dewaxed by Bio‐Clear washing (Bio‐Optica) and sections rehydrated by 100, 90, 80, 70 and 50% ethanol incubations. Antigen retrieval was performed by boiling in 0.01 M Sodium Citrate Buffer pH 6.0. IF staining was as follows: permeabilization by Triton X‐100 0.2% PBS incubation; 1‐h blocking in 5% goat serum (Gibco) in PBS at RT; 3‐h primary antibody incubation at RT; and 1‐h secondary antibody and DAPI (4′,6‐diamidino‐2‐phenylindole) incubation at RT. IF images were acquired by Nikon A1 confocal laser microscope. The following antibodies were used: anti‐K14 (Abcam, ab7800); anti‐K14 (BioLegend, PRB‐155P); anti‐FLG (Santa Cruz, SC66192); anti‐LOR (BioLegend, PRB‐145P); anti‐Ki67 (Cell Signaling, D3B5, 9129S); anti‐p63 (Abcam, ab735); Alexa Fluor goat anti‐rabbit 568 (Life Technologies, A11011); goat anti‐rabbit 488 (Life Technologies, A11034); goat anti‐mouse 568 (Life Technologies, A11019); and goat anti‐mouse 488 (Life Technologies, A11017).

Panatta E., Lena A.M., Mancini M., Smirnov A., Marini A., Delli Ponti R., Botta‐Orfila T., Tartaglia G.G., Mauriello A., Zhang X., Calin G.A., Melino G, & Candi E. (2020). Long non‐coding RNA uc.291 controls epithelial differentiation by interfering with the ACTL6A/BAF complex. EMBO Reports, 21(3), e46734.

+ Open protocol

+ Expand

Immunofluorescent Staining of Mouse Brain Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were perfused with PBS followed by 4% paraformaldehyde 1 month after virus injection and brains were removed and dehydrated in 30% sucrose. 40 µm coronal brain sections were collected on a Leica cryostat. The immunofluorescence staining was performed as described before [40 (link)]. The primary anti-mouse MAP2 antibody (Sigma, 1:500) and the secondary antibody goat anti-mouse 568 (Invitrogen, 1:500) were applied sequentially. The slides were studied on a Zeiss microscope.

Lin L., Liu X., Cheng X., Li Y., Gearing M., Levey A., Huang X., Li Y., Jin P, & Li X. (2023). MicroRNA-650 Regulates the Pathogenesis of Alzheimer’s Disease Through Targeting Cyclin-Dependent Kinase 5. Molecular Neurobiology, 60(5), 2426-2441.

+ Open protocol

+ Expand

Retinal Whole Mount Immunohistochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Retinal whole mount immunohistochemistry was performed as previously described [23 ]. Briefly, the whole retina was immersed in 24-well plate (Sigma, HK) containing 200 μl 0.3% triton, 5% goat serum (sigma, HK)-PBS for 1 hour. Then the retina was incubated with rabbit-anti Iba1 (1:500, Wako, Japan) and mouse anti-beta-tubulin (1:200, Abcam, HK) for 24 hours at 4 degree. Then the retina was incubated with goat-anti rabbit 488 and goat-anti-mouse 568 (1:200, Invitrogen, HK) for 2 hours at room temperature for secondary antibody binding. Finally the retina was flat mounted on Dako (US) precoated slides for confocal imaging (Zeiss LSM 710, Germany).

Liang Y.X., Yang J., Yuan T.F, & So K.F. (2015). Uptake of Retrograde Tracers by Intact Optic Nerve Axons: A New Way to Label Retinal Ganglion Cells. PLoS ONE, 10(6), e0128718.

+ Open protocol

+ Expand

Multicolor Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sections were dewaxed 3 × 10 min in xylene and rehydrated in EtOH series (100%, 90%, 70%, 50%, and 30%) for a period of 2 min for each step. Antigen retrieval was performed in citric acid 0.01M at 90°C for 30 min. Blocking was achieved in PBS with 0.025% Tween20, 1% BSA, and 10% serum. After ON incubation at 4°C, the primary antibody was washed in PBT 0.025%, and a secondary antibody was incubated for 1 h at RT. After washing, the slides were mounted with fluoroshield. Primary antibodies were anti-acetylated tubulin 1/300 (Sigma-Aldrich, T7451), anti-Plunc1 1/200 (R&D systems, AF4274), and anti-K5 1/300 (Biolegend, PRB-160P), and secondaries were Goat anti-Mouse-568 1/500 (Invitrogen, A11004), Donkey anti-Sheep-488 1/500 (Invitrogen, A11015), and Donkey anti-Rabbit 647 1/500 (Invitrogen, A31573).
For the detection of PCNA 1/300 (Abcam, ab19166) and P63 1/400 (Abcam, ab124762), a secondary Goat anti-Rabbit biotin was used 1/800 (Dako, E0432) followed by Streptavidin-HRP (Abcam, ab64269). The color reaction was performed in TSA buffer (100 mM Borate buffer with 0.0003% hydrogen peroxidase) with Opal-570 1/300 (Akoya Bioscience, OP-001003) for 10min. DAPI was used to stain nuclei. For each genotype, N = 3.

Fons J.M., Milmoe N.J., Dack M.R., Joshi L., Thompson H, & Tucker A.S. (2022). The interconnected relationships between middle ear bulla size, cavitation defects, and chronic otitis media revealed in a syndromic mouse model. Frontiers in Genetics, 13, 933416.

+ Open protocol

+ Expand

Isolation and Culture of Neural Stem/Progenitor Cells

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

NSPCs were isolated from the pooled DG tissue dissected from two 6-month-old male mice using our published method [21 (link), 35 (link)]. NSPCs were cultured as described previously [35 (link)]. The proliferation and differentiation of NPCs were analyzed as described [20 (link)]. We used only early passage cells (between passages 4 and 10) and only the same passage numbers of wild-type and Fmr1 KO cells for data collection. For each experiment, triplicate wells of cells (experimental replicates) were analyzed, and the results were averaged as one data point (n = 1). At least three independent biological replicates (cells isolated from different animals) were used (n = 3) for statistical analyses.
The primary antibodies used were mouse anti-Tuj1 (1:1000, Covance, 435P) and rat anti-BrdU (1:3000, Abcam, ab6326).
Fluorescent secondary antibodies used were goat anti-mouse 568 (1:2000, Invitrogen, A11004) and goat anti-rat 568 (1:2000, Invitrogen, A11077).

Javadi S., Li Y., Sheng J., Zhao L., Fu Y., Wang D, & Zhao X. (2022). Sustained correction of hippocampal neurogenic and cognitive deficits after a brief treatment by Nutlin-3 in a mouse model of fragile X syndrome. BMC Medicine, 20, 163.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!