1440a digitizer

Manufactured by Molecular Devices

Sourced in United States

The 1440A digitizer is a high-performance data acquisition device designed for laboratory applications. It features a 16-bit analog-to-digital converter and can sample at rates up to 5 million samples per second. The 1440A provides multiple input channels and can capture and digitize analog signals with precision and accuracy.

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Lab products found in correlation

Pclamp 10, by Molecular Devices (3 mentions) Model 1700 differential ac amplifier, by A-M Systems (1 mentions) Mp 225, by Sutter Instruments (1 mentions) Mf 830, by Narishige (1 mentions) Axopatch 200b, by Molecular Devices (1 mentions) Pclamp software, by Molecular Devices (1 mentions) λ dna, by Takara Bio (1 mentions) Anotop filter, by Cytiva (1 mentions) Axopatch 700b, by Molecular Devices (1 mentions) Bx51wi, by Olympus (1 mentions) Multiclamp 700b amplifier, by Molecular Devices (1 mentions) Model 1700, by A-M Systems (1 mentions)

7 protocols using 1440a digitizer

Whole-cell Patch-clamp Recording Protocol

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The external solution is oxygenated Ames medium as used in two-photon imaging. Whole-cell recordings were made with patch pipettes (tip resistance 5–7 MΩ). Membrane current or potential was amplified, digitized at 10–20 kHz (Axopatch 700B amplifier; Digidata 1440 A Digitizer), stored, and analyzed by using pClamp 10.0 (Molecular Devices). Pipettes were filled with an intracellular solution composed of (in mM): 125 K-gluconate, 10 NaCl, 1 MgCl₂_, 10 EGTA, 5 HEPES, 5-ATP-Na, 0.1 GTP-Na (280 mOsm; pH adjusted to 7.4 with KOH). For gallein, BaCl₂, and tertiapin-Q experiments, 10 µM gallein, 10 µM BaCl_2, or 100 nM tertiapin-Q was added to the pipette solution. For the neurobiotin filling, 30 mM Neurobiotin was added to the pipette solution. Absolute voltage values were corrected for a liquid junction potential of −12.8 mV.

Jo A., Deniz S., Xu J., Duvoisin R.M., DeVries S.H, & Zhu Y. (2023). A sign-inverted receptive field of inhibitory interneurons provides a pathway for ON-OFF interactions in the retina. Nature Communications, 14, 5937.

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Diaphragm Electromyography in Anesthetized Mice

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Mice were anesthetized with 1.5% isofluorane gas in oxygen and head-fixed on a stereotaxic frame. A 26 gauge EMG needle (AMBU, Malaysia) was inserted through the skin, underneath the last rib into the thoracic diaphragm. Bioelectrical signals were amplified at a gain of 100, filtered between 10 Hz and 5 kHz (Model 1700 Differential AC Amplifier, A-M Systems) and digitized at 10 kHz (1440A digitizer and pClamp 10, Molecular Devices). With the help of an audio monitor (Model 3300, A-M Systems), recordings were initiated when the point of maximal MUAP was identified and each recording period lasted at least 60 s. The maximal amplitudes of MUAP bursts and the burst frequencies were quantified from each recording session and then averaged to obtain the single values of the MUAP amplitude and frequency for each animal, respectively.

Orengo J.P., van der Heijden M.E., Hao S., Tang J., Orr H.T, & Zoghbi H.Y. (2018). Motor neuron degeneration correlates with respiratory dysfunction in SCA1. Disease Models & Mechanisms, 11(2), dmm032623.

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Patch-Clamp Recordings of K+ and NSCC Currents in PASMCs

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K⁺ and NSCC current recordings were performed in freshly dispersed PASMCs by using conventional whole-cell patch-clamp configuration in voltage-clamp mode with the following biophysical criteria: seal resistance > 1 GΩ and series resistance < 25 MΩ. These criteria were confirmed after membrane rupture and monitored through the course of the experiment. Freshly isolated PASMCs were superfused in extracellular solution under constant flow (2 ml/min) at room temperature (~23°C). Whole-cell current data were generated with an Axopatch 200B amplifier (Axon Instruments) using 4- to 6-MΩ patch electrodes controlled by a motorized micromanipulator (MP-225, Sutter Instruments). Electrodes were pulled from 1.5-mm borosilicate filamented-glass (Sutter Instruments) with a micropipette puller (P-87, Sutter Instruments) and fire-polished with a microforge (MF-830, Narishige). Currents were filtered with a lowpass Bessel filter at 1 kHz and digitized (1440A Digitizer, Molecular Devices) and recorded using pClamp software (Molecular Devices). Currents were normalized to cell capacitance to obtain current density (pA/pF), and current-voltage (I–V) relationships were generated from the last 50 ms of each stimulus.

Jernigan N.L., Naik J.S, & Resta T.C. (2021). Acid-sensing ion channel 1 contributes to pulmonary arterial smooth muscle cell depolarization following hypoxic pulmonary hypertension. The Journal of physiology, 599(21), 4749-4762.

