Nanostars were characterized using a Shimadzu GPC system comprising three KF-805L columns (300 × 8 mm, bead size: 10 mm, pore size maximum: 5000 Å) and SPD-20A UV/Vis and RID-10A differential refractive-index detectors. The temperature of columns was maintained at 40 °C. The eluent was N,N-dimethylacetamide (CHROMASOLV Plus for HPLC) with 0.03% w/v LiBr at a flow rate of 1.0 mL/min. A molecular weight calibration curve was produced using polystyrene standards (500–2 × 106 g mol−1). Polymer solutions (~2 mg/mL) were prepared and filtered through 0.45 μm PTFE filters before injection.
Kf 805l columns
Manufactured by Shimadzu
The KF-805L columns are a type of laboratory equipment used for chromatography applications. They are designed to facilitate the separation and purification of chemical substances. The core function of these columns is to provide a stationary phase for the separation process, allowing the components of a mixture to be separated based on their interactions with the stationary phase. The detailed specifications and intended applications of the KF-805L columns are not provided in this response.
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Lab products found in correlation
3 protocols using kf 805l columns
1
Gel Permeation Chromatography of Nanostars
2
Gel Permeation Chromatography Analysis
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GPC was performed
using a Shimadzu modular system comprised of a SIL-20AD automatic
injector, a RID-10A differential refractive-index detector, and a
50 × 7.8 mm guard column followed by three KF-805L columns (300
× 8 mm, bead size: 10 μm, pore size maximum: 5000 Å). N,N′ -Dimethylacetamide (DMAc, HPLC grade, 0.03%
w/v LiBr) at 50 °C was used for the analysis with a flow rate
of 1 mL min–1. Samples were filtered through 0.45
μm PTFE filters before injection. The GPC calibration was performed
with narrow-polydispersity polystyrene standards ranging from 500
to 2 × 106 g mol–1.
using a Shimadzu modular system comprised of a SIL-20AD automatic
injector, a RID-10A differential refractive-index detector, and a
50 × 7.8 mm guard column followed by three KF-805L columns (300
× 8 mm, bead size: 10 μm, pore size maximum: 5000 Å). N,N′ -Dimethylacetamide (DMAc, HPLC grade, 0.03%
w/v LiBr) at 50 °C was used for the analysis with a flow rate
of 1 mL min–1. Samples were filtered through 0.45
μm PTFE filters before injection. The GPC calibration was performed
with narrow-polydispersity polystyrene standards ranging from 500
to 2 × 106 g mol–1.
3
Polymer Characterization by NMR and SEC
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1 H Nuclear Magnetic Resonance ( 1 H NMR) spectroscopy of all samples was carried out using a Bruker AVANCE III HD 400 MHz spectrometer using deuterated solvents obtained from Sigma-Aldrich. Analyses of polymer solutions were performed using a Shimadzu modular system comprising a DGU-12A degasser, an SIL-20AD automatic injector, a 5.0 μm bead-size guard column (50 x 7.8 mm) followed by three KF-805L columns (300 x 8 mm, bead size: 10 μm, pore size maximum: 5000 Å), a SPD-20A ultraviolet detector, and an RID-10A differential refractive index detector. A CTO-20A oven was used to maintain the columns at 40 °C. N, Ndimethylacetamide (DMAc) with 0.03% w/v LiBr was used as the eluent where samples were run isocratically at 1 mL min -1 . Polystyrene standards (0.5 to 2000 kg mol -1 ) were used for calibration. Analyte samples were filtered through 0.45 μm PTFE filters before injection. Molar mass (Mn, SEC) and dispersity (Đ) values of samples were determined on Shimadzu LabSolutions software. Dynamic light scattering (DLS) analysis was carried out using a Malvern Zetasizer Nano ZS. All measurements were carried out at 25 °C using filtered polymer solutions of 2 mg mL -1 in PBS.
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