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2 protocols using allyl methacrylate

1

Synthesis of Methacrylate-Based Biomaterials

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2-Hydroxyethyl methacrylate (HEMA) was purchased from TCI Chemicals (Montgomeryville, PA). Ethylene glycol dimethacrylate (EGDMA), vinyl methacrylate (VM), 3-(Acryloyloxy)-2-hydroxypropyl methacrylate (AHPM), allyl methacrylate (AM), and 2,20-Azobis (2-methylpropionitrile) (AIBN) were purchased from Sigma Aldrich (St. Louis, MO). Materials were used as received. Human neonatal dermal fibroblasts were purchased from Cascade Biologics (Portland, OR).
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2

Synthesis of Functionalized Polymers

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Chemicals [2-(methacryloyloxy)ethyl]trimethylammonium chloride (METAC, 75 wt.% in H2O), 3sulfopropyl methacrylate potassium (SPMAK, 98%), 2-(dimethylamino)ethyl methacrylate (98%), 1,4-butane sultone (BS, ≥99%), 4-cyano-4-[(dodecylsulfanylthiocarbonyl)sulfanyl] pentanoic acid (CDPA, 97%), allyl methacrylate (AMA, 98%), n-butyl methacrylate (n-BMA, 99%), 3-mercaptopropyl trimethoxysilane (MPTMS, 95%), 2,2,2-trifluoroethanol (TFE, ≥99%), pentaerythritol tetrakis(3-mercaptopropionate) (PETMP, ≥95%), 4,4′-azobis(4-cyanovaleric acid) (ACVA, ≥98%), potassium chloride (KCl, 99.5%), potassium thiocyanate (KSCN, 99%), potassium bromide (KBr, 99%), lithium chloride (LiCl, 99%), and cesium chloride (CsCl, 98%) were purchased from Sigma-Aldrich Denmark. N,N-Dimethylformamide (≥99.9%), diethyl ether (≥99.9), toluene (≥99.8), and acetonitrile (≥99.9) were received from VWR Chemicals BDH and passed through activated basic aluminum oxide (Brockman I, Sigma-Aldrich) before use to remove any inhibitors. Zwitterionic monomer N-(2-methacryloylxyethyl)-N,N-diemthylammonium butanesulfonate (MABS) was prepared beforehand using the procedure reported in a previous study. 32 (link) Ultrapure water (Sartorius Arium Pro; 18.2 MΩ⋅cm) was used for preparing aqueous salt solutions.
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