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Oscilloscope and Digitizer Setup for Electrophysiology

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Recording signals comprising potential and current traces were monitored on an HM-507 oscilloscope (Hameg, East Meadow, NY, USA) and digitally stored online at 10 kHz in a Sony VAIO CS series laptop computer (VGN-CS110E; Kaohsiung, Taiwan), equipped with 1440A digitizer (Molecular Devices). During the recordings with analog-to-digital and digital-to-analog conversion, the latter device was actually controlled by pCLAMP 10.7 software (Molecular Devices) run on Microsoft Windows 10 (Redmond, WA, USA). The laptop computer used was put on the top of an adjustable Cookskin stand (Ningbo, Zhejiang, China) for efficient manipulation during the experiments. To ensure digitalization, some of recordings were digitally collected by PowerLab 2/26 acquisition system (AD Instruments; Gerin Technology Co., Tainan, Taiwan). During measurements, we carefully exchanged the solutions between different sets of recordings through a home-made gravity-driven type of bath perfusion.

Chang W.T., Liu P.Y, & Wu S.N. (2020). High Capability of Pentagalloylglucose (PGG) in Inhibiting Multiple Types of Membrane Ionic Currents. International Journal of Molecular Sciences, 21(24), 9369.

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Nanopore Detection of λ-DNA

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λ-DNA (TaKaRa, Co., Ltd. Dalian, China) was diluted in 1 M KCl at a pH of 8.0. All other chemical reagents used in the nanopore experiment were of analytical grade and use without further purification. The samples were prepared with Milli-Q super purified water with a resistance of >18 MΩ/cm. All solutions were filtered with a 0.02 μm Anotop filter (Whatman, Co. Maidstone, Kent, UK) before using.
The DNA was detected by nanopore sensors. A patch clamp amplifier (Axon Instruments, Axopatch 700B) was used to measure the corresponding ionic current flowing through the nanopore as a function of the biased voltages. The sampling frequency was above 100 kHz with a low pass filter of 10 kHz cutoff frequency. The current signals were recorded by the 1440A digitizer (Molecular Devices, Inc. CA, USA). Data were collected over multiple experiments with the same nanopore. The whole nanopore device was set in a Faraday cage for shielding electromagnetic noise.

Wang Y., Yuan J., Deng H., Zhang Z., Ma Q.D., Wu L, & Weng L. (2022). Procedural Data Processing for Single-Molecule Identification by Nanopore Sensors. Biosensors, 12(12), 1152.

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Patch-Clamp Recording of Hippocampal Neurons

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The slices were placed in a recording chamber perfused with ACSF (2 ml/min) at 32-34 °C. Whole-cell patchclamp recordings of hippocampal neurons were obtained under 40X water-immersion lens (BX51WI, Olympus). Pipettes were pulled using a micropipette puller (P-97, Sutter instrument) with a resistance of 3-6 MΩ. Recordings were obtained using a MultiClamp 700 B amplifier and 1440 A digitizer (Molecular Device). For sEPSC and mEPSC recordings, glass pipettes were filled with a solution containing 125 mM Csmethanesulfonate, 5 mM CsCl, 10 mM Hepes, 0.2 mM EGTA, 1 mM MgCl2, 4 mM Mg-ATP, 0.3 mM Na-GTP, 10 mM phosphocreatine and 5 mM QX314 (pH 7.40, 285 mOsm). For sIPSC and mIPSC recordings, the holding potentials were 0 mV, glass pipettes were filled with a solution containing 110 mM Cs 2 SO 4 , 0.5 mM CaCl 2 , 2 mM MgCl 2 , 5 mM EGTA, 5 mM HEPES, 5 mM TEA, 5 mM ATP-Mg, pH 7.35, 285 mOsm.

Li X., Du Z.J., Xu J.N., Liang Z.M., Lin S., Chen H., Li S.J., Li X.W., Yang J.M, & Gao T.M. (2023). mGluR5 in hippocampal CA1 pyramidal neurons mediates stress-induced anxiety-like behavior. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 48(8).

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Electrophysiological Recordings from Diverse Preparations

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Signals from extracellular electrodes (EMG wires or glass suction electrodes) were amplified using an AM Systems model 1700 differential AC amplifier with frequency cut-offs at 1 Hz (low) and 10 kHz (high). Signals from ion-sensitive electrodes were amplified with a Duo773 amplifier (WPI Inc., Sarasota) using a high resistance headstage for the ion-specific electrode. Intracellular recordings were made with glass microelectrodes (20-50 MΩ, back-filled with 500 mM KCl and with 3 M KCl in the electrode holder) and amplified using a model 1600 Neuroprobe amplifier (A-M Systems). All electrophysiological signals were digitized (1440A digitizer; Molecular Devices) with a sampling rate of 100 kHz and recorded using Axoscope 10.7 for later analysis using Clampfit 10.7.

Robertson R.M., & Dusen R.A.V. (2021). Motor patterning, ion regulation and Spreading Depolarization during CNS shutdown induced by experimental anoxia in Locusta migratoria.

